EVALUATION OF CURRENT TECHNIQUES IN DIAGNOSES OF HUMAN IMMUNO DEFICIENCY VIRUS (HIV)

 

Table Of Contents


  • Title pageCertificationAcknowledgmentDedicationAbstractList of tablesCHAPTER ONE1.0 Introduction1.1 Hypothesis1.2 Aims and Objectives1.3 Statement of problem1.4 Limitation of studyCHAPTER TWO2.0 Literature review2.1 Pathogenesis of HIV infection2.2 Epidemiology of HIV2.3 Transmission of HIV infection2.4 Viral replication of HIV2.5 Symptoms of HIV infection2.6 Life cycle of HIV2.7 Facts about HIV/AIDSCHAPTER THREE3.0 Materials and Method3.1 Current diagnosis of HIV infection3.2 Laboratory diagnosis3.3 Radiomminoassay3.4 Enzymes Linked immunosorbentassay Elisa3.5 Western blotCHAPTER FOUR4.0 Results5.0 Discussion and conclusion References Appendices

Project Abstract

A study was carried to ascertain the potency of some techniques used in diagnosis of Human Immunodeficiency virus (HIV). Two hundred and five samples (203) were collected from the patients from the university of Nigerian teaching hospital and was analysed using Elisa kit, which has the ability to detect antibodies and antigens in the patients serum, the Western blots were used as the confirmatory test, to the Elisa test, In case where there is false positive of Elisa test, it is specific and sensitive, Radioimmunoasay looks for the antigens which are radioactively label. The patients result shows that 154 patients out of the 205 diagnosed were negative and only 51 patients were positive. The Elisa kit were best used because of its sensitivity and specificity. It is very cheap and can be easily seen in the market, unlike the Radiommunoassay which are rare and very expensive to be used.

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