Assessment of genetic (via protein electrophoresis) and nutritional variabilities among ten accessions of sphenostylis stenocarpa (hocht. ex. a. rich) harms

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of study
  • 1.3Problem Statement
  • 1.4Objective of study
  • 1.5Limitation of study
  • 1.6Scope of study
  • 1.7Significance of study
  • 1.8Structure of the research
  • 1.9Definition of terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Genetic Variability
  • 2.2Protein Electrophoresis as a Tool
  • 2.3Nutritional Variabilities in Plants
  • 2.4Accessions of Sphenostylis Stenocarpa
  • 2.5Previous Studies on Genetic and Nutritional Variabilities
  • 2.6Factors Influencing Genetic and Nutritional Variabilities
  • 2.7Relationship Between Genetic and Nutritional Variabilities
  • 2.8Importance of Studying Genetic and Nutritional Variabilities
  • 2.9Methods for Assessing Genetic and Nutritional Variabilities
  • 2.10Future Directions in Research

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Design and Justification
  • 3.2Selection of Study Population
  • 3.3Data Collection Methods
  • 3.4Sampling Techniques
  • 3.5Experimental Procedures
  • 3.6Data Analysis Techniques
  • 3.7Ethical Considerations in Research
  • 3.8Validation of Research Methods

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Overview of Data Analysis
  • 4.2Genetic Variabilities Among Accessions
  • 4.3Nutritional Variabilities in Sphenostylis Stenocarpa
  • 4.4Correlation Analysis Between Genetic and Nutritional Variabilities
  • 4.5Comparison with Previous Studies
  • 4.6Discussion on Factors Influencing Variabilities
  • 4.7Implications of Findings
  • 4.8Recommendations for Future Research

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of Findings
  • 5.2Conclusion and Interpretation
  • 5.3Contributions to the Field
  • 5.4Implications for Agriculture
  • 5.5Recommendations for Further Studies

Project Abstract

<p> The genetic variability among ten (10) accessions of Sphenostylis stenocarpa from three states and National Centre for Genetic Resources and Biotechnology Moor Plantation, Ibadan, Nigeria were assessed using sodium dodecyl polyacrylamide gel electrophoresis (SDS – PAGE). There were variations in the banding patterns of the accessions and polymorphism was also observed. Twelve polypeptide bands were obtained ranging from 9KDA to 219KDa. The Unweighted Pair Group Method with Mean Algorithm (UPGMA) dendrogram grouped the ten accessions into two clusters and six groups. Some accessions appeared in the same group, while others were observed to be in different groups. The proximate and nutritional analysis also detected variations. A strong and positive correlation was observed among some of the attributes studied. The principal component analysis also showed some variation among the accessions. The highest variability was observed in the ash content which shows that ash content could be a major indicator for nutritional variability in these 10 accessions followed by the Vitamin B2 in the second component. <br></p>

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