EFFECTS OF FERTILIZERS ON SOIL MICROBIAL DIVERSITY, GROWTH AND YIELD OF TWO FORAGE GRASSES ( Panicum maximum and Sorghum almum)
Table Of Contents
- Cover page Title page Certification Dedication Acknowledgement Abstract Organization of the work Table of Contents CHAPTER ONEINTRODUCTION-
- 11.1Justification for the Study- 2CHAPTER TWOLiterature Review-42.1Soil Microbial Diversity.-42.2Effects of Anthropogenic liquid waste on Soil microbes-52.3Effects of Chemical Fertilizers on Soil Microbes-52.4Effects of Cattle Dung on Soil Microbes-62.5Human Urine as a Fertilizer-6Response of Panicum maximum to Fertilization-72.7Response of Sorghum almum to Fertilization-
- 72.8Description of Test Crops-82.
- 8.1Panicum maximum -82.
- 8.2Sorghum almum -9CHAPTER THREE3.0Materials and Methods-103.1Experimental Site-103.2Collection of Soil Sample, Seed and Fertilizer-103.3Experimental Layout-
- 133.4Fertilizer Application-
- 133.5Data Collection-143.
- 5.1Sward height (cm)-143.
- 5.2Sward re-growth (cm) -143.
- 5.2Fresh herbage yield (g) -143.
- 5.3Dry herbage yield (g)-
- 143.6Statistical Analysis-
- 143.7Soil Sample for Microbial Analysis-153.
- 7.1Enumeration of micro-organism in soil samples from different treatments using the serial dilution method.-153.
- 7.2Equipment used:-153.
- 7.3Materials: -153.
- 7.4Medium used: -153.
- 7.5Media preparation-153.
- 7.6Preparation of sample: -163.
- 7.7Inoculation: -163.
- 7.8Incubation: -16CHAPTER FOURResults -18CHAPTER FIVEDiscussion-27CHAPTER SIXConclusion -27Reference-29LIST OF TABLESTable 1: Physical and chemical properties of the experimental soil-12Table 2: Effect of fertilizer and grass species on Sward Height at 2,4 and 6 weeks after sowing.....20Table 3: Effect of Fertilizer and grass species on Sward Regrowth at 2, 4 and 6 weeks after sowing -22Table 4:Effect of fertilizer and grass specie on herbage yield -24Table 5: Microbial population in the various treatments.-26 m
Project Abstract
A 6 month study was conducted in the screen house and laboratory of the Department of Crop Science, Faculty of Agriculture, University of Benin, Benin City. The objective was to ascertain the effects of fertilizers on soil microbial diversity growth and yield of two forage grasses. The experiment was set up in a Completely Randomized Design (CRD) arrangement. There were 10 factorial treatments, made up from five fertilizers (Control, Anthropogenic liquid waste (13 and 16 dilutions), NPK(151515) and cattle dung) and two forage grasses (Sorghum almum and Panicum maximum) replicated three times. The parameters measured were sward height, sward regrowth, fresh and dry herbage yields. Serial dilution of the fertilized soils was carried out to enumerate the microbial populations. Generally, anthropogenic liquid waste (13) and NPK(151515) were equal in performance and better than the other fertilizers in terms of herbage growth (sward height and sward regrowth). Anthropogenic liquid waste (13) produced the significantly highest fresh and dry herbage yields among the fertilizers. Furthermore anthropogenic liquid waste (13) favoured the highest proliferation of microbes. The favourable response of the grasses and the higher microbial population in the soils treated with Anthropogenic liquid waste implies that this fertilizer contains high quantities of readily available plant nutrients
Project Overview
INTRODUCTION
Soil is the habitat of an adverse array of organisms
that include both micro flora and micro fauna. Soil microorganisms play
an important role in soil fertility. They carry out biochemical
transformations and are also important sources and sinks of mineral
nutrients (Jenkison and Gadd, 1981). An understanding of microbial
processes is important for the management of farming systems that rely
on organic matter as source of nutrients (Smith and Paul, 1990). The
soil microbial community is involved in nutrient cycling, organic matter
decomposition and plays a crucial role in terrestrial carbon cycling
(Schimel, 1995).The microbial community is a very reactive ecosystem
with rapid growth and turnover (Panikov, 1999).
Fertilizer may
directly stimulate an increase in microbial population through nutrient
supply or may affect the composition of individual microbial communities
in the soil (Khonje et al.,1989). The application of chemical
fertilizer improves crop production while enhancing the activities of
soil microbes. However, there are concerns about the severe
environmental problems posed by fertilization and also about the long
term sustainability of inorganic fertilization (Mueder et al., 2002),
The
use of organic materials (cattle dung, poultry dung, crop residues etc)
as alternative sources of fertilizer holds promise. Cattle dung has the
potential benefit of improving soil structure (Reganoid et al., 1987;
Pullenman et al., 2003) and enhancing soil microbial activities (Doles
et al., 2001). However with the limited number of intensive livestock
farms and increase in commercial cropping systems, the use of cattle
dung as fertilizer has become inadequate. Furthermore, the prolonged
period of curing, ability to spread weeds and high labour required for
the application makes the use of cattle dung inadequate. There are other
organic materials that may be used for fertilization with less logistic
difficulties as those posed by cattle dung usage. Anthropogenic liquid
waste or human urine contains appreciable quantities of plant nutrients
especially nitrogen and other macro-nutrients in readily available
forms. Although there are social hesitations among Nigerians regarding
the use of human urine in agriculture, anthropogenic liquid waste has
been successfully used in agriculture in Asia (Wolgast,1993)
.
1.1 Justification for the Study
Due
to shortage in both inorganic and organic fertilizers, coupled with
indiscriminate disposal of human wastes in the environment, the
recycling of anthropogenic wastes for agriculture offers a promising
alternative source of fertilizer for crops. However, very little
research has been conducted with anthropogenic liquid wastes as
fertilizer for crops.
Tropical grasses like Panicum maximum and
Sorghum almum have the ability to produce high yield and high nutrient
quality when treated with fertilizer. Fertilization is one of the common
practices that can increase both forage production and nutritive value
(Harding, 2005). Anthropogenic liquid waste could be used to grow high
quality pasture grasses to complement current emphasis on increased
livestock and dairy cattle production in Nigeria. This study was
conducted to evaluate the effect fertilizers (cattle dung, NPK
fertilizer and anthropogenic liquid waste) on soil microbial diversity,
growth and yield of Panicum maximum and Sorghum almum .
OBJECTIVES OF THE STUDY
Effect of fertilizers on soil microbial diversity.
Effect of fertilizers on the growth and yield of Panicum maximum and Sorghum almum