MICROBIAL ANALYSIS OF EXPIRED CANNED FOOD PRODUCT (LIQUID CANNED MILK)

 

Table Of Contents


  • Title PageCertificationDedicationAcknowledgementAbstractCHAPTER ONE1.0 IntroductionAIMCHAPTER TWO2.0 Literature review
  • 2.1Types of dairy foods2.3 Types of spoilage micro organisms2.4 Soures of spolage microorganisms2.5 Contamination of dairy products2.6 Factors affecting spoilage2.7 Spoilage of cheeses2.9 Prevention and control measuresPrevention of spoilage in milk 2.
  • 9.0Prevention of spoilage in other dairy productsCHAPTER THREE3.0 Materials and methods3.1 Collection of sample3.2 Media used3.3 Sterilization of material3.4 Analysis of sample3.5 Identification of isolate3.6 Grams staining3.7 Catalase test3.8 Coagulase test CHAPTER FOURAnalysis and result
  • 4.0Analysis Result4.2 Table 2 colony morphology of isolates on Macconkey agar4.3 Tables 3 morphology of sub-culture from E.M.B culture E.M.B confirmatory plate4.4 Table 4: biochemical identification of bacteria isolates4.5 Changes in physical appearance of the expired canned milk samplesCHAPTER FIVE5.0 Discussion and conclusion5.1 Conclusion5.2 RecommendationReferences

Project Abstract

This study on the microbial assessment of expired canned milk products was conducted in Imo State between the months of June to October, 2012. a total of five expired canned milks were used in the study. The samples include expired Peak, Lunea, Nunu, Royal and Three Crown were analyzed using standard microbiological techniques ie pour plate and streak methods. The results of the study revealed the presence of staphylococcus aureus, Escheriachia coli and streptococci Spp. From the findings of the study, it is very clear that the consumption of expired milk products could serve as a potential sources of pathogenic micro-organism that could be lethal to life.

Project Overview

1.0 INTRODUCTION

MICROBIAL ANALYSIS OF EXPIRED CANNED FOOD PRODUCT (CANNED MILK)

The wide array of available dairy foods challenges the microbiologist, engineer, and technologist to find the best ways to prevent the entry of microorganisms, destroy those that do get in along with their enzymes, and prevent the growth and activities of those that escape processing treatment.Troublesome spoilage micro organisms include aerobic psychrotrophic Gram negative bacteria, yeasts, molds, heterofermentative, lactobacilli, and Spore-forming bacteria psychrotrophic extracellular hydrolytic enzymes, and the extend of recontamination of pasteurized fluid milk products with these bacteria is a major determinant of their shelf life. Fungal spoilage of dairy food is manifested by the presence of a wide variety of metabolic by-products, causing off-odors in color or texture. Coliforms, Yeasts, heterofermentative lactic acid bacteria, defects in cheeses. The rate of spoilage of many dairy food is slowed by the application of one or more of the following treatments, of one or more of the following treatments, reducing the Ph by fermenting the lactose to lactic and adding acids or other approved preservatives. The type of spoilage microorganisms differs widely among, dairy foods because of the selective effects of practices followed in production formulation, processing, packaging storage, distribution, and handling (Loralyn H. Ledenbach and Robert T. Marshall 2005).:1.1 AIMTo determine the presence of Micro organism in expired canned milk product and itÒ€ℒs effect in human system.

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