Determination of ascorbic acid (vitamin c) contents in selected vegetables

Project Abstract

The determination of ascorbic acid (vitamin C) content in vegetables is crucial as it is an essential nutrient with antioxidant properties. In this study, selected vegetables were analyzed to quantify the levels of ascorbic acid present. The vegetables chosen for this analysis were spinach, bell peppers, broccoli, and tomatoes, as they are commonly consumed and known to contain varying amounts of ascorbic acid. The ascorbic acid content was determined using a titration method with 2,6-dichlorophenol indophenol (DCPIP) as the titrant. The results indicated that bell peppers had the highest ascorbic acid content among the selected vegetables, followed by broccoli, tomatoes, and spinach. These findings provide valuable information on the ascorbic acid content in commonly consumed vegetables, which can aid in promoting a balanced diet rich in essential nutrients. Further research could focus on analyzing a wider variety of vegetables and exploring the impact of different cooking methods on the ascorbic acid content to help educate the public on maximizing the nutritional benefits of vegetables in daily diets.

Project Overview

<p> </p><ol><li><strong>1.0</strong><strong>INTRODUCTION</strong></li></ol><p>Vitamin C is a water-soluble compound that is essential for life. It is involved in many processes in the human body, including: the production of collagen in the connective tissue; the synthesis of dopamine, non-adrenaline and adrenaline in the nervous system; and the synthesis of carnitine, which is important in the transfer of energy to the cell mitochondria. A deficiency in vitamin C causes scurvy, a disease that affected sailors in the 16th – 18th Centuries. It was discovered that fresh fruit, <strong>e.g. </strong>limes and oranges, or sauerkraut (preserved cabbage) provided the sailors with protection from scurvy.</p><p>In Australia and New Zealand, the recommended daily intake (RDI) of Vitamin C is 60 mg. The Nobel-prize winning scientist, Linus Pauling (1901 — 1994), believed in regular mega doses of vitamin C, but this is still regarded as unorthodox in conventional medicine.</p><p>Vitamin C is often used as an antimicrobial and antioxidant in foodstuffs. It was first isolated in 1928 and in 1932 it was proved to be the agent which prevented scurvy (hence its scientific name of “ascorbic acid”, which literally translates as “anti-scurvy acid”). Its structure was determined in 1933 and confirmed by total synthesis soon after.</p><p>Ascorbic acid is a stable solid that does not react with air, however, it is rapidly oxidized on exposure to air and light when in aqueous solution.</p><p>Ascorbic acid needed for buoyant health, vitality and endurance. It ensures clear skin, a fresh complexion and healthy gums and teeth. This can be achieved, only if individual and animals adhere to soft summer fruits, like black currents, lemon, oranges to mention a few often times. Its mild deficiency results in gums and teeth easily infected. Also poor growth scurvy, in chromic cases and very slow healing of wounds and fractures (Goldsmith, 2001).</p><ol><li><strong>1.1</strong><strong>Structure of </strong><strong>Vitamin C</strong></li></ol><p><strong>CHEMICAL DATA</strong></p><p><strong>Formula</strong><br>C6H806</p><p><strong>Mol. Mass</strong><br>176.12g/mole</p><p><strong>Physical data</strong></p><p><strong>Density</strong><br>1.694g/cm3</p><p><strong>Melt. point</strong><br>1900c (3740F)</p><p><strong>Boiling point</strong><br>5530C (10270F)</p><p><strong>1.2 </strong><strong>Vitamin C the Antioxidant</strong></p><p>In biological systems, reactive oxidants are often produced from metabolic processes. They have the ability to react with other molecules <strong>(e.g. </strong>DNA), thus damaging the cell. The body protects its cells by utilizing another group of molecules called antioxidants (to which vitamin C belongs) to reduce (and hence detoxify) the oxid ants.</p><p><strong>1.3 Volumetric Analysis</strong></p><p>Volumetric analysis is a technique that employs the measurement of volumes to determine quantitatively the amount of a substance in solution. In any reaction between two or more species, the reaction equation shows the stoichiometric ratio of reacting species. Take, for example, the reaction being investigated today, that between solutions of ascorbic acid and 12.</p><p><strong>1.4 Indicators</strong></p><p>12 forms a blue complex in the presence of starch (Vitex) and hence can act as its own indicator. Whilst the ascorbic acid is in excess, 12 which is being added from the burette is being reduced and is decolourising. As soon as all the ascorbic acid present has been oxidised, the added 12 will no longer be reduced and, due to the Vitex that has been added, the solution becomes pale blue.</p><p><strong>1.5 The </strong><strong>Aim of Study</strong></p><p>Cabbage widely contains a reasonable percent of vitamin C (Ascorbic Acid). From the findings made so far, most people take vegetables for pleasure purpose, seldom give a thought about the value and importance of Ascorbic acid in them. This project and research is carried out to determine the vale of vitamin C contains in the selected vegetables.</p><p>Consequently, this study will show and stress the need and desire for consumption of vegetables. Similarly, to elucidate, the need and importance of vegetable and vitamin C, and best possible ways of using and preserving it, ascorbic acid oxidize that would otherwise attack the vitamin C.</p><p><strong>1.6 The </strong><strong>Objectives of the Study</strong></p><p>The objectives of this study are:</p><ol><li>Determine the concentration of vitamin C in selected vegetables</li><li>Ascertain the nutritional status of our vegetable;</li><li>Compare the vitamin C level in vegetables.</li></ol> <br><p></p>