Susceptibilities of salmonella typhi and other bacterial pathogens to antibiotics and hot aqueous extract of hibiscus sabdariffa

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Antibiotic Resistance in Bacterial Pathogens
  • 2.2Mechanisms of Antibiotic Resistance
  • 2.3Hibiscus Sabdariffa and its Medicinal Properties
  • 2.4Previous Studies on Antibiotic Effects of Hibiscus Sabdariffa
  • 2.5Antibacterial Properties of Hibiscus Sabdariffa Extract
  • 2.6Comparative Studies on Antibiotics and Hibiscus Sabdariffa
  • 2.7Effects of Temperature on Antibiotic Sensitivity
  • 2.8Effects of Hibiscus Sabdariffa Extract with Antibiotics
  • 2.9Challenges in Combating Antibiotic Resistance
  • 2.10Future Directions in Antibiotic Research

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Design and Methodology
  • 3.2Selection of Bacterial Pathogens
  • 3.3Antibiotic Susceptibility Testing Methods
  • 3.4Preparation of Hibiscus Sabdariffa Extract
  • 3.5Experimental Setup and Conditions
  • 3.6Data Collection and Analysis Methods
  • 3.7Statistical Analysis Techniques
  • 3.8Ethical Considerations in Research

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Analysis of Antibiotic Susceptibility Results
  • 4.2Evaluation of Hibiscus Sabdariffa Extract Effects
  • 4.3Comparison of Antibiotic and Extract Performance
  • 4.4Interpretation of Temperature Influence
  • 4.5Discussion on Combined Antibiotic-Extract Effects
  • 4.6Implications for Antibiotic Resistance Management
  • 4.7Recommendations for Further Research
  • 4.8Practical Applications of Research Findings

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of Findings
  • 5.2Conclusion
  • 5.3Contributions to Antibiotic Research
  • 5.4Implications for Public Health
  • 5.5Recommendations for Practice
  • 5.6Future Research Directions

Project Abstract

<p> </p><p>The susceptibilities of Salmonella typhi and other pathogens to antibiotics and hot aqueous extract of <em>Hibiscus sabdariffa</em>&nbsp;were investigated using agar diffusion and agar well diffusion methods respectively. <em>Salmonella typhi</em>&nbsp;was sensitive to ampicillin, cetriaxone, ciprofloxacin, gentamycin of ofloxacin and perfloxacin. Nitrofurantoin, ampicillin, clarithomycin and augumentin are resistant. <em>Escherichia coli, Klebsiella spp,</em>&nbsp;and <em>Staphylococcus aureus</em>&nbsp;were sensitive to 50%, 70% and 60% of the antibiotics respectively.</p><p><em>Pseudomonas aeruginosa </em>was resistant to all antibiotics.<em>&nbsp;Hibiscus sabdariffa </em>extract (0.6g in 6ml of sterile distilled water) was active against <em>S. typhi</em>&nbsp;at concentrations of 100mg/ml, 50mg/ml and 25mg/ml (inhibitions zone diameter IZDs = 23mm, 20mm and 16mm respectively). <em>Staphylococcus aureus</em>&nbsp;was susceptible to 100mg/ml, 50mg/ml, 25mg/ml and 12.5mg/ml of the extract with IZDs of 29mm, 18mm, 17mm and 14mm respectively. <em>Klebsiella spp</em>&nbsp;was susceptible to concentrations of 25mg/ml and 12.5mg/ml of the extract with IZDs of 15mm and 10mm respectively. <em>Escherichia coli</em>&nbsp;and <em>Pseudomonas</em><em>aeruginosa </em>were resistant to all the concentrations of<em>&nbsp;H. sabdariffa </em>extract. It is therefore imperative to note that the use of medicinal plants is recommended to the Government and Industry</p> <br><p></p>

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