Detection of fungi species involved in parkiabiglobosa spoilage

Project Abstract

Parkia biglobosa, commonly known as African locust bean, is an important food crop in West Africa, especially in Nigeria, where it is widely consumed and integrated into various traditional dishes. However, the spoilage of Parkia biglobosa by fungi poses a significant challenge to its quality and safety. In this study, we aimed to detect the fungi species involved in the spoilage of Parkia biglobosa using molecular techniques. Samples of spoiled Parkia biglobosa pods were collected from local markets in Nigeria. Fungal isolates were obtained from the spoiled pods and cultured on appropriate media. Genomic DNA was extracted from the fungal isolates, and PCR amplification was performed using universal fungal primers targeting the internal transcribed spacer (ITS) region of the rRNA gene. The PCR products were sequenced, and the obtained sequences were analyzed using bioinformatics tools to identify the fungal species. Our results revealed the presence of various fungi species in the spoiled Parkia biglobosa pods, including Aspergillus flavus, Penicillium citrinum, and Rhizopus stolonifer. Aspergillus flavus is known for producing aflatoxins, which are highly toxic and carcinogenic compounds that can contaminate food products. Penicillium citrinum is a common spoilage fungus that can produce mycotoxins, affecting the safety of food items. Rhizopus stolonifer is a common post-harvest pathogen that causes soft rot in fruits and vegetables. The molecular detection of these fungi species in spoiled Parkia biglobosa provides valuable information for understanding the spoilage mechanisms and developing strategies to prevent fungal contamination in this important food crop. By identifying the specific fungi species involved in the spoilage, targeted interventions can be implemented to control their growth and minimize the risk of mycotoxin contamination in Parkia biglobosa products. Overall, this study highlights the importance of molecular techniques in detecting fungi species responsible for spoilage in food crops like Parkia biglobosa. The findings contribute to the knowledge of fungal diversity in spoiled Parkia biglobosa pods and emphasize the need for effective monitoring and control measures to ensure the safety and quality of this essential food crop.

Project Overview

<p> </p><p><strong>INTRODUCTION</strong></p><p>The seed of locust beans (<em>Parkiabiglobosa</em>) plant found growing in the Savannah Africa provides one of the popular seasonings in African diet. The nutritious and delicious food spice is popularly called “ogiri” in Igbo, “iru” in Yoruba and “dawadawa” in Hausa in Nigeria. It is heavily consumed in Nigeria, Ghana, Sierre Leone and Togo (Odunfa, 1985). It serves as source of protein for most of the people whose protein intake is low due to high cost of animal protein sources.</p><p>The African locust bean tree, <em>Parkiabiglobosa</em>are perennial trees legumes which belongs to the sub-family mimosoideae and family leguminosae (now family fabaceae). They grow in the Savannah region of West Africa up to the southern edge of the Sahel zone 13°N (Campbell-Platte, 1980). The plant occurs in a wide range of Natural Savannah woodlands and ithas the capacity to withstand drought conditions because of its deep tap root system (Nwadiaro<em>et al.,</em>&nbsp;2015). A matured locust bean tree (20-30years) can bear about a ton and above harvested fruits. From experience, the tree can start to bear fruits from 5-7 years after planting (Musa, 1991). The African locust bean tree grows to about 20m in height and has bark evergreen pinnate leaves. Its fruit is a brown leathery pod of about 10-30cm long and contains gummy pulps of an agreeable sweet taste, in which lie a number of seeds. It is important indigenous multipurpose fruit tree. <em>Parkiabiglobosa</em>&nbsp;tree plays vital ecological roles in recycling of nutrients from deep soil, by holding soil particles to prevent soil erosion with the aid of its roots. It also provides shade where it is found (Campbell-Platte, 1980). This tree is protected by peasant farmers and rural dwellers for its many benefits. Its wood is a source of fuel energy. It helps to enrich the soils nutrient.</p><p>The most important use of African locust bean is found in its seed which is a legume, although it has other food and non-food uses especially the seeds which serve as a source of useful ingredients for consumption (Campbell-Platte, 1980). It has been reported that the locust bean is rich protein, carbohydrate, soluble sugars and ascorbic acid. The cotyledon is very nutritious, has less fibre and ash content. The oil content is suitable for consumption since it contains very low acid and iodine contents. The oil has very high saponification and hence would be useful in the soap industry (Alabi<em>et al.,</em>&nbsp;2005). It has also been reported that the husks and pods are good for livestock (Douglass, 1996; Obiazoba, 1998). The locust bean tree is also important in medicinal practices in treatment of aliments such as bronchitis, pneumonia, malaria, diarrhoea and as poison for sore eyes (Farombi, 2003).</p><p>Although microorganisms of all groups including bacteria, protozoa, algae, viruses, fungi together with insects and rodents play significant role in food deterioration, the most active and more versatile organisms that affect locust bean seeds and its products causing spoilage when stored are species of bacteria and fungi (Omafuvbe<em>et al.,</em>&nbsp;2000). They can occur on growing crops as well as harvested commodities leading to damage ranging from rancidity, odour and flavour changes and germ layer destruction (Cutler, 1991). In a study to identify the bacterial and fungal flora of deteriorated and maggot infested samples of fermented locust bean seeds, the isolated fungal species were identified as <em>Aspergillusniger, Aspergillusflavus, Penicillium, Rhizopus</em>&nbsp;and <em>Candida</em>species. <em>Parkiabiglobosa</em>&nbsp;seeds are subject to degradation induced by diverse organisms including fungi which are among the most active microorganisms in these processes (Popoola and Akueshi, 1985). Microorganisms associated with fermented locust bean seeds have been widely studied (Odunfa, 1981; Ikenebomeh<em>et al.,</em>&nbsp;1986; Odunfa and Oyewole, 1986; Ogbadu and Okagbue, 1988). Bacilli and Staphylococci were observed to dominate the fermentation together with a number of fungal species causing deterioration of this especially in storage in Northern Nigeria.</p><p><strong>1.1 &nbsp; &nbsp; &nbsp; AIM</strong></p><ul><li>This study is aimed at DETECTING THE FUNGI SPECIES INVOLVED IN <em>PARKIABIGLOBOSA</em>SPOILAGE.</li></ul><p><strong>1.2 &nbsp; &nbsp; &nbsp; SPECIFIC OBJECTIVES</strong></p><ul><li>To isolate and identify fungi associated with locust beans (<em>Parkiabiglobosa</em>).</li><li>To determine the pH, moisture content and titratable acidity of locust beans.</li></ul> <br><p></p>