The Effect Of Crude Of Aloe Barbadensis On Some Hemostatic Parameters Of Fed On Thermoxidized Palm Oil Diets

Project Abstract

ABSTRACT This study aimed to investigate the effect of crude Aloe barbadensis on some hemostatic parameters of rats fed on thermoxidized palm oil diets. Thirty male albino rats were divided into six groups Group 1 served as the control and was fed with a basal diet, Group 2 was fed with a thermoxidized palm oil diet, Groups 3 and 4 were fed with thermoxidized palm oil diet supplemented with 2.5% and 5% crude Aloe barbadensis, respectively, and Groups 5 and 6 were fed with a basal diet supplemented with 2.5% and 5% crude Aloe barbadensis, respectively. The rats were sacrificed after 56 days of feeding, and blood samples were collected for analysis. Results showed that rats fed with thermoxidized palm oil diet had significantly increased clotting time and reduced platelet count compared to the control group. However, supplementation with crude Aloe barbadensis at 5% significantly reduced the clotting time and increased platelet count in rats on thermoxidized palm oil diets. Furthermore, rats on thermoxidized palm oil diets had significantly higher levels of fibrinogen and D-dimers compared to the control group. Supplementation with crude Aloe barbadensis at 5% significantly reduced the levels of fibrinogen and D-dimers in rats on thermoxidized palm oil diets. Overall, the results suggest that crude Aloe barbadensis supplementation has a positive effect on hemostatic parameters in rats fed with thermoxidized palm oil diets, potentially mitigating the adverse effects of thermoxidized palm oil consumption on blood coagulation and platelet function. Further studies are recommended to explore the underlying mechanisms of Aloe barbadensis on hemostatic parameters and its potential as a functional food ingredient for improving cardiovascular health.

Project Overview

INTRODUCTION AND LITERATURE REVIEW

1.0   Introduction

Aloe barbadensis or aloe Vera is a succulent plant from the family “Liliaceae”, it originated in the African content. The genus has many common names and is often referred to as aloe vera, lily of the desert, burn plant, the plant of immortality, first aim plant, wand of heaven and medicinal plant. The name is derived from the Arabic word “Alloeh” meaning “shining bitter substance”. The Genus contains at least 324 species of herbs, shrubs and ;s (cross white and cross white, 1984). Aloe vera is a perennial with 15-30 fleshy leave up to 0.5m long and 8-Pcrn across the base. Saw like teeth mark the margins- of leaves (Grindlay and Reynolds, 1986). Aloe vera plants withstand high temperatures and long periods drought, due to their ability to store water in their succulent leaves. However, freezing temperatures can damage or kill the plant. Medically and non- medically, aloe vera has been used for several thousands of years in different cultures from ancient Egypt to Greece, Rome to China, India and Africa (crosswhite and crosswhite, 1984; Grindlay and Reynolds, 1986).Aloe Barbadensis

In the first century, C.E, the Greek physician, Dioscorides used aloe Vera for mouth infections, sores, wounds and as purgatives. Egyptians, Assyrians and Mediterranean peoples used the latex primarily and the gel as a purgative. The plant was used by the Arabs, Spaniards, ancient Greeks and persians and is still in use by hunters in Africa to reduce perspiration and body scent.Aloe Barbadensis

In 500 B.C, Egyptians recorded the use of aloe vera in treating burns, parasites and infections. The plant was called. the plant of immortality” by the Egyptians because it can live and even bloom without soil and was given as an offering at the funerals of pharaohs. It was also used in the baths of the Egyptian queens Nefertiti and Cleopatra to keep their skin soft and young (Pamplona Roger, 2001). Today, Egyptians still hang an aloe vera plant over the door of a house to provide a long and fruitful life for its occupants. In   India, the plant is used as cathartic, anthelminthic, emmenagogue and stomachic. Aloe vera latex was used before 1930s in the united states as laxatives ton, 1961; crosswhite and crosswhite, 1984; Grindlay and Reynolds, 1986; Evens 1996).

1.2 Aims and objective s of the study

The aim of this study is to ascertain the effect of crude of aloe barbadensis (aloe vera ) on some hemostatic parameters of fed on thermoxidized palm oil diets. The objective is to ascertain if aloe vera has any effect on hemostatic derangements that may result from thermoxidized palm oil diet.Aloe Barbadensis

1.3   Justification of study

It has been known that aloe vera has anti-inflammatory, laxalive, anti-hypertensive, anticancer, hypoglycaemic fects etc but not much work has been done on its effects on Hemostasis, especially in rats placed on a diet mixedwith thermoxidized palm oil. This research work is therefore aimed at elucidating its effect on Hemostatic parameters ofrats fed on thermoxidized palm oil diets.Aloe Barbadensis

1.4 SCOPE OF THE STUDY

The scope of the study involves measuring bleeding time, clotting time, prothrombin time and platelet count in 5 albino wistar rats fed with pellet mixed with thermoxidised palm oil and also 5 albino wistar rat fed with the same mixed pellet and in addition 0.1ml/100g body weight of refined aloe vera gel orally administered for four weeks (28 days)and comparing the results with control group (5 albino wistar rats) fed only on normal pellet for same period.