MICROBIAL EXAMINATION OF SPOILT AVOCADO FRUIT
Table Of Contents
- Title pageCertificationDedicationAcknowledgementList of TablesList of figuresAbstractCHAPTER ONEIntroduction1.1 Background of study1.2 Statement of problem1.3 Aim and Objectives of the study1.4 Hypothesis1.5 Significance of the study1.6 Limitation of the studyCHAPTER TWOLiterature review2.1 The origin of Avocado fruits2.2 Uses of avocado fruits2.3 Preservation of avocado fruits2.4 Ways avocado fruits can be prone to contamination2.5 Microbial spoilage of avocado fruit2.6 Control of bacterial and other disease of avocado fruits and fruits products2.7 Some biochemical test for bacterial isolatesCHAPTER THREEMaterials and methods3.1 Sample collection3.2 Sterilization of media an material used3.3 Preparation of media3.4 Bacteria and fungi isolate from avocado fruits3.5 Bacterial isolation and identification3.6 Fungal isolation and identification3.7 Lactophenole blue staining technique for fungi isolates3.8 Staining techniques for bacteria isolates grains stain3.9 Biochemical test for identification of bacteria isolatesCHAPTER FOURResults and discussion4.1 Bacterial Examination4.2 Fungi, examinationCHAPTER FIVEConclusion and recommendation5.1 Conclusions5.2 RecommendationReference
Project Abstract
Microbial examination of spoilt Avocado fruits was carried out to obtained the causal organism. A total of 60 avocado from Ogbete Main market and New market Enugu were examined by culturing on nutrient agar and sabour and dextrose agar (SM), for bacteria and fungi causing post – harvest spoilage on the fruits; six bacterial species and six fungal species were isolated. The bacteria include Erwinia SP (66.7%), Streptococcus sp, *25%), E. Coli (18.9%) and staphycoccus sp (33.3%), Pseudomonas sp (20%) and Chromobacterium sp (5%) has the lowest percentage occurrence, Erwinia sp, Pseudomonas sp and chromobacterium sp were found to be absent in the control. The fungi species, includes Aspergillus sp (50%), Pericillium sp (25%), Fusarium sp (16.6%), Rhisopus sp (13.3%), currularia sp (8.3%) and Rhoctorula sp (3.3%), were also collected Avocado fruits from Ogbete main market were more contaminated than the avocado form the new market.Project Overview
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