Effect of different carbon sources on the growth of antimicrobial producing (bacillus species in citrillus vugaris)

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objectives of Study
  • 1.5Limitations of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Carbon Sources
  • 2.2Role of Carbon Sources in Microbial Growth
  • 2.3Types of Carbon Sources
  • 2.4Effects of Different Carbon Sources on Microorganisms
  • 2.5Carbon Sources in Antimicrobial Production
  • 2.6Carbon Sources and Bacillus Species Growth
  • 2.7Impact of Carbon Sources on Citrullus Vulgaris
  • 2.8Carbon Utilization by Microorganisms
  • 2.9Carbon Sources and Antimicrobial Activity
  • 2.10Carbon Sources and Biochemical Pathways

Chapter THREE

SYSTEM DESIGN AND IMPLEMENTATION

  • 3.1Research Methodology Overview
  • 3.2Research Design
  • 3.3Sampling Techniques
  • 3.4Data Collection Methods
  • 3.5Data Analysis Procedures
  • 3.6Experimental Setup
  • 3.7Variables and Controls
  • 3.8Statistical Tools Used

Chapter FOUR

SYSTEM TESTING AND EVALUATION

  • 4.1Analysis of Research Findings
  • 4.2Effects of Different Carbon Sources on Growth
  • 4.3Relationship between Carbon Sources and Antimicrobial Production
  • 4.4Comparison of Bacillus Species Growth Rates
  • 4.5Impact of Carbon Sources on Citrullus Vulgaris
  • 4.6Interpretation of Experimental Results
  • 4.7Discussion on Biochemical Pathways
  • 4.8Implications of Findings

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Conclusion and Summary
  • 5.2Summary of Findings
  • 5.3Achievements of the Study
  • 5.4Recommendations for Future Research
  • 5.5Practical Applications of the Study

Project Abstract

<p> </p><p>Effect of different carbon sources on the growth of antimicrobial producing Bacillus species in ogiri-egusi sold in Ogbeta, Orie, Artisian, Kenyatta, Enugu State were evaluated using standard microbiological and analytical methods. 12 bacteria were isolated and identified such as Bacillus<em>subtilis,</em>(33.33), <em>Bacillus pumulis</em>&nbsp;(41.66),</p><p><em>Bacillus licheniformis </em>(25) were identified based on their colonybiochemical characteristics.The isolates of the highest occurrences is <em>bacillus pumilus</em>. Agar well diffusion method was used to determinethe antimicrobial activity against two pathogenic organisms,</p><p><em>Escherichia</em>&nbsp; &nbsp; <em>coli,</em>&nbsp; &nbsp; &nbsp;<em>Staphylococcus</em>&nbsp; &nbsp; <em>aureus.</em>&nbsp; &nbsp; &nbsp;<em>Bacillus</em>&nbsp; &nbsp; &nbsp;<em>subtilis</em>&nbsp; &nbsp; &nbsp;and</p><p><em>Bacillus pumulis, </em>gave a zone of inhibition<em>&nbsp;Escherichia coli </em>0.6mmand 0.8mm after 24 hours of incubation also carbon sources of 5% and 10% sucrose, Glucose and soluble starch were used to check their different carbon sources on the isolates using the spectrophotometer.5% had the highest optimum growth of 1.400 for glucose 1.545 for sucrose,2.376 for soluble starch which can be added in maximum quantity to a medium to produce antibiotics.</p> <br><p></p>

Project Overview

<p> </p><p><strong>1.0 &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; </strong><strong>INTRODUCTION</strong></p><p>Traditional &nbsp;fermented &nbsp;condiments &nbsp;(OGIRI-EGUSI) &nbsp;based &nbsp;on</p><p>vegetable proteins are consumed by different ethnic groups in Nigeria, have been the pride of culinary traditions for centuries. It is evident that these products have played a major role in the food habits of communities in the rural regions serving not only as nutritious non-meat proteins substitute but also as condiment and flavoring agents in soup.</p><p>Traditional methods of manufacture should take advantage of biotechnological progress to assure reasonable quality and at the same time assure safety of these products. The requirements for a sustainable biotechnological development of Nigerian condiments are discussed in the scope of the microbiology and biochemical changes of the raw materials. Fermented vegetables, proteins have potential food uses as protein supplements and as functional ingredients in the fabricated food (Achi. 2005).</p> <br><p></p>

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