Comparative study of disinfectant efficiency of ethanol, bleach and phenolics against pseudomonas aeruginosa and staphylococcus aureus
Table Of Contents
Chapter ONE
INTRODUCTION
- 1.1Introduction
- 1.2Background of study
- 1.3Problem Statement
- 1.4Objective of study
- 1.5Limitation of study
- 1.6Scope of study
- 1.7Significance of study
- 1.8Structure of the research
- 1.9Definition of terms
Chapter TWO
LITERATURE REVIEW
- 2.1Overview of Disinfectants
- 2.2Ethanol as a Disinfectant
- 2.3Bleach as a Disinfectant
- 2.4Phenolics as a Disinfectant
- 2.5Pseudomonas aeruginosa - Characteristics and Risks
- 2.6Staphylococcus aureus - Characteristics and Risks
- 2.7Comparative Studies on Disinfectant Efficiency
- 2.8Previous Research on Ethanol Disinfection
- 2.9Previous Research on Bleach Disinfection
- 2.10Previous Research on Phenolics Disinfection
Chapter THREE
SYSTEM DESIGN AND IMPLEMENTATION
- 3.1Research Methodology Overview
- 3.2Selection of Disinfectants
- 3.3Culturing and Preparation of Microorganisms
- 3.4Experimental Design and Setup
- 3.5Data Collection Methods
- 3.6Data Analysis Techniques
- 3.7Ethical Considerations
- 3.8Limitations of Research Methodology
Chapter FOUR
SYSTEM TESTING AND EVALUATION
- 4.1Efficiency of Ethanol against Pseudomonas aeruginosa
- 4.2Efficiency of Ethanol against Staphylococcus aureus
- 4.3Efficiency of Bleach against Pseudomonas aeruginosa
- 4.4Efficiency of Bleach against Staphylococcus aureus
- 4.5Efficiency of Phenolics against Pseudomonas aeruginosa
- 4.6Efficiency of Phenolics against Staphylococcus aureus
- 4.7Comparative Analysis of Disinfectants
- 4.8Discussion on Findings
Chapter FIVE
SUMMARY, CONCLUSION AND RECOMMENDATIONS
- 5.1Summary of Findings
- 5.2Conclusion
- 5.3Recommendations for Further Research
- 5.4Implications of the Study
Project Abstract
<p> Ethanol, Bleach and Phenolics are three kinds of disinfectants which have been widely used in common laboratories. In this study, a compared experiment on these three disinfectants efficiency was conducted against <em>Staphylococcus aureus and Pseudomonas aeruginosa</em> using agar hole diffusion method. Different concentrations of bleach (1%, 2%, 3%, 4% and 5%) were used on both organisms. Also (50%, 60%, 70%, 85% and 95%) of ethanol as well as (5%, 10%, 20%, 25%, and 30%) Phenolics were used. Diffrences in concentrations tested was because, the original concentrations of the disinfectants differs. After 24 hours of incubation at 370C, the results showed that all the disinfectants inhibited the growth of the test organism in their concentrated forms. The diameter of zone of inhibitions were measured around each well by using a ruler in millimeters, using different concentrations, their efficacies varied. The results showed that 30% Phenolics had the best efficiency against both test organisms and 5% bleach had a better effect on <em>Staphylococcus aureus</em> than <em>Pseudomonas aeruginosa</em>, while ethanol showed least sensitivity. 70% concentration gave the highest effect on <em>Staphylococcus aureus</em> as compared with<em>Pseudomonas aeruginosa.</em> <br></p>
Project Overview
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</p><p>1.0 INTRODUCTION</p><p> Microorganisms are minute living things that individually are too small to be seen with the unaided eyes (Tortora <em>et al</em>, 2007). Though only a minority of microorganisms are pathogenic (disease producing), practical knowledge of microbes is necessary for medicine and related health sciences. For example hospital workers must be able to protect patients from common microbes that are normally harmless but pose a threat to the sick and injured. Thousands of people died in devastating epidemics; the cause of which was not understood. Entire families died because vaccination and antibiotics were not available to fight infection (Johnson and Case, 1995). This leads to scientific control of microbial growth. This began only about 100 years ago. It was Pasteur’s work on microorganism that led scientists to believe that microbes were a possible cause of diseases and need to be eliminated or destroyed. Some examples off these microbes are; Bacteria, fungi, viruses and protozoa etc (Tortora et al, 2007).</p>
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