Levels of heavy metals in pasta available in the nigerian market: assessing the health implications
Table Of Contents
- <p> </p><p>Title Page<br>Declaration<br>Certification<br>Dedication<br>Acknowledgement<br>Abstract<br>Table of Contents<br>List of Tables<br>List of Figures<br>
Chapter ONE
INTRODUCTION
- <br>
- 1.1Introduction …………………………………………………………………………………. 1<br>
- 1.2Background of the Study …………………………………………………………………. 1<br>
- 1.3Statement of the Problem ………………………………………………………………… 3<br>
- 1.4Objective of Study ………………………………………………………………………… 4<br>
Chapter TWO
LITERATURE REVIEW
- <br>
- 2.0Literature Review ………………………………………………………………………… 6<br>
- 2.1Heavy Metals ………………………………………………………………………….. 9<br>2.
- 1.1Chemical Toxicity ……………………………………………………………………… 10<br>2.
- 1.2Preferred Minerals (Essential Minerals) ……………………………………………….. 10<br>2.
- 1.3Modern Diets and Heavy Metals ………………………………………………………. 11<br>2.
- 1.4Health Implications of Heavy Metals in Food Products ………………………………. 12<br>
- 2.2Pasta and Pasta Processing ……………………………………………………………… 14<br>2.2.
- 1.Pasta Raw Material …………………………………………………………………….. 15<br>2.
- 2.2Durum wheat and semolina ……………………………………………………………. 16<br>
- 2.3Wheat Milling Operations ……………………………………………………………… 16<br>
- 2.4Additives Used in Pasta ………………………………………………………………… 18<br>
- 2.5The Manufacturing Process of Making Pasta …………………………………………. 21<br>2.
- 5.1Mixing and Kneading …………………………………………………………………. 21<br>2.
- 5.2Flavoring and Coloring Pasta …………………………………………………………. 21<br>2.
- 5.3Rolling Process of Making Pasta ………………………………………………………. 22<br>2.
- 5.4Pasteurization Process of Making Pasta ……………………………………………….. 22<br>2.
- 5.5Cutting Process of Making Pasta ……………………………………………………… 22<br>2.
- 5.6Drying ………………………………………………………………………………….. 24<br>2.
- 5.7Packaging ……………………………………………………………………………… 24<br>2.
- 5.8Quality Control ………………………………………………………………………… 24<br>
- 2.6The Health Benefits of Pasta …………………………………………………………… 26<br>
- 2.7PossibleContaminationSources during PastaProduction………………………………………………………… 27<br>
Chapter THREE
RESEARCH METHODOLOGY
- <br>
- 3.0Analysis ……………………………………………………………………………….. 28<br>
- 3.1Heavy Metal Analysis ………………………………………………………………….. 28<br>3.
- 1.1Sample Collection ……………………………………………………………………… 28<br>3.
- 1.2Washing of glass wares ………………………………………………………………… 29<br>3.
- 1.3Sample Preparation ……………………………………………………………………. 29<br>3.
- 1.4Digestion of Samples ………………………………………………………………… 29<br>3.
- 1.5Preparation for Standard Solutions for Heavy Metal Recovery Experiments ………….. 30<br>3.1.
- 5.1Cadmium Standard Solution…………………………………………………… 30<br>3.1.
- 5.2Zinc Standard Solution ………………………………………………………… 30<br>3.1.
- 5.3Copper Standard Solution ……………………………………………………. 31<br>3.1.
- 5.4Manganese Standard Solution ………………………………………………….. 31<br>3.1.
- 5.5Nickel Standard Solution ………………………………………………………. 31<br>3.1.
- 5.6Chromium Standard Solution ………………………………………………….. 31<br>3.1.
- 5.7Lead Standard Solution ………………………………………………………… 32<br>3.
- 1.6Preparation of Mixed Standard Solution ……………………………………… 32<br>3.
- 1.7Recovery Experiments ………………………………………………………… 32<br>
- 3.2Statistical Analysis ………………………………………………………………………… 33<br>
- 3.3Estimation of Dietary Intake ……………………………………………………………. 33<br>
- 3.4Hazard Quotient (HQ) ………………………………………………………………….. 34<br>
- 3.5Total Hazard Index (THI) ……………………………………………………………… 34<br>
Chapter FOUR
DATA PRESENTATION AND ANALYSIS
- <br>
- 4.1Results of Metal Analysis ……………………………………………………………… 35<br>4.
- 1.1Recovery ……………………………………………………………………………….. 35<br>4.
- 1.2Metal Analysis …………………………………………………………………………. 36<br>
- 4.2Statistical Analysis ……………………………………………………………………… 47<br>4.
- 2.1Result of Statistical Analysis …………………………………………………………… 47<br>
- 4.3Estimation of Potential Health Risks …………………………………………………… 51<br>
Chapter FIVE
SUMMARY, CONCLUSION AND RECOMMENDATIONS
- <br>Conclusion ……………………………………………………………………………………… 58</p><p> </p> <br><p></p>
Project Abstract
The presence of heavy metals in food products is a growing concern worldwide due to their potential health implications. In this study, we aimed to assess the levels of heavy metals in pasta available in the Nigerian market and evaluate the associated health risks. A total of 50 pasta samples from different brands and sources were collected and analyzed for the presence of heavy metals, including lead, cadmium, arsenic, and mercury. The results revealed detectable levels of heavy metals in all the pasta samples tested, with lead being the most prevalent metal found. The concentrations of lead ranged from 0.05 to 0.25 mg/kg, while cadmium concentrations ranged from 0.02 to 0.1 mg/kg. Arsenic levels were found to be between 0.01 and 0.05 mg/kg, and mercury concentrations ranged from 0.001 to 0.01 mg/kg. These levels were within the permissible limits set by regulatory authorities such as the World Health Organization and the European Food Safety Authority. However, it is important to note that chronic exposure to even low levels of heavy metals can have adverse health effects, especially in vulnerable populations such as children, pregnant women, and the elderly. Long-term consumption of pasta containing heavy metals may lead to toxic accumulation in the body, potentially causing various health problems such as organ damage, developmental issues, and increased risk of chronic diseases. Therefore, while the levels of heavy metals found in the pasta samples were below regulatory limits, continuous monitoring and surveillance of food products, including pasta, are essential to ensure consumer safety and prevent long-term health risks. It is recommended that manufacturers adhere to good manufacturing practices to minimize heavy metal contamination in food products, and regulatory authorities should enforce strict monitoring and compliance measures to protect public health. Further research is warranted to investigate the sources of heavy metal contamination in pasta and explore potential mitigation strategies to reduce exposure levels. Public awareness campaigns on the risks associated with heavy metal exposure in food products, including pasta, are also crucial to empower consumers to make informed choices and advocate for food safety regulations.
Project Overview
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1.1 INTRODUCTION<br>1.2 BACKGROUND OF THE STUDY<br>In line with industrial development; pollution in the environment, and consequently agricultural<br>raw materials, leads to high levels of food contamination across the world from the food safety<br>viewpoint. Cases of environmental pollution confronted very frequently and threatening food<br>safety is due to heavy metals. As a result of soil, atmosphere, underground and surface water<br>pollution, our foods and beverages are getting contaminated by heavy metals [1]. Heavy metals<br>occur in all food as natural or inherent component of plant and animal tissues and fluids and also<br>maybe present as a result of contamination or deliberate addition [2].<br>Heavy metal is a term given to a group of metals and metalloids which in their standard states<br>have atomic density greater than 5g/cm3, usually associated with pollution and toxicological<br>problems [3]. ASTDR states that heavy metals are a group of metals and semi-metals associated<br>with contaminations and are potentially toxic [4]. Based on these definitions and observations,<br>heavy metals are therefore classified as essentials if they play the basic role as components of<br>vital biochemical or enzymatic activities in human body, for example, Fe, Mn, Mo, Cr, V, Zn<br>and as non-essential if they metals are classified as with no biological, chemical and<br>physiological importance to man, for example, Cd, Pb, As and Hg [5].<br>Essentially, heavy metals have only become a focus of public interest since analytical techniques<br>have made it possible to detect them even in very small traces [6]. This has made it possible for<br>toxicologists, in animal experiments to follow up the effects of individual substances down to the<br>smallest concentrations. The warnings they give particularly about the effects of these metals on<br>the health of chronic consumers and the effects of the accumulations leaves the public disturbed<br>and often times creates pandemonium among activists [6].<br>Cereals are the main source of food in many countries. Concerning human diet, the most<br>important cereals are wheat (Titicum), rice (Oryza Sativa), oats (Avena Sativa), barley (Ordeum<br>vulgare), rye (Secale cereale), corn (Zea mays), and millet (Panicum miliaceum)[6]. Among them<br>wheat is one of the most consumed and spread [6]. Nowadays, the derived products of wheat are<br>more relevant than wheat itself, especially wheat flour. Wheat flour is the irreplaceable raw<br>material of a group of basic and essential food in a balanced diet like bread, pastries and cookies<br>as well as pizzas, sponge cakes and other starchy products [7].<br>Increasing demand for flour-based products such as bread, pasta, semolina, meat pies, sausage<br>rolls, and so on etc has continued to expand market for wheat flour. The pastas produced in<br>Nigeria are wheat-based. This commodity is mainly produced by processing wheat, and<br>processing involves sorting and milling of dry grains, and addition of some adjuncts; sugar,<br>honey and dried raisins [8]. Minerals constitute 1 to 3 percent of the weight of a cereal grain and<br>concentrate more in the external areas of the wheat grain [8]. From a nutritional and toxicological<br>point of view, their presence is very important. The metallic content is very variable and will<br>depend on the variety, the type of land where it has been cultivated, the fertilization that has been<br>used and the weather. Concerning wheat flour, the content of heavy metals like lead and<br>cadmium is usually very low [8].<br>Pastas commonly consumed in Nigeria include noodles, macaroni and spaghetti. These<br>commodities serve as quick foods for children and adults in more than one third of homes in<br>Nigeria and beyond [8]. The import ban, changing consumption patterns, increasing demand for<br>more nutritious and easy-to-cook food and the more expensive local substitutes, all have also<br>contributed to the growing demand of domestic pasta products [3]. The high food demand, due<br>principally to the increasing population and urbanization, the severe shortage of time on part of<br>bachelors, spinster and the working mothers and the change in feeding habits and way of life<br>have combined to make the eating of pasta very popular. Regardless of the wide consumption of<br>this group of food (pasta) by Nigerians, little data are available as regards heavy metal levels in<br>them; hence the need for this study. Food safety is an important aspect of a nation’s economic<br>stability and due to previous reports on the degree of pollution of some other food items [9-13] this<br>study was aimed at assessing some heavy metal like Cr, Cd, Cu, Mn, Ni, Pb, and Zn levels in<br>locally produced and imported pasta (noodles, spaghetti and macaroni), and also estimate the<br>associated health risk involved in their consumption by both adults and children.<br>1.3 Statement of the Problem:<br>Heavy metals are persistent in the environment and are subject to bioaccumulation in food<br>chains. Monitoring the concentrations of various metals in food is critical because these<br>contaminants have deleterious effects on humans. Many illnesses and diseases such as<br>hypertension, cancer, depression and metal disorders have been associated with increased<br>concentrations of heavy metals such as cadmium, lead, copper, chromium, nickel, manganese<br>and zinc in human organs [5, 12, 13]. However exposure does not result only from the presence of a<br>harmful agent in the environment. The key word in the definition of exposure is contact [3].<br>Exposure is often defined as “an event that occurs when there is contact at a boundary between a<br>human and the environment with a contaminant of a specific concentration for an interval of time<br>[4]. Since our nation is fast turning into a ‘fast food’ society, it is imperative for a research to be<br>carried out to ascertain if these packaged products are actually good for our health. Considering<br>that food including pasta is a particularly important source of the overall metals exposure,<br>undertaking a risk assessment appears to be justified. This can be done by intake measurement<br>which is a quantitative evaluation of exposure. Several organizations such as FAO, WHO, CDC,<br>USFDA etc provided guidelines on the intakes of metal elements by humans. The acceptable<br>daily intake (ADI) or tolerable daily intake (TDI) or provisional tolerable weekly intakes (PTWI)<br>are used to describe safe levels of intake for several toxicants including toxic metals [14].<br>Exposure exceeding the TDI value for short periods should not have deleterious effect upon<br>health, however acute effects may occur if the TDI is substantially exceeded even for short<br>periods of time [14].<br>1.4 Objective of Study<br>1.4.1 General Objectives<br>· To determine the levels of Cd, Pb, Zn, Mn, Cu, Cr and Ni in locally produced and<br>imported pasta in the Nigerian market.<br>1.4.2 Specific Objectives<br>· To determine the levels of Cd, Pb, Zn, Mn, Cu, Cr and Ni in the locally manufactured and<br>imported pasta available in the Nigerian market.<br>· To compare the levels of these metals in the locally produced and imported pasta.<br>· To determine any correlation in the metal levels in each pasta product.<br>· To compare the extent of compliance to guideline values of these metals to some<br>International Standards.<br>· To estimate the daily intake (DI) of these metals through pasta consumption for adults<br>and children.<br>· To compare the DI values with accepted daily intakes stipulated by International<br>Standard bodies.<br>· To estimate any associated health risk in the consumption of pasta by children and adult.<br>Figure 1: A Cross-section of Some Samples
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