The effect of sodium citrate and reduced glutathione (gsh) on arginase from human fibriod tissue
Table Of Contents
- <p> </p><p><strong><em>Abstract </em></strong></p><p><strong>
Chapter ONE
INTRODUCTION
- </strong></p><p>
- 1.0 Introduction and Literature Review</p><p>
- 1.1 Fibroid</p><p>1.
- 1.1 Location and classification of fibroid</p><p>1.
- 1.2 Pathogenesis and Pathophysiology</p><p>1.1.
- 2.1 Pathogenesis</p><p>1.1.
- 2.2 Pathophysiology</p><p>1.
- 1.3 Signs, Symptoms and Causes of fibroid</p><p>
- 1.2 Arginase</p><p>1.
- 2.1 Role of arginase in human body</p><p>1.
- 2.2 Aginase activities in Benign tumour</p><p>1.
- 2.3 Arginase II expression in benign and malignant prostate tissue</p><p>
- 1.3 Glutathione (GSH)</p><p>1.
- 3.1 Role of glutathione as an antioxidant</p><p>1.
- 3.2 Glutathione response to exercise</p><p>
- 1.4 Sodium citrate</p><p>
- 1.5 Aims and objectives</p><p>
Chapter TWO
LITERATURE REVIEW
- </p><p>
- 2.0 Materials and Methods</p><p>
- 2.1 Materials</p><p>2.
- 1.1 Apparatus</p><p>
- 2.2 Methods</p><p>2.
- 2.1 Preparation of buffers and reagents</p><p>
- 2.3 Biochemical test</p><p>2.
- 3.1 Arginase assay</p><p>2.
- 3.2 Protein standard curve</p><p>2.
- 3.3 Effect of Glutathione (GSH)</p><p>
Chapter THREE
RESEARCH METHODOLOGY
- </p><p>
- 3.0 Results</p><p>
Chapter FOUR
DATA PRESENTATION AND ANALYSIS
- </p><p>
- 4.0 Discussion, Conclusion and Recommendation</p><p>
- 4.1 Discussion</p><p>
- 4.2 Conclusion</p><p>
- 4.3 Recommendations</p><p>References</p><p>LIST OF FIGURES</p><p>Figure 1 – Uterine Fibroid</p><p>Figure 2 – Hydrolysis of L-arginine</p><p>Figure 3 – Structure of arginase and the active site</p><p>Figure 4 – Showing the effect of sodium citrate on arginase from human fibroid tissue</p><p>Figure 5 – Showing the effect of Glutathione (GSH) on arginase from human fibroid tissue.</p> <br><p></p>
Project Abstract
<p> </p><p>The research is aimed at investigating the effect of sodium citrate and reduced glutathione (GSH) on arginase from human fibriod tissue.</p><p>The research shows that arginase enzyme which was partially purified was found to have a specific interaction with its substrate.</p><p>The study shows that varying the substrate concentration at constant volume of Sodium citrate and Reduced glutathione (GSH) resulted into an Un-competitive inhibition and Competitive inhibition of enzymes respectively.</p><p>The results obtained from this study shows that the inhibition nature of arginase by different inhibitors of enzymes gives a promising approach to abnormal arginase activities in cell proliferation of uterine muscle which leads to fibroid.</p> <br><p></p>
Project Overview