The effect of sodium citrate and reduced glutathione (gsh) on arginase from human fibriod tissue

 

Table Of Contents


  • <p> </p><p><strong><em>Abstract &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</em></strong></p><p><strong>

Chapter ONE

INTRODUCTION

  • </strong></p><p>
  • 1.0&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Introduction and Literature Review</p><p>
  • 1.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Fibroid</p><p>1.
  • 1.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Location and classification of fibroid</p><p>1.
  • 1.2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Pathogenesis and Pathophysiology</p><p>1.1.
  • 2.1&nbsp; &nbsp; &nbsp; Pathogenesis</p><p>1.1.
  • 2.2&nbsp; &nbsp; &nbsp; Pathophysiology</p><p>1.
  • 1.3&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Signs, Symptoms and Causes of fibroid</p><p>
  • 1.2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Arginase</p><p>1.
  • 2.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Role of arginase in human body</p><p>1.
  • 2.2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Aginase activities in Benign tumour</p><p>1.
  • 2.3&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Arginase II expression in benign and malignant prostate tissue</p><p>
  • 1.3&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Glutathione (GSH)</p><p>1.
  • 3.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Role of glutathione as an antioxidant</p><p>1.
  • 3.2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Glutathione response to exercise</p><p>
  • 1.4&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Sodium citrate</p><p>
  • 1.5&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Aims and objectives</p><p>

Chapter TWO

LITERATURE REVIEW

  • </p><p>
  • 2.0&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Materials and Methods</p><p>
  • 2.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Materials</p><p>2.
  • 1.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Apparatus</p><p>
  • 2.2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Methods</p><p>2.
  • 2.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Preparation of buffers and reagents</p><p>
  • 2.3&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Biochemical test</p><p>2.
  • 3.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Arginase assay</p><p>2.
  • 3.2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Protein standard curve</p><p>2.
  • 3.3&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Effect of Glutathione (GSH)</p><p>

Chapter THREE

RESEARCH METHODOLOGY

  • </p><p>
  • 3.0&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Results</p><p>

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • </p><p>
  • 4.0&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Discussion, Conclusion and Recommendation</p><p>
  • 4.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Discussion</p><p>
  • 4.2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Conclusion</p><p>
  • 4.3&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Recommendations</p><p>References</p><p>LIST OF FIGURES</p><p>Figure 1 – &nbsp; &nbsp; Uterine Fibroid</p><p>Figure 2 – &nbsp; Hydrolysis of L-arginine</p><p>Figure 3 – &nbsp; Structure of arginase and the active site</p><p>Figure 4 – &nbsp; Showing the effect of sodium citrate on arginase from human fibroid tissue</p><p>Figure 5 – &nbsp; Showing the effect of Glutathione (GSH) on arginase from human fibroid tissue.</p> <br><p></p>

Project Abstract

<p> </p><p>The research is aimed at investigating the effect of sodium citrate and reduced glutathione (GSH) on arginase from human fibriod tissue.</p><p>The research shows that arginase enzyme which was partially purified was found to have a specific interaction with its substrate.</p><p>The study shows that varying the substrate concentration at constant volume of Sodium citrate and Reduced glutathione (GSH) resulted into an Un-competitive inhibition and Competitive inhibition of enzymes respectively.</p><p>The results obtained from this study shows that the inhibition nature of arginase by different inhibitors of enzymes gives a promising approach to abnormal arginase activities in cell proliferation of uterine muscle which leads to fibroid.</p> <br><p></p>

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