Prevalence of salmonella spp. and e. coli on some automated teller machine (atm) surfaces within kaduna-north local government, kaduna state
Table Of Contents
- <p> </p><div><p>itle page – – – – – – – – – i</p><p>Dedication – – – – – – – – ii</p><p>Declaration – – – – – – – – – iii</p><p>Project Approval – – – – – – – – iv</p><p>Acknowledgement – – – – – – – – v</p><p>Table of content – – – – – – – – vi</p><p>Abstract – – – – – – – – – vi<strong>i</strong></p><p><strong>
Chapter ONE
INTRODUCTION
- </strong></p><p>
- 1.0 Introduction – – – – – – – – 1</p><p>
- 1.1 Aim – – – – – – – – – 4</p><p>
- 1.2 Objectives – – – – – – – – 4</p><p>
- 1.3Statement of Research Problem – – – – – – 4</p><p>
- 1.4 Justification – – – – – – – – 5</p><p><strong>
Chapter TWO
LITERATURE REVIEW
- </strong></p><p>
- 2.0 Literature review- – – – – – – – 6</p><p>
- 2.1 An automated Teller Machine (ATM machine) – – – – 6</p><p>
- 2.2 History and invention of ATM machines – – – – 6</p><p>
- 2.3 ATMs contact surfaces – – – – – – – 7</p><p>
- 2.4 Hygiene – – – – – – – – – 8</p><p>
- 2.5Poor personal hygiene – – – – – – – 8</p><p>
- 2.6 Cross contamination – – – – – – – 9</p><p>
- 2.7 Outbreak associated with ATM contact surfaces- – – – 9</p><p>
- 2.8 Contributing factors to illness- – – – – – – 10</p><p>
- 2.9Enteric Bacteria – – – – – – – – 13</p><p>2.
- 1.1Selected genera of the Family <em>Enterobacteriaceae</em> – – – 16</p><p>2.1.2<em>Escherichia coli</em>– – – – – – – – – 16 </p><p>2.
- 1.3<em>Salmonella species.</em> – – – – – – – 21</p><p><strong>
Chapter THREE
RESEARCH METHODOLOGY
- </strong></p><p>
- 3.0Materials and methods – – – – – – – 23</p><p>
- 3.1Sampling sites – – – – – – – – 23</p><p>
- 3.2 Sample collection – – – – – – – 23</p><p>
- 3.3 Media preparation – – – – – – – 24</p><p>
- 3.4 Bacteriological Procedures – – – – – – 24</p><p>3.
- 4.1 Serial dilution – – – – – – – – 24</p><p>3.
- 4.2 Inoculation and incubation of samples – – – – 25</p><p>3.
- 4.3Gram staining – – – – – – – – 26</p><p>
- 3.5 Biochemical tests – – – – – – – 27</p><p>3.
- 5.1 Citrate utilization test – – – – – – – 27</p><p>3.
- 5.2 Indole test – – – – – – – – 27</p><p>3.
- 5.3 Methyl red test – – – – – – – – 27</p><p>3.
- 5.4 Voges-Proskauer test – – – – – – – 28</p><p>3.
- 5.5 Triple sugar iron test – – – – – – – 28</p><p>3.
- 5.6 Urease test – – – – – – – – 28</p><p><strong>
Chapter FOUR
DATA PRESENTATION AND ANALYSIS
- </strong></p><p>
- 4.0 Results – – – – – – – – – 30</p></div><h3></h3><br> <br><p></p>
Project Abstract
Automated Teller Machines (ATMs) have become ubiquitous in society, providing convenient access to financial services. However, these machines are frequently touched by numerous individuals, raising concerns about the potential for contamination with pathogenic microorganisms. In this study, we investigated the prevalence of Salmonella spp. and E. coli on ATM surfaces within Kaduna-North Local Government, Kaduna State. A total of 50 ATM surfaces were sampled using sterile swabs and transported to the laboratory for analysis. Microbiological culture techniques were employed to isolate and identify Salmonella spp. and E. coli. Additionally, antibiotic susceptibility testing was performed to determine the resistance patterns of the isolates. The results revealed that 30% of the sampled ATM surfaces were contaminated with Salmonella spp., while 20% were positive for E. coli. The presence of these pathogenic bacteria on ATM surfaces highlights the potential risk of infectious disease transmission through fomites. Furthermore, antibiotic susceptibility testing indicated varying degrees of resistance among the isolates, emphasizing the importance of monitoring antimicrobial resistance in environmental bacteria. Overall, the findings of this study underscore the need for regular cleaning and disinfection of ATM surfaces to minimize the transmission of pathogenic microorganisms. Public health awareness campaigns should also be implemented to educate individuals about the importance of hand hygiene after using ATMs to prevent the spread of infectious diseases. Further research is warranted to explore other potential sources of microbial contamination in public settings and to develop effective strategies for mitigating the risks associated with pathogen transmission via fomites.
Project Overview