Phytochemical screening of ethanol leaves extract of jatropha tanjorensis and its effects on the liver function of male albino wistar rats

Project Abstract

Jatropha tanjorensis is a plant species known for its traditional medicinal uses in various parts of the world. In this study, the ethanol leaves extract of Jatropha tanjorensis was subjected to phytochemical screening to identify the presence of bioactive compounds. The effects of the extract on the liver function of male albino Wistar rats were also investigated. Phytochemical screening revealed the presence of various secondary metabolites such as alkaloids, flavonoids, tannins, saponins, and glycosides in the extract. The oral administration of the extract at different doses to the experimental rats for a specified duration resulted in alterations in various liver function parameters. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin were measured to assess liver function. The results showed dose-dependent effects of the extract on these parameters, indicating a potential impact on liver health. Histopathological examination of liver tissues further supported the biochemical findings, revealing structural changes indicative of liver damage in the extract-treated rats. These findings suggest that the ethanol leaves extract of Jatropha tanjorensis contains bioactive compounds that can affect liver function in male albino Wistar rats. Further studies are warranted to elucidate the specific compounds responsible for the observed effects and to determine the underlying mechanisms of action. Understanding the potential hepatotoxicity of this plant extract is crucial for evaluating its safety and exploring its therapeutic potential in liver-related conditions. Additionally, investigating the dose-response relationship and exploring different administration routes can provide valuable insights for future research and potential clinical applications. This study contributes to the growing body of knowledge on the pharmacological properties of Jatropha tanjorensis and highlights the importance of assessing the safety profile of herbal preparations before their use in traditional medicine or drug development.

Project Overview

<p> </p><p><strong>INTRODUCTION</strong></p><p><strong>1.1 </strong><strong>&nbsp; &nbsp;BACKGROUND INFORMATION</strong></p><p>Plants have been used sinced the medieval times for medicinal purposes for the treatment of all kinds of ailments. Some plants apart from serving as food have also been known to exhibit medicinal properties. Over 400,000 species of tropical flowering plants have medicnal properties. (Akpulu <em>et al,</em>&nbsp;1994). Also about 25% of the drugs prescribed worldwide and found in mordern pharmacopoeias come from plants, 121 such active compounds being in current use. Of 252 drugs considered as basic and essential by the World Health Organisation (WHO,2001), it has been discovered that a total of about 11% are exclusively of plant origin with a large number being synthetic drugs obtained from natural precursors. Some important drug examples obtained from plants are Digitoxin from plant <em>Digtalis spp</em>&nbsp;used in the treatment of heart failures, Quinine used as antimalarials and Qunidine used as antiarythmic are both obtained from plant <em>Cinchona spp</em>, vincristine and vinblastine gotten fron <em>Catharanthus roseus</em>&nbsp;used as anticanceer agents, Atropine from <em>Atropa belladonna</em>&nbsp;and morphine and codeine from <em>Papaver somniferum</em>. An estimated 60% of antitumor and anti-infectious drugs already in the market or under clinical trials are of natural origins. (Sofowora, 1993).</p><p>According to a research by World Health Organisation in 2001 an estimated 80% of the population of developing countries in Africa and Asia are known to rely on traditional medicine, mostly plant drugs for their primary health care needs. (WHO, 2001). Due to extraction and devlopment of several drugs and chemotherapeutics from these plants as well as from traditionally used herbal remedies, there has recently been an increasing reliance on the utilization of medicinal plants in the industrialized societies. The medicinal properties of these plants are suspected to be as a result of the antioxidant, antipyretic, antimicrobial effects of secondary metabolitesof phytochemicals in them. (Soetan and Aiyelaagbe, 2009). Due to reasons such as ineffiency of conventional medicine (e.g side effects of ineffective therapy abuse and incorrect use of synthetic drugs resulting in side effects and other problems) lack of access to conventional pharmacological treatment perhaps due to inaccessibility of health care centres and the ever increasing cost of synthetic drugs, there is a general resurgence of interest in medicinal plants and traditional medicines as a whole by a large percentage of the worlds population. Also folk medicine and ecological awareness suggest that natural products are harmless. Although plant based natural medicines are popularly acclaimed to be safe, scientist advocate for proper toxicological studies (Oyewole <em>et al</em>, 2008), in order to ensure safety in the use of natural medicines. Toxicity is the undesirabe property of any drug or chemical capable of producing injurious or detrimental effects on a living organism. Whether or not these injuries occur depend on the amount of chemical absorbed.(Gossel and Bricker, 1990; Betram,1998). The toxic effect caused by drugs is similar in man and other animals, hence the use of animal models in toxicological studies. Most toxic effects of drugs occur at a predictable time after administration. However, the target organ of toxicity is not neccesarily the site of accumulation.(Oboh, 2009). This study however does not include doses that can induce toxicity upon administration.</p><p><em>Jatropha tanjorensis</em>&nbsp;belongs to the family Euphorbiaceae. It is a common weed of field crops, bush regrowth, road sides and disturbed places in higher rainfall forest zones of West Africa. It is commonly called Hospital Too Far, Catholic Vegetable(Iwalewa <em>et al</em>&nbsp;2005). The leaf is commonly consumed in many parts of Southern Nigeria as it does not require any special conditions to grow in the Southern soil. It is popular as a medicinal plant and is commonly used as a natural remedy for diabetes, malaria infection hypertension and some other conditions in this region.(Olayiwola <em>et al, </em>2004). Also acclaimed as a booster of of blood and immune system; however there is a dearth in th scientific validation of these claims.</p><p>Phytochemical screening of <em>Jatropha tanjorensis </em>leaf revealed that it contains bioactive principles such as alkaloids, flavonoids, tannins, cardiac glycosides, anthraquinone and saponins (Ehimwenma and Osogie, 2007).</p><p>The liver functions as an essential organ making proteins and blood clotting factors, manufacturing triglycerols and cholesterol, glycogen synthesis and bile production. The effect of <em>Jatropha tanjorensis</em>&nbsp;on the liver function of the exprimental animals is determined by testing for liver enzymes suchs as Alkaline phosphatase (ALP), Alanine amino transferase (ALT), Aspartate amino transferase (AST). &nbsp;</p><p>In this study, I investigate the phytochemical composition of <em>Jatropha tanjorensis</em>&nbsp;leaf and also investigate the effect of ethanol extract of the leaf of this plant on the liver function of male albino whistar rats. &nbsp;</p><p><strong>1.2 &nbsp; STATEMENT OF THE PROBLEM</strong></p><p><em>Jatropha tanjorensis</em>&nbsp;is commonly consumed as vegetable and used as a medicinal plant in many parts of Nigeria and has shown hematological, antimalarial, antimicrobial, hypoglycemic, hypolipidemic and antihypertensive activities.(Abiodun <em>et al</em>, 2013). A plant with so many abilities is bound to have &nbsp; tremendous positive or negative effects on the body. The importance of these effects on the liver function cannot be over emphasized as the liver is a vital organ of the body. A situation where <em>Jatropha tanjorensis</em>&nbsp;exudes a positive effect on the liver must be studied as carefully as a situation where the effect is negative or toxic.</p><p><strong>1.3 &nbsp; &nbsp; AIMS AND OBJECTIVES OF THE STUDY</strong></p><p>The aim and objectives of this study is to determine the phytochemical constituent of the ethanolic leaf extract of <em>Jatropha tanjorensis </em>and also determine the effects of these extracts on the liver function of albino whistar rats.</p><p><strong>1.4 </strong><strong>&nbsp; &nbsp; JUSTIFICATION</strong></p><p>In many developing countries such as Nigeria herbal remedies of <em>Jatropha tanjorensis </em>are often relied upon to solve problems and cure diseases. There is therefore need to establish the effect and the promising potential of herbal products such as the leaf extract of this plant on the liver function.</p><p><strong>1.5 </strong><strong>&nbsp; &nbsp;SIGNIFICANCE OF STUDY</strong></p><p>The results from this study will add knowledge to existing literature about the effects of ethanol leaf extracts of our plant of interest on the liver function of albino whistar rats. This data will be useful as a guide to those that make use of this plant as food or as a medicinal herb to now appreciate and be mindful of the effects of the leaf of this plant on liver function. Using this research work, the effective doses of extracts of this plant can be obtained as it pertains to human.</p><p><strong>1.6 </strong><strong>&nbsp; &nbsp; HYPOTHESIS</strong></p><p>H1: &nbsp; <em>Jatropha tanjorensis</em>&nbsp;leaves contains phytochemical compound and have effect on liver function.</p><p>H0: &nbsp; Jatropha tanjorensis leaves do not contain phytochemical compound and have no effect on liver function.</p> <br><p></p>