Partial purification and effect of temperature and heat stability studies on rhodanese from the liver of a local goat

• <p> </p><p><strong>

Chapter ONE

INTRODUCTION

• </strong></p><p>1.1<em>OcimumGratissimum</em></p><p>
• 1.2Phytochemical</p><p>
• 1.3Classes of phytochemicals</p><p>1.
• 3.1Alkaloids</p><p>1.
• 3.2Flavonoids</p><p>1.
• 3.3Saponin</p><p>1.
• 3.4Tannin</p><p>1.
• 3.5Terpenes</p><p>1.
• 3.6Phenolics</p><p>
• 1.4&nbsp; &nbsp; Mechanism of action of phytochemicals</p><p>1.
• 4.1Anti- oxidant</p><p><strong>

Chapter TWO

LITERATURE REVIEW

• </strong></p><p>
• 2.0&nbsp; &nbsp; MATERIALS AND METHOD</p><p>
• 2.1&nbsp; &nbsp; Plant material collection and identification</p><p>
• 2.2&nbsp; &nbsp; Apparatus used</p><p>
• 2.3&nbsp; &nbsp; Chemicals and reagents</p><p>
• 2.4&nbsp; &nbsp; Preparation of plant extract</p><p>
• 2.5&nbsp; &nbsp; Qualitative phytochemical screening</p><p>2.
• 5.1Test for Alkaloids(Wagner’s test)</p><p>2.
• 5.2Test for Saponin (Froth test)</p><p>2.
• 5.3Test for Tannin</p><p>2.
• 5.4Test for Flavonoids</p><p>2.
• 5.5Test for Phytosterols</p><p>2.
• 5.6Test for Phenols</p><p>
• 2.6&nbsp; &nbsp; Quantitative analysis and Anti-oxidant activity</p><p>of O.gratissimum</p><p>2.
• 6.1Estimation of total flavonoids</p><p>2.6.
• 1.1Preparation of sample</p><p>2.6.1.2Preparation of quercetinstandard calibration curve</p><p>2.
• 6.2Determination of tannins concentration</p><p>2.6.2.1Preparation of sample</p><p>2.6.2.2Preparation of tannic acid standard calibration curve</p><p>2.
• 6.3Determination of total Phenol content</p><p>2.6.3.1Preparation of sample</p><p>2.6.3.2Preparation of gallic acid standard</p><p>2.
• 6.4Determination of Saponin concentration</p><p>2.
• 6.5Determination of Alkaloid concentration</p><p>2.
• 6.6Ferric Reducing Power Assay</p><p>
• 2.8&nbsp; &nbsp; Gas Chromatography- Mass Spectrometry</p><p><strong>

Chapter THREE

RESEARCH METHODOLOGY

• </strong></p><p>
• 3.0&nbsp; &nbsp; RESULTS &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</p><p>
• 3.1&nbsp; &nbsp; Qualitative phytochemical screening of <em>O.gratissimum </em></p><p>
• 3.2&nbsp; &nbsp; Quantitative/anti-oxidant analysis of <em>O. Gratissimum &nbsp;</em></p><p>3.
• 2.1Total flavonoid content</p><p>3.
• 2.2Total tannin content</p><p>3.
• 2.3Total Phenol content</p><p>3.
• 2.4Total saponin content</p><p>3.
• 2.5Total alkaloid content</p><p>3.
• 1.3Reducing power assay</p><p>
• 3.2&nbsp; &nbsp; GC-MS analysis</p><p><strong>

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

• </strong></p><p>
• 4.0&nbsp; &nbsp; Discussion, conclusion and recommendation</p><p>
• 4.1&nbsp; &nbsp; Discussion</p><p>
• 4.2&nbsp; &nbsp; Conclusion</p><p>
• 4.3&nbsp; &nbsp; Recommendation</p><p><strong>REFERENCES</strong>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</p><p>APPENDIX.</p> <br><p></p>