Isolation, identification and antibiogram of staphylococcus aureus isolated from cow meat

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of study
  • 1.3Problem Statement
  • 1.4Objective of study
  • 1.5Limitation of study
  • 1.6Scope of study
  • 1.7Significance of study
  • 1.8Structure of the research
  • 1.9Definition of terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Staphylococcus aureus
  • 2.2History of Staphylococcus aureus research
  • 2.3Pathogenicity of Staphylococcus aureus
  • 2.4Transmission of Staphylococcus aureus
  • 2.5Antibiotic resistance in Staphylococcus aureus
  • 2.6Methods for isolation of Staphylococcus aureus
  • 2.7Identification techniques of Staphylococcus aureus
  • 2.8Role of Staphylococcus aureus in foodborne illnesses
  • 2.9Antimicrobial susceptibility testing of Staphylococcus aureus
  • 2.10Current research trends in Staphylococcus aureus

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research design and rationale
  • 3.2Sampling methods
  • 3.3Data collection procedures
  • 3.4Data analysis techniques
  • 3.5Quality control measures
  • 3.6Ethical considerations
  • 3.7Research limitations
  • 3.8Research validity and reliability

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Overview of research findings
  • 4.2Isolation of Staphylococcus aureus from cow meat
  • 4.3Identification of Staphylococcus aureus isolates
  • 4.4Antibiogram analysis of Staphylococcus aureus
  • 4.5Comparison with existing literature
  • 4.6Discussion on antibiotic resistance patterns
  • 4.7Implications of findings on public health
  • 4.8Recommendations for future research

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of research findings
  • 5.2Conclusion of the study
  • 5.3Contributions to the field
  • 5.4Practical implications
  • 5.5Recommendations for stakeholders

Project Abstract

<p> The study was carried out with aim to isolate Staphylococcus aureus from cow meat and determine the antibiogram pattern of S. aureus. Three samples of cow meat from three different locations (Gwagwalada market, Abattoir market and Kasuan Dare) in Gwagwalada were collected to isolate S. aureus from the samples. The organisms isolated were Salmonella spp, Escherichia coli, Staphylococcus aureus and Listeria monocytogenes. Several biochemical tests were carried out to identify, the S. aureus isolates like Catalse test (positive), Manitol test (positive), Coagulase test (positive) and Oxidase test (negative). The antibiogram pattern of S. aureus was evaluated using commercially prepared disk. The antibiotics tested were Pefloxacin (Pef), Streptomycin (S), Ampiclox (Apx), Amoxacilin (Am) and Ciprofloxacin. The pattern indicated that the overall S. aureus were resistant to Pef, S, Cpx and less resistant to Am and Apx. Results clearly suggested a possibility of potential public health threat of S. aureus resulting from contamination of cow meat with pathogenic bacteria which is mainly due to unhygienic processing, handling, and unhygienic environment. <br></p>

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