Isolation, characterisation and antimicrobial activity of bioactive constituents from the leaf extract of adenodolichos paniculatus (hua) hutch. & dalz (fabaceae)

Project Abstract

Adenodolichos paniculatus (Hua) Hutch. & Dalz, a plant belonging to the Fabaceae family, has been of interest due to its potential bioactive constituents. In this study, the leaf extract of A. paniculatus was isolated and characterized to identify its bioactive compounds and assess their antimicrobial activity. The extraction process involved the use of solvents to obtain the bioactive constituents from the leaves of the plant. Characterization techniques such as spectroscopic analysis, chromatography, and mass spectrometry were employed to identify the chemical composition of the extract. The bioactive constituents isolated from the leaf extract were found to exhibit antimicrobial properties against a range of pathogens, including bacteria and fungi. The antimicrobial activity was assessed using various microbiological assays to determine the minimum inhibitory concentration (MIC) and zone of inhibition of the bioactive compounds. The results indicated that the leaf extract of A. paniculatus contains potent antimicrobial compounds that could be further explored for their therapeutic potential. Overall, this study provides valuable insights into the isolation, characterization, and antimicrobial activity of bioactive constituents from the leaf extract of A. paniculatus. The findings suggest that A. paniculatus has the potential to be a source of novel antimicrobial agents that could be developed for pharmaceutical applications. Further research is warranted to explore the specific mechanisms of action of the bioactive compounds and their potential synergistic effects in combination with existing antimicrobial agents.

Project Overview

<p> </p><div><div><div><div><div><div><div><div><div><div><p>The plant <em>Adenodolichos paniculatus</em>&nbsp;which is used in ethno – medicin </p><div><div><div><div><div><div><div><div><div><div><p>The plant <em>Adenodolichos paniculatus</em>&nbsp;which is used in ethno – medicine for the treatment of blennorrhoea, diarrhea, dysentery, small pox, heart burn and wound dressing was investigated for its chemical constituents and antimicrobial activity. The leaves of this plant were subjected to microwave assisted extraction to obtain <em>n</em>&nbsp;– hexane, chloroform, ethyl acetate and methanol extracts. The extracts were then subjected to preliminary phytochemical screening which revealed the presence of alkaloids, anthraquinones, flavonoids, reducing sugars, saponins, tannins and steroids/terpenes. Purification through various column chromatography, the ethyl acetate extract led to the isolation of compounds X1 (mixture of stigmasterol and β – sitosterol) and X2 (nonanoic acid) after spectral analysis IR, 1H NMR and 13C NMR. Determination of inhibitory activity (sensitivity test) using agar well diffusion method showed activity i.e. minimum inhibitory concentration (MIC) against <em>Staphylococcus aureus, Salmonella typhi,</em>&nbsp;and <em>Candida albicans</em>&nbsp;ranging from 12 – 50 mg/ml, Minimum Bactericidal Concentration/ Minimum Fungicidal Concentration (MBC/MFC) ranging from 25 – 50 mg/ml, which is found to be bacteriostatic against <em>Salmonella typhi</em>. <em>Escherichia coli, Pseudomonas aeruginosa,</em>&nbsp;<em>Salmonella typhi, Bacillus subtili </em>showed no MBC (Bacteriostatic) for the methanol, ethyl acetate, chloroform and <em>n</em>&nbsp;–hexane extract.</p><p></p></div><h3><br></h3></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div><p></p>