Isolation and identification of microoragnisms in cassava mill effluent in the soil

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Microorganisms
  • 2.2Importance of Microorganisms in the Environment
  • 2.3Microorganisms in Cassava Mill Effluent
  • 2.4Effects of Microorganisms in the Soil
  • 2.5Methods of Isolating Microorganisms
  • 2.6Identification Techniques of Microorganisms
  • 2.7Role of Microorganisms in Bioremediation
  • 2.8Impact of Microorganisms on Soil Health
  • 2.9Microbial Diversity in Soil
  • 2.10Future Research Directions

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Design
  • 3.2Sampling Techniques
  • 3.3Data Collection Methods
  • 3.4Data Analysis Procedures
  • 3.5Experimental Setup
  • 3.6Quality Control Measures
  • 3.7Ethical Considerations
  • 3.8Statistical Analysis Methods

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Overview of Findings
  • 4.2Isolation and Identification Results
  • 4.3Microbial Community Composition
  • 4.4Physicochemical Properties of Soil
  • 4.5Comparative Analysis of Microorganisms
  • 4.6Discussion on Microbial Interactions
  • 4.7Implications for Soil Health
  • 4.8Recommendations for Future Research

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of Findings
  • 5.2Conclusion
  • 5.3Contributions to the Field
  • 5.4Practical Implications
  • 5.5Limitations of the Study
  • 5.6Recommendations for Further Research

Project Abstract

<p> The Isolation and identification of microorganisms in cassava mill effluent in the soil in Orji, Imo State, Nigeria were carried out, soil samples were collected from site polluted with cassava mill effluent and also from adjacent site not polluted with cassava effluent to serve as control. The overall population of microorganisms in cassava mill effluent was determined. Isolation and characterization of succession of microorganism in cassava mill effluent was studied microbial count, Bacterial counts and fungal counts were also determined using a pour plate method. The microbial count ranges from 2.2 X 105 – 2.0 X 105 for THBC, 1.1 X 105 – 1.2 X 105 for TCC and the TFC ranges from 1.2 X 105-1.5 X 105. The bacteria isolated were <em>Klebsiella spp., Escherichia coli</em>&nbsp;, <em>Pseudomonas spp., Staphylococcus aureus, and Streptococcus spp. </em>while the fugal isolates include <em>Penicillium spp., Aspergillus spp., mucor spp., and </em>yeast cells <em>Candida spp. </em>the cassava mill effluent should be treated before discharge into the environment to prevent pollution. <br></p>

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