Evaluation of the chemopreventive effects of balanite aegypticaand ziziphus spina-christy supplementon n-methyl-n-nitrosourea induced colon carcinogenesis in male wistar rats

Project Abstract

<p> The colon carcinogenesis chemopreventive potential of different levels of <em>Balanites aegyptica</em>&nbsp;and <em>Ziziphus spina-christy</em>&nbsp;diets supplemented at 2.5%, 5% and 10% were examined on N-Methyl-N-Nitrosourea induced colon carcinogenesis in male wistar rats. Following ten (10) weeks of induction with N-Methyl-N-Nitrosourea (MNU) and concomitant feeding with supplemented diets, it was observed that groups on diet supplemented with <em>Balanites</em>&nbsp;<em>aegyptica</em>and<em>&nbsp;Ziziphus spina-christy</em>showed a significant (p˂0.05) decrease in carcinoembryonic antigen (CEA) levels in a dose-dependent manner. There was a significant (p˂0.05) increase in the body weight of the MNU treated with 10 % <em>Balanites aegyptica</em>and <em>Ziziphus spina-christy</em>&nbsp;supplemented diet group. The feeding rate among the treatment groups showed no significant (p˂0.05) difference. Furthermore, there was no significant (<em>p</em>˂0.05) difference in white blood cell, red blood cell counts, hemoglobin concentration and platelets. The MNU treated group that the diet was supplemented with the 10% <em>Balanites aegyptica</em>and <em>Ziziphus spina-christy</em>&nbsp;showed significant increase in superoxide dismutase and catalase activity, while the photomicrograph of the colon of MNU control showed hyperchromiticity, atrophy of gland and necrosis compared to normal mucosal features observed in the normal control with normal colon features. These findings suggest that <em>Balanites aegyptica</em>and <em>Ziziphus spina-christy</em>&nbsp;especially at 10% supplementation level were able to provide a chemopreventive effect against colon carcinogenesis, probably because of their fiber, polyphenol and flavonoid contents. <br></p>

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