Antioxidant activity, total phenolic and flavonoid contents of whole plant methanol extract and solvent fractions of desmodiumramosissimum g. don
Table Of Contents
Chapter ONE
INTRODUCTION
- 1.1Introduction
- 1.2Background of Study
- 1.3Problem Statement
- 1.4Objective of Study
- 1.5Limitation of Study
- 1.6Scope of Study
- 1.7Significance of Study
- 1.8Structure of the Research
- 1.9Definition of Terms
Chapter TWO
LITERATURE REVIEW
- 2.1Overview of Antioxidant Activity
- 2.2Concept of Total Phenolic Contents
- 2.3Importance of Flavonoid Contents
- 2.4Previous Studies on Desmodiumramosissimum G. Don
- 2.5Effects of Methanol Extraction on Plant Properties
- 2.6Solvent Fractionation Techniques
- 2.7Role of Phenolic Compounds in Plants
- 2.8Flavonoids and Their Health Benefits
- 2.9Relationship Between Antioxidants and Health
- 2.10Comparison of Different Extraction Methods
Chapter THREE
RESEARCH METHODOLOGY
- 3.1Research Methodology Overview
- 3.2Selection of Desmodiumramosissimum G. Don
- 3.3Extraction Process Details
- 3.4Fractionation Techniques Applied
- 3.5Analysis of Total Phenolic Content
- 3.6Evaluation of Flavonoid Content
- 3.7Experimental Design and Variables
- 3.8Data Collection and Analysis Methods
Chapter FOUR
DATA PRESENTATION AND ANALYSIS
- 4.1Analysis of Antioxidant Activity Results
- 4.2Comparison of Phenolic Contents
- 4.3Flavonoid Content Variation in Fractions
- 4.4Interpretation of Extraction Efficiency
- 4.5Discussion on Health Implications
- 4.6Comparison with Previous Findings
- 4.7Impact of Solvent Choice
- 4.8Influence of Plant Part on Extract Composition
Chapter FIVE
SUMMARY, CONCLUSION AND RECOMMENDATIONS
- 5.1Summary of Research Findings
- 5.2Conclusion and Recommendations
- 5.3Implications for Future Research
- 5.4Practical Applications of Study
- 5.5Reflection on Study Limitations
Project Abstract
<p> The present study was designed to estimate the total phenolic and flavonoid contents and to evaluate the in vitro antioxidant activities of both the methanol extract and the various solvent fractions of the whole plant, Desmodiumramosissimum. Qualitative phytochemical screening showed the presence of various bioactive components such as phenolics, flavonoids and tannins. The quantitative analyses of total phenolic content (TPC) and total flavonoid content (TFC) were carried out spectrophotometrically using Folin-Ciocalteu phenol reagent and aluminum chloride method, respectively. Ethyl acetate fraction followed by n-butanol fraction exhibited high total antioxidant activity, DPPH radical scavenging ability as well as ferric ion reducing ability when compared to all other solvent fractions including the methanol extract. The presence of these flavonoid and phenolic compounds might contribute to the antioxidant properties observed in these fractions. In order to support this observation, the relationship between the antioxidant content using various models revealed that TPC exhibited a strong correlation with ferric ion reducing potential (FIRP) and total antioxidant capacity (TAC) with R2 vmalues of 0.995 and 0.980, respectively; but a weak relationship with 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical with R2 value of 0.877. However, aqueous fraction showed a strong relationship between DPPH and its concentration compared to other fractions. This showed that various solvent extracts possess unique chemical constituent of plant with various antioxidant capacity. Free radicals were scavenged by the extract and solvent fractions in a concentration-dependent manner within the range of the given concentrations (25-1000 mg/l) in all models. The findings from this study show that methanol extract and the solvent fractions of Desmodiumramosissimum G. Don contained phenolics, flavonoids and tannins which exhibited antioxidant properties. <br></p>Project Overview
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