Activities of na+ k+-atpase in the liver of normal and protein undernourished rats fed with diet containing coconut oil

 

Table Of Contents


  • <p> </p><div><p><strong>

Chapter ONE

INTRODUCTION

  • </strong></p><p>
  • 1.0&nbsp; &nbsp; Introduction</p><p>
  • 1.1&nbsp; &nbsp; Aim of Study</p><p>

Chapter TWO

LITERATURE REVIEW

  • </p><p>
  • 2.0&nbsp; &nbsp; Literature review</p><p>
  • 2.1&nbsp; &nbsp; Coconut oil</p><p>2.
  • 1.1Health benefits</p><p>
  • 2.2&nbsp; &nbsp; Polysaturated fatty acid</p><p>
  • 2.3&nbsp; &nbsp; Respiratory Electron Transport Chain</p><p>
  • 2.4&nbsp; &nbsp; Sodium-Potassium ATPase (Na+– K+ATPase)</p><p>
  • 2.5&nbsp; &nbsp; Mitochondria</p><p>
  • 2.6&nbsp; &nbsp; Protein Undernutrition</p><p>2.
  • 6.1Mild and moderate malnutrition</p><p>
  • 2.7&nbsp; &nbsp; Liver</p><p>2.
  • 7.1Synthesis</p><p>2.
  • 7.2&nbsp; &nbsp; Liver disease</p><p>2.
  • 7.3&nbsp; &nbsp; Fetal blood supply</p><p>

Chapter THREE

RESEARCH METHODOLOGY

  • </p><p>
  • 3.0&nbsp; &nbsp; Materials and Methods</p><p>
  • 3.1&nbsp; &nbsp; Materials</p><p>3.
  • 1.1Chemicals and reagents</p><p>
  • 3.2&nbsp; &nbsp; Methods</p><p>3.
  • 2.1Animal managements</p><p>3.
  • 2.2Feed composition</p><p>3.
  • 2.3&nbsp; &nbsp; Body weight determination</p><p>3.
  • 2.4&nbsp; &nbsp; Animal sacrifice</p><p>3.
  • 2.5&nbsp; &nbsp; Determination of parameters</p><p>
  • 3.3&nbsp; &nbsp; &nbsp; Determination of synaptosomal Na+-K+ ATPase activity</p><p>
  • 3.4&nbsp; &nbsp; Protein determination</p><p>

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • </p><p>
  • 4.0&nbsp; &nbsp; Results</p><p>

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • </p><p>
  • 5.0&nbsp; &nbsp; Discussion, Conclusion and Recommendation</p><p>
  • 5.1&nbsp; &nbsp; Discussion</p><p>
  • 5.2&nbsp; &nbsp; Conclusion</p><p>
  • 5.3&nbsp; &nbsp; Recommendation</p><p>References</p><p>Appendix</p><p>LIST OF FIGURES</p><p>Figure 1 – The growth curve for normal and protein undernourished rat fed with diet containing coconut oil</p><p>Figure 2 – The activities of sodium potassium ATPase in normal and protein undernourished rat</p><p>&nbsp;LIST OF TABLES</p><p>Table 1 – Animal group</p><p>Table 3- Showing organ weight to body weight ratio and feed</p><p>Consumption</p><p>&nbsp; ABBREVIATION</p><p>ADP/ATP &nbsp; Adenosine 5-diphosphate/ Adenosine 5 triphosphate</p><p>Na+ &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Sodium</p><p>BSA &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Bovine serum albumin</p><p>CuSO4 &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;Copper sulphate</p><p>EPA &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Environmental protection agency</p><p>ETC &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Electron transport chain</p><p>HCN &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Hydrogen cyanide</p><p>K+ &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Potassium</p><p>LP &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Low protein</p><p>Na2CO3 &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Sodium carbonate</p><p>NaOH &nbsp; &nbsp; &nbsp; &nbsp; Sodium hydroxide</p><p>NP &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Normal protein</p><p>PU &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Protein undernutrition</p><p></p></div><h3></h3><br> <br><p></p>

Project Abstract

<p> </p><p>The attempt of this study is to investigate the activities of sodium-potassium ATPase in the liver of normal and protein undernourished male weaning rats fed with diets containing coconut oil. Twenty weaning male rats were grouped into four groups, five rats for each. Group A were fed with normal diets, Group B were fed with low protein diets containing 5% casein, Group C were fed with normal diets and coconut oil and Group D were fed with low protein diet and coconut oil. After feeding for a period of six weeks, the rats were sacrificed using cervical dislocation. The liver was harvested and homogenized in iced cold buffer and centrifuged at 4000rpm for 15 minutes. The pellets were discarded and the supernatant was stored.</p><p>The result of this study shows that activities of activities of sodium-potassium ATPase in the rat liver were significantly reduced when fed with normal with coconut oil diets compared to normal diets only. However, it was observed that the activities of sodium-potassium ATPase in the rat liver were significantly increased when fed with low protein diets compared to normal diets only. The activities of sodium-potassium ATPase in the rat liver when fed with low protein and coconut oil remain constant against the normal diet, as there was no reaction occurring after a successful evaluation of the results. This result indicates that coconut oil inhibited the activity of sodium-potassium ATPase in the rat liver</p> <br><p></p>

Project Overview

Blazingprojects Mobile App

πŸ“š Over 50,000 Project Materials
πŸ“± 100% Offline: No internet needed
πŸ“ Over 98 Departments
πŸ” Software coding and Machine construction
πŸŽ“ Postgraduate/Undergraduate Research works
πŸ“₯ Instant Whatsapp/Email Delivery

Blazingprojects App

Related Research

Biology education. 4 min read

Effect of Interactive Virtual Labs on Conceptual Understanding and Inquiry Skills in...

What This Project Is About A straightforward study that tests whether using interactive virtual labs helps high school biology students understand key concepts ...

BP
Blazingprojects
Read more →
Biology education. 4 min read

Impact of inquiry-based learning on conceptual understanding and long-term retention...

What This Project Is About A straightforward look at how asking students to explore biology concepts through guided questions and experiments (inquiry-based lea...

BP
Blazingprojects
Read more →
Biology education. 4 min read

Assessing the Effectiveness of Inquiry-Based Learning on Conceptual Understanding of...

What This Project Is About A straightforward study that tests whether learning by asking questions and exploring concepts (inquiry-based learning) helps high sc...

BP
Blazingprojects
Read more →
Biology education. 3 min read

Assessment of the effectiveness of inquiry-based learning on high school biology ach...

What This Project Is About A plain-language overview of the topic and what the project investigates. The Problem It Addresses What problem or gap this project ...

BP
Blazingprojects
Read more →
Biology education. 2 min read

Analysis of the effectiveness of inquiry-based learning on high school biology achie...

What This Project Is About This project looks at how teaching biology through inquiry-based learning (IBL) affects what high school students know and how they f...

BP
Blazingprojects
Read more →
Biology education. 4 min read

Evaluating the effectiveness of inquiry-based learning modules on cellular biology c...

What This Project Is About A plain-language overview of evaluating how inquiry-based learning modules impact students’ understanding of cellular biology conce...

BP
Blazingprojects
Read more →
Biology education. 3 min read

Impact of Inquiry-Based Learning on Conceptual Understanding and Scientific Thinking...

What This Project Is About A plain-language overview of how teaching through asking questions and exploring ideas (inquiry-based learning) can affect how studen...

BP
Blazingprojects
Read more →
Biology education. 4 min read

Assessing the Impact of Inquiry-Based Learning on High School Biology Students’ Co...

What This Project Is About A plain-language overview of how teaching through inquiry helps students explore biology concepts and develop scientific thinking ski...

BP
Blazingprojects
Read more →
Biology education. 4 min read

Impact of Inquiry-Based Learning on High School Biology Exam Performance and Scienti...

What This Project Is About A straightforward examination of how using inquiry-based learning (IBL) affects students’ performance in high school biology exams ...

BP
Blazingprojects
Read more →
WhatsApp Click here to chat with us