THE OXIDATIVE STRESS STATUS OF RATS FED ON OIL BEAN SEED MEAL

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Oxidative Stress
  • 2.2Oil Bean Seed Meal as Animal Feed
  • 2.3Effects of Oil Bean Seed Meal on Rat Health
  • 2.4Antioxidants and Their Role in Oxidative Stress
  • 2.5Previous Studies on Oxidative Stress in Animal Models
  • 2.6Impact of Diet on Oxidative Stress
  • 2.7Role of Nutritional Supplements in Reducing Oxidative Stress
  • 2.8Methods to Measure Oxidative Stress Levels
  • 2.9Role of Lipids in Oxidative Stress
  • 2.10Impact of Oxidative Stress on Cellular Function

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Design
  • 3.2Sampling Techniques
  • 3.3Data Collection Methods
  • 3.4Data Analysis Procedures
  • 3.5Ethical Considerations
  • 3.6Instrumentation Used
  • 3.7Variables Considered
  • 3.8Research Framework

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Overview of Research Findings
  • 4.2Analysis of Oxidative Stress Levels in Rats
  • 4.3Comparison of Control and Experimental Groups
  • 4.4Impact of Oil Bean Seed Meal on Antioxidant Levels
  • 4.5Effects of Duration of Feeding on Oxidative Stress
  • 4.6Correlation between Diet and Oxidative Stress Biomarkers
  • 4.7Discussion on Lipid Peroxidation Levels
  • 4.8Interpretation of Cellular Damage from Oxidative Stress

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of Findings
  • 5.2Conclusion
  • 5.3Implications of the Study
  • 5.4Recommendations for Future Research
  • 5.5Contribution to the Field

Project Abstract

<p>This study was on oxidative stress status of rat fed with Pentraclethra macrophylla, otherwise known as African oil bean seed oil in English or Ugba in Igbo. Sixteen male rats were distributed into four groups. 1 (control), then group 2, 3, and 4 as test groups. They were fed with their formulated meal (5%, 10%, 20% inclussions) for 28 days. Group 1 was the control and were fed with the normal feed, while group 2, 3 and 4 which were the test groups were fed with test feed formula.. The parameters determined were MDA concentrations and catalase activity. Serum MDA significantly increased (p&lt;0.05) while the catalase activity significantly decreased (p&lt;0.05). This finding may be clinically significant to individuals with predisposition to increased generation of reactive oxygen species (ROS) and other degenerative diseases.<br><br><br></p>

Project Overview

<p><br>1.0. INTRODUCTION<br>Ugba also called ukpaka is a popular food delicacy in Nigeria especially among Igbo ethnic group. It is rich in protein and is obtained by a solid state fermentation of the seed of African oil bean tree (Pentaclethra macrophylla Benth).<br>The natural fermentation of the seed which at present is still done at the house-hold level, renders the production nutritious, palatable and non-toxic (Enujiugha, 2002).<br>Its production, like many African fermented foods depends, entirely on mixed fermentation by microorganism from diverse source.<br>Pentaclethra macrophylla Benth is a large woody plant abundant in the rain forest areas of west and central Africa. It’s origin in Nigeria is believed to be around 1937 (Ladipo, 1984); where it is found in the South Nigeria, (Mbajunwa et al., 1998).<br>―Ugba‖ Pentaclethra macrophylla Benth belongs to the Family Leguminosae and sub-family Microsoideae (Keay, 1989 and NFTA, 1995).<br>Ugba seeds are irregular and oval; they are flat, black and hard pods. It is composed of oil, protein and small amounts of carbohydrate (Obeta, 1982<br></p>

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