The effect of sodium citrate and reduced glutathione (gsh) on arginase from human fibriod tissue

 

Table Of Contents


  • <p> </p><p><strong><em>Abstract &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</em></strong></p><p><strong>

Chapter ONE

INTRODUCTION

  • </strong></p><p>
  • 1.0&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Introduction and Literature Review</p><p>
  • 1.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Fibroid</p><p>1.
  • 1.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Location and classification of fibroid</p><p>1.
  • 1.2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Pathogenesis and Pathophysiology</p><p>1.1.
  • 2.1&nbsp; &nbsp; &nbsp; Pathogenesis</p><p>1.1.
  • 2.2&nbsp; &nbsp; &nbsp; Pathophysiology</p><p>1.
  • 1.3&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Signs, Symptoms and Causes of fibroid</p><p>
  • 1.2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Arginase</p><p>1.
  • 2.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Role of arginase in human body</p><p>1.
  • 2.2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Aginase activities in Benign tumour</p><p>1.
  • 2.3&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Arginase II expression in benign and malignant prostate tissue</p><p>
  • 1.3&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Glutathione (GSH)</p><p>1.
  • 3.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Role of glutathione as an antioxidant</p><p>1.
  • 3.2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Glutathione response to exercise</p><p>
  • 1.4&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Sodium citrate</p><p>
  • 1.5&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Aims and objectives</p><p>

Chapter TWO

LITERATURE REVIEW

  • </p><p>
  • 2.0&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Materials and Methods</p><p>
  • 2.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Materials</p><p>2.
  • 1.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Apparatus</p><p>
  • 2.2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Methods</p><p>2.
  • 2.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Preparation of buffers and reagents</p><p>
  • 2.3&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Biochemical test</p><p>2.
  • 3.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Arginase assay</p><p>2.
  • 3.2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Protein standard curve</p><p>2.
  • 3.3&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Effect of Glutathione (GSH)</p><p>

Chapter THREE

RESEARCH METHODOLOGY

  • </p><p>
  • 3.0&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Results</p><p>

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • </p><p>
  • 4.0&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Discussion, Conclusion and Recommendation</p><p>
  • 4.1&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Discussion</p><p>
  • 4.2&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Conclusion</p><p>
  • 4.3&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Recommendations</p><p>References</p><p>LIST OF FIGURES</p><p>Figure 1 – &nbsp; &nbsp; Uterine Fibroid</p><p>Figure 2 – &nbsp; Hydrolysis of L-arginine</p><p>Figure 3 – &nbsp; Structure of arginase and the active site</p><p>Figure 4 – &nbsp; Showing the effect of sodium citrate on arginase from human fibroid tissue</p><p>Figure 5 – &nbsp; Showing the effect of Glutathione (GSH) on arginase from human fibroid tissue.</p> <br><p></p>

Project Abstract

<p> </p><p>The research is aimed at investigating the effect of sodium citrate and reduced glutathione (GSH) on arginase from human fibriod tissue.</p><p>The research shows that arginase enzyme which was partially purified was found to have a specific interaction with its substrate.</p><p>The study shows that varying the substrate concentration at constant volume of Sodium citrate and Reduced glutathione (GSH) resulted into an Un-competitive inhibition and Competitive inhibition of enzymes respectively.</p><p>The results obtained from this study shows that the inhibition nature of arginase by different inhibitors of enzymes gives a promising approach to abnormal arginase activities in cell proliferation of uterine muscle which leads to fibroid.</p> <br><p></p>

Project Overview

Blazingprojects Mobile App

📚 Over 50,000 Project Materials
📱 100% Offline: No internet needed
📝 Over 98 Departments
🔍 Software coding and Machine construction
🎓 Postgraduate/Undergraduate Research works
📥 Instant Whatsapp/Email Delivery

Blazingprojects App

Related Research

Biochemistry. 2 min read

Development of Enzymatic Biosensors for Rapid Detection of Environmental Pollutants...

What This Project Is About This project focuses on creating tiny devices called biosensors that can quickly detect harmful pollutants in the environment, such a...

BP
Blazingprojects
Read more →
Biochemistry. 3 min read

Optimization of Enzymatic Synthesis Pathways for Sustainable Biopolymer Production...

What This Project Is About This project explores ways to make the process of creating biopolymers more efficient and eco-friendly. Biopolymers are natural plast...

BP
Blazingprojects
Read more →
Biochemistry. 4 min read

Design and Characterization of Enzyme-Activated Nanocarriers for Targeted Cancer The...

What This Project Is About This project focuses on creating tiny particles called nanocarriers that can deliver medicine directly to cancer cells. These nanocar...

BP
Blazingprojects
Read more →
Biochemistry. 4 min read

Development of Novel Enzymatic Biosensors for Rapid Detection of Plant Pathogens...

This project is about developing a new type of tool called an enzymatic biosensor, which can quickly detect harmful bacteria or viruses that cause plant disease...

BP
Blazingprojects
Read more →
Biochemistry. 3 min read

Design and optimization of enzyme nanocarriers for targeted drug delivery in cancer ...

This project is about creating tiny carriers, called nanocarriers, that can deliver medicines directly to cancer cells. Scientists are interested in using enzym...

BP
Blazingprojects
Read more →
Biochemistry. 3 min read

Exploring the Role of Epigenetic Modifications in Cancer Development and Progression...

The project titled &quot;Exploring the Role of Epigenetic Modifications in Cancer Development and Progression&quot; aims to investigate the intricate relationsh...

BP
Blazingprojects
Read more →
Biochemistry. 3 min read

Exploring the Role of MicroRNAs in Cancer Progression and Therapeutic Resistance...

The project titled &quot;Exploring the Role of MicroRNAs in Cancer Progression and Therapeutic Resistance&quot; aims to investigate the intricate involvement of...

BP
Blazingprojects
Read more →
Biochemistry. 3 min read

Exploring the Role of MicroRNAs in Cancer Development and Progression...

The project topic, &quot;Exploring the Role of MicroRNAs in Cancer Development and Progression,&quot; focuses on investigating the intricate involvement of micr...

BP
Blazingprojects
Read more →
Biochemistry. 2 min read

Exploring the Role of MicroRNAs in Cancer Progression and Therapeutic Potential...

The project topic, &quot;Exploring the Role of MicroRNAs in Cancer Progression and Therapeutic Potential,&quot; delves into the intricate world of microRNAs and...

BP
Blazingprojects
Read more →
WhatsApp Click here to chat with us