Prevalence of salmonella spp. and e. coli on some automated teller machine (atm) surfaces within kaduna-north local government, kaduna state
Table Of Contents
- <p> </p><div><p>Title page – – – – – – – – – i</p><p>Dedication – – – – – – – – ii</p><p>Declaration – – – – – – – – – iii</p><p>Project Approval – – – – – – – – iv</p><p>Acknowledgement – – – – – – – – v</p><p>Table of content – – – – – – – – vi</p><p>Abstract – – – – – – – – – vi<strong>i</strong></p><p><strong>
Chapter ONE
INTRODUCTION
- </strong></p><p>
- 1.0 Introduction – – – – – – – – 1</p><p>
- 1.1 Aim – – – – – – – – – 4</p><p>
- 1.2 Objectives – – – – – – – – 4</p><p>
- 1.3Statement of Research Problem – – – – – – 4</p><p>
- 1.4 Justification – – – – – – – – 5</p><p><strong>
Chapter TWO
LITERATURE REVIEW
- </strong></p><p>
- 2.0 Literature review- – – – – – – – 6</p><p>
- 2.1 An automated Teller Machine (ATM machine) – – – – 6</p><p>
- 2.2 History and invention of ATM machines – – – – 6</p><p>
- 2.3 ATMs contact surfaces – – – – – – – 7</p><p>
- 2.4 Hygiene – – – – – – – – – 8</p><p>
- 2.5Poor personal hygiene – – – – – – – 8</p><p>
- 2.6 Cross contamination – – – – – – – 9</p><p>
- 2.7 Outbreak associated with ATM contact surfaces- – – – 9</p><p>
- 2.8 Contributing factors to illness- – – – – – – 10</p><p>
- 2.9Enteric Bacteria – – – – – – – – 13</p><p>2.
- 1.1Selected genera of the Family <em>Enterobacteriaceae</em> – – – 16</p><p>2.1.2<em>Escherichia coli</em>– – – – – – – – – 16 </p><p>2.
- 1.3<em>Salmonella species.</em> – – – – – – – 21</p><p><strong>
Chapter THREE
RESEARCH METHODOLOGY
- </strong></p><p>
- 3.0Materials and methods – – – – – – – 23</p><p>
- 3.1Sampling sites – – – – – – – – 23</p><p>
- 3.2 Sample collection – – – – – – – 23</p><p>
- 3.3 Media preparation – – – – – – – 24</p><p>
- 3.4 Bacteriological Procedures – – – – – – 24</p><p>3.
- 4.1 Serial dilution – – – – – – – – 24</p><p>3.
- 4.2 Inoculation and incubation of samples – – – – 25</p><p>3.
- 4.3Gram staining – – – – – – – – 26</p><p>
- 3.5 Biochemical tests – – – – – – – 27</p><p>3.
- 5.1 Citrate utilization test – – – – – – – 27</p><p>3.
- 5.2 Indole test – – – – – – – – 27</p><p>3.
- 5.3 Methyl red test – – – – – – – – 27</p><p>3.
- 5.4 Voges-Proskauer test – – – – – – – 28</p><p>3.
- 5.5 Triple sugar iron test – – – – – – – 28</p><p>3.
- 5.6 Urease test – – – – – – – – 28</p><p><strong>
Chapter FOUR
DATA PRESENTATION AND ANALYSIS
- </strong></p><p>
- 4.0 Results – – – – – – – – – 30</p></div><h3></h3><br> <br><p></p>
Project Abstract
Automated Teller Machines (ATMs) are vital touchpoints in the daily lives of individuals for financial transactions. The prevalence of pathogenic microorganisms on ATM surfaces poses a potential public health risk. This study aimed to investigate the prevalence of Salmonella spp. and E. coli on selected ATM surfaces within Kaduna-North Local Government, Kaduna State. A total of 50 ATM surfaces were sampled using sterile swabs and then cultured on selective media for the isolation of Salmonella spp. and E. coli. The isolates were identified using standard biochemical tests and confirmed through molecular techniques. The prevalence of Salmonella spp. and E. coli was determined as the percentage of positive samples out of the total samples collected. The results revealed that 18 out of the 50 sampled ATM surfaces were positive for Salmonella spp., indicating a prevalence rate of 36%. Similarly, E. coli was isolated from 25 out of the 50 samples, giving a prevalence rate of 50%. Molecular confirmation further validated the presence of these pathogens on the ATM surfaces. The findings of this study highlight a concerning prevalence of Salmonella spp. and E. coli on ATM surfaces within Kaduna-North Local Government, Kaduna State. These results underscore the importance of regular cleaning and disinfection of ATM surfaces to mitigate the risk of pathogen transmission to users. Public health awareness campaigns should also be implemented to educate individuals on the potential risks associated with touching contaminated ATM surfaces. In conclusion, the study showcases the need for enhanced hygiene practices and monitoring of ATM surfaces to ensure public health and safety. Further research could explore the antimicrobial resistance profiles of the isolated pathogens and assess the effectiveness of different disinfection methods in reducing pathogen contamination on ATM surfaces. Implementing stringent hygiene protocols and routine maintenance of ATMs can help minimize the transmission of pathogenic microorganisms and safeguard the health of ATM users and the general public.
Project Overview