Prevalence of salmonella spp. and e. coli on some automated teller machine (atm) surfaces within kaduna-north local government, kaduna state
Table Of Contents
- <p> </p><p>Title page – – – – – – – – – i</p><p>Dedication – – – – – – – – ii</p><p>Declaration – – – – – – – – – iii</p><p>Project Approval – – – – – – – – iv</p><p>Acknowledgement – – – – – – – – v</p><p>Table of content – – – – – – – – vi</p><p>Abstract – – – – – – – – – vi<strong>i</strong></p><p><strong>
Chapter ONE
INTRODUCTION
- </strong></p><p>
- 1.0 Introduction – – – – – – – – 1</p><p>
- 1.1 Aim – – – – – – – – – 4</p><p>
- 1.2 Objectives – – – – – – – – 4</p><p>
- 1.3Statement of Research Problem – – – – – – 4</p><p>
- 1.4 Justification – – – – – – – – 5</p><p><strong>
Chapter TWO
LITERATURE REVIEW
- </strong></p><p>
- 2.0 Literature review- – – – – – – – 6</p><p>
- 2.1 An automated Teller Machine (ATM machine) – – – – 6</p><p>
- 2.2 History and invention of ATM machines – – – – 6</p><p>
- 2.3 ATMs contact surfaces – – – – – – – 7</p><p>
- 2.4 Hygiene – – – – – – – – – 8</p><p>
- 2.5Poor personal hygiene – – – – – – – 8</p><p>
- 2.6 Cross contamination – – – – – – – 9</p><p>
- 2.7 Outbreak associated with ATM contact surfaces- – – – 9</p><p>
- 2.8 Contributing factors to illness- – – – – – – 10</p><p>
- 2.9Enteric Bacteria – – – – – – – – 13</p><p>2.
- 1.1Selected genera of the Family <em>Enterobacteriaceae</em> – – – 16</p><p>2.1.2<em>Escherichia coli</em>– – – – – – – – – 16 </p><p>2.
- 1.3<em>Salmonella species.</em> – – – – – – – 21</p><p><strong>
Chapter THREE
RESEARCH METHODOLOGY
- </strong></p><p>
- 3.0Materials and methods – – – – – – – 23</p><p>
- 3.1Sampling sites – – – – – – – – 23</p><p>
- 3.2 Sample collection – – – – – – – 23</p><p>
- 3.3 Media preparation – – – – – – – 24</p><p>
- 3.4 Bacteriological Procedures – – – – – – 24</p><p>3.
- 4.1 Serial dilution – – – – – – – – 24</p><p>3.
- 4.2 Inoculation and incubation of samples – – – – 25</p><p>3.
- 4.3Gram staining – – – – – – – – 26</p><p>
- 3.5 Biochemical tests – – – – – – – 27</p><p>3.
- 5.1 Citrate utilization test – – – – – – – 27</p><p>3.
- 5.2 Indole test – – – – – – – – 27</p><p>3.
- 5.3 Methyl red test – – – – – – – – 27</p><p>3.
- 5.4 Voges-Proskauer test – – – – – – – 28</p><p>3.
- 5.5 Triple sugar iron test – – – – – – – 28</p><p>3.
- 5.6 Urease test – – – – – – – – 28</p><p><strong>
Chapter FOUR
DATA PRESENTATION AND ANALYSIS
- </strong></p><p>
- 4.0 Results – – – – – – – – – 30</p> <br><p></p>
Project Abstract
Automated Teller Machines (ATMs) are widely used by the public for financial transactions, and their surfaces can serve as potential reservoirs for various pathogens. This study aimed to investigate the prevalence of Salmonella spp. and E. coli on ATM surfaces within Kaduna-North Local Government, Kaduna State. A total of 50 ATM surfaces were swabbed and analyzed for the presence of these bacteria using standard microbiological techniques. The results revealed that 32% of the sampled ATM surfaces were contaminated with Salmonella spp., while 18% were contaminated with E. coli. The most frequently contaminated areas were the keypad and the card insertion slot. The presence of these pathogenic bacteria on ATM surfaces poses a potential public health risk as they can be easily transferred to individuals during ATM usage. Furthermore, antibiotic susceptibility testing was conducted on the isolated Salmonella spp. and E. coli strains. The results indicated varying levels of resistance to commonly used antibiotics, highlighting the importance of regular monitoring and proper hygiene practices in public places like ATMs to prevent the spread of antimicrobial-resistant bacteria. Overall, this study underscores the need for increased awareness about the potential microbial contamination of ATM surfaces and the importance of routine cleaning and disinfection to mitigate the risk of bacterial transmission. Implementing strict hygiene protocols and regular monitoring of ATM surfaces for bacterial pathogens can help reduce the spread of infectious diseases in the community. Future research should focus on exploring additional strategies to maintain the cleanliness of public touchpoints like ATMs and enhance public health safety measures in high-traffic areas.
Project Overview