Life cycle assessment of the environmental impact of biosurfactant production from oil waste by a diculture of azotobacter vinelandii and pseudomonas sp.
Table Of Contents
Chapter ONE
INTRODUCTION
- 1.1Introduction
- 1.2Background of Study
- 1.3Problem Statement
- 1.4Objective of Study
- 1.5Limitation of Study
- 1.6Scope of Study
- 1.7Significance of Study
- 1.8Structure of the Research
- 1.9Definition of Terms
Chapter TWO
LITERATURE REVIEW
- 2.1Overview of Biosurfactants
- 2.2Environmental Impact of Biosurfactant Production
- 2.3Azotobacter Vinelandii: Characteristics and Applications
- 2.4Pseudomonas sp.: Characteristics and Applications
- 2.5Microbial Diculture in Biosurfactant Production
- 2.6Oil Waste as a Substrate for Biosurfactant Production
- 2.7Methods of Biosurfactant Production
- 2.8Market Trends and Applications of Biosurfactants
- 2.9Sustainable Production Practices
- 2.10Global Initiatives for Environmental Sustainability
Chapter THREE
RESEARCH METHODOLOGY
- 3.1Research Design and Methodology
- 3.2Selection of Microbial Strains
- 3.3Cultivation and Growth Conditions
- 3.4Biosurfactant Extraction Methods
- 3.5Analytical Techniques for Characterization
- 3.6Environmental Impact Assessment Methods
- 3.7Data Collection and Analysis
- 3.8Statistical Tools and Software Used
Chapter FOUR
DATA PRESENTATION AND ANALYSIS
- 4.1Analysis of Biosurfactant Production Efficiency
- 4.2Characterization of Biosurfactants Produced
- 4.3Environmental Impact Assessment Results
- 4.4Comparison with Conventional Surfactants
- 4.5Economic Analysis of Production Process
- 4.6Discussion on Sustainability and Scalability
- 4.7Recommendations for Process Optimization
- 4.8Future Research Directions
Chapter FIVE
SUMMARY, CONCLUSION AND RECOMMENDATIONS
- 5.1Summary of Findings
- 5.2Conclusions Drawn from the Study
- 5.3Implications for Environmental Sustainability
- 5.4Recommendations for Industry and Policy
- 5.5Reflection on Research Process
- 5.6Contribution to Scientific Knowledge
- 5.7Limitations and Areas for Future Research
- 5.8Closing Remarks and Acknowledgments
Project Abstract
<p> During the bioprocess of the biosurfactant production (by the consortium), the volume of CO2 evolved was 28.23 ± 5.08 cm3, which is equivalent to 0.056 ± 0.01g CO2 per 100 ml of broth. In terms of gCO2/1000Kg biosurfactant, this gave a value of 4545 ± 817.93g CO2 in this bioprocess. Following from above, the life cycle impact assessment (LCIA) of biosurfactant production by this consortium, based on global warming potential (GWP) was 0.046 tonnes/1000Kg biosurfactant. Other impact values calculated for acidification and eutrophication potentials were 0.008 tonnes / 1000 Kg and 0.0014 tonnes/1000Kg of biosurfactant. These values were considered insufficient in terms of environmental pollution when compared with the regular methods of surfactants production. In this work also, the consortium of Pseudomonas sp/Azotobacter vinelandii produced 1.22 ± 0.04 mg biosurfactant per 100ml of cell – free broth. However, the individual organisms (Pseudomonas sp. and Azotobacter vinelandii) produced 1.03 ± 0.02 and 0.08 ± 0.001 mg of biosurfactants per 100 ml cell – free broth respectively. The microbial growth kinetics during the production of the biosurfactant by the consortium gave a specific maximum growth (µmax) of 1.306 ± 0.201 hr-1), a saturation constant (Ks) of 0.017 ± 0.007 mg/l, an Inhibition constant (Ki) of 121.83 ± 21.18 mg/land a Death constant (Kd) of 0.017 ± 0.006 mg/l. These values when compared with those of individual organisms shows that using a consortium for the bioprocess is more sustainable. <br></p>Project Overview
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