Isolation and characterization of antibiotic producing actinomycetes in a rhizosphere environment

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Actinomycetes
  • 2.2History of Antibiotic Production
  • 2.3Role of Actinomycetes in Rhizosphere Environment
  • 2.4Antibiotic Resistance Mechanisms
  • 2.5Methods of Isolation and Characterization
  • 2.6Factors Influencing Antibiotic Production
  • 2.7Importance of Studying Antibiotic Producing Actinomycetes
  • 2.8Relationship Between Actinomycetes and Plant Health
  • 2.9Commercial Applications of Actinomycetes
  • 2.10Current Trends in Actinomycetes Research

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Design
  • 3.2Sampling Techniques
  • 3.3Isolation Methods
  • 3.4Characterization Techniques
  • 3.5Data Collection Procedures
  • 3.6Data Analysis Methods
  • 3.7Ethical Considerations
  • 3.8Research Limitations

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Overview of Research Findings
  • 4.2Distribution of Antibiotic Producing Actinomycetes
  • 4.3Characterization of Isolated Strains
  • 4.4Antibiotic Production Profiles
  • 4.5Influence of Environmental Factors
  • 4.6Comparison with Known Antibiotics
  • 4.7Potential Applications of Isolated Actinomycetes
  • 4.8Implications for Future Research

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Conclusion and Summary
  • 5.2Key Findings Recap
  • 5.3Contributions to the Field
  • 5.4Recommendations for Future Studies
  • 5.5Final Remarks

Project Abstract

<p> Two hundred and forty isolates were isolated from the cow dung, compost, soils where the hides and skin of animals were burnt. Three isolates designated A1, A7, and A43 displayed clear zones of inhibition characteristic of Actinomycetes. The isolates were further characterized by examining their colony morphology, cover slip culture techniques, carbon utilization potentials, gram staining reaction and amylase activity. The α-amylase activity was determined on solid medium supplemented with starch. The detection α-amylase activity of the isolates was based on the formation of clear zones around the colonies when flooded with iodine. The decolourization abilities of the isolates were also determined. The isolates showed good absorption characteristics and degrading capacity on the dye Congo red and brilliant green. It showed that Actinomycetes are potential decolorizer of dye stuffs and are good biosorbent. <br></p>

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