INVESTIGATION OF THE PANCREATIC EFFECT OF BUCCHOLZIA CORICACEA FORMULATED DIET IN SUCROSE FED PREGNANT RATS AND THEIR OFFSPRING

Project Abstract

The aim of this study was to investigate the pancreatic effect of Buchholzia coriacea formulated diet in sucrose-fed pregnant rats and their offspring. Sucrose consumption during pregnancy has been linked to adverse effects on pancreatic function in both mothers and offspring. Buchholzia coriacea, a plant with known anti-diabetic properties, was hypothesized to mitigate the negative impact of sucrose on pancreatic health. Thirty female Wistar rats were randomly divided into five groups Control, Sucrose, Buchholzia coriacea, Sucrose+Buchholzia coriacea, and Buchholzia coriacea only. The rats were maintained on their respective diets before and during pregnancy. After delivery, the offspring had access to the same diets as their mothers until weaning. Fasting blood glucose levels, serum insulin levels, pancreatic histology, and pancreatic oxidative stress markers were assessed. The results showed that sucrose-fed pregnant rats had significantly higher fasting blood glucose levels, lower serum insulin levels, and increased pancreatic oxidative stress compared to the control group. However, rats supplemented with Buchholzia coriacea showed improved pancreatic health, with reduced oxidative stress levels and improved pancreatic histology. Offspring from mothers on the Buchholzia coriacea diet also exhibited better pancreatic function compared to the sucrose group. These findings suggest that Buchholzia coriacea supplementation during pregnancy has a protective effect on the pancreas in sucrose-fed rats and their offspring. Further research is needed to elucidate the mechanisms underlying these effects and to determine the potential implications for human health.

Project Overview

<p> </p><ul><li><strong>NTRODUCTION</strong></li><li><strong>BACKGROUND OF THE STUDY</strong></li></ul><p>Herbal medicine is the oldest form of health care known to mankind. The use of medicinal plant in the treatment of diseases has been in practice since ancient time in different parts of the world especially in Africa. Plants have always been the most vital source of drugs mainly because most plants are autotrophs, and are able to synthesize a large variety of basic biochemical and organic substances such as carbohydrates, protein, terpenes, steroids, alkaloid and glycosides (N’guessan<em>etal.,</em>2009)</p><p>The plant kingdom provides a tremendous reservoir of various chemical substances with potential therapeutic properties. Generally, plants which produce constituents having medical values are designated as medicinal plants (Lawrence <em>et al.,</em>2008). In addition, all plants that taste bitter are used as medicine (Barrett, 2009).</p><p>Diabetes mellitus is characterize by insufficient blood levels of the hormone insulin. If the blood concentration of insulin is too low, muscle and liver cells do not absorb glucose from the blood which in turn leads to increase levels of blood glucose (hyperglycemia), impaired metabolism of fats and proteins, ketosis and possible diabetic coma. (willam,<em>&nbsp;et.al</em>&nbsp;2009)During the past 12 years, the world health organization has been collecting information on the prevalence of diabetes mellitus in adult communities worldwide. Within the age range of 30-64 years, diabetes was found to be absent or rare in some traditional communities in Melanesia, East Africa and South America. In communities of Europeanorigin, the prevalence of diabetes were in the range of 3-10% but migrant indian, Chinese and Hispanic American groups were at higher risk (15-20%). (Adetokunbo, <em>et.al.,</em>2003)</p><p>A 2008 study completed in U.S. found the number of America women entering pregnancy with pre-exisiting diabetes is increasing. In fact, the rate of diabetes in expectant mothers has more than doubled in the past six years. (Lawrence,<em>et.al.,</em>2008). This is particularly problematic as diabetes raises the risk of complications during pregnancy, as well as increasing the potential for the children of diabetic mothers to become diabetic in the future</p><ul><li><strong>STATEMENT OF THE PROBLEM</strong></li></ul><p>Malnutrition is define as the lack of sufficient nutrients, which are essential for the body’s normal functioning. Over time it affects the bodily organs and results in mild to severe medical problems. One of the malnutrition facts is that the number of hungry people is more in the developing countries. If a pregnant woman is malnourished, it is understandable that the baby in the mother’s womb is not receiving enough nutrient.</p><p>Pregnant women who have been through malnutrition, deliver babies with low birth weight. Such children are prone to retarded growth, less coordination, poor vision, learning difficulty, and many other diseases. Anemia is one of the malnutrition dises that affects several pregnant women worldwide.</p><ul><li><strong>AIM OF THE STUDY</strong></li></ul><p>To investigate the effect of high sucrose fed pregnant rats in their offsprings and know their pancreatic effect. And also the effect of <em>Buccholozia Coriacea</em></p><p><strong>1.4 OBJECTIVES OF THE STUDY</strong></p><p>Induce diabetes by administering sucrose</p><p>Administering plants for lowering blood sugar level</p><p>Measure the blood and leptin level</p> <br><p></p>