Estimation of total serum level of protein and iron in mice infected with malaria parasite (plasmodium berghei, berghei) and treated with tetracycline

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Total Serum Protein
  • 2.2Functions of Protein in the Body
  • 2.3Iron Metabolism in Mice
  • 2.4Relationship between Malaria Infection and Protein Levels
  • 2.5Tetracycline Treatment in Malaria Management
  • 2.6Effects of Malaria Parasite Infection on Iron Levels
  • 2.7Previous Studies on Protein and Iron in Mice
  • 2.8Impact of Tetracycline on Iron Absorption
  • 2.9Role of Protein in Immune Response to Malaria
  • 2.10Iron Deficiency Anemia in Malaria-Infected Mice

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Design and Rationale
  • 3.2Sampling Methodology
  • 3.3Data Collection Procedures
  • 3.4Data Analysis Techniques
  • 3.5Ethical Considerations in Animal Studies
  • 3.6Experimental Setup and Variables
  • 3.7Statistical Tools for Analysis
  • 3.8Validation of Results

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Total Serum Protein Levels in Infected Mice
  • 4.2Iron Concentrations in Malaria-Infected Mice
  • 4.3Impact of Tetracycline Treatment on Protein Levels
  • 4.4Changes in Iron Levels post-Tetracycline Treatment
  • 4.5Correlation between Protein and Iron Levels
  • 4.6Comparison with Control Group
  • 4.7Discussion on Immune Response and Protein Synthesis
  • 4.8Implications for Malaria Treatment

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of Findings
  • 5.2Conclusion and Interpretation
  • 5.3Recommendations for Future Research
  • 5.4Practical Applications of the Study
  • 5.5Contribution to Scientific Knowledge

Project Abstract

<p> The serum has been a center of infection of all sorts, in which most diseases associated with the body revolve, causing alterations in the body’s system. Malaria, as a disease condition of the body (especially in mice) results from inflections with the malaria parasite introduced into the blood stream. (malaria parasite introduced is plasmodium berghei, berghei). Parameters related to the blood of which investigation was concerned were those involving serum protein and iron. A total of 20 mice, 15 with malaria infection, and 5 are non-infected mice (mice) also treated with tetracycline were used for the analysis. The following results were obtained in the end of the study and they are as follows; five animals infected, treated with (10mg/kg, underdose) of tetracycline has the total serum proteins and iron of mean and standard deviation for infected as 3.79 ± 0.96 and 2125.72 ± 947.5 respectively but the mean and standard deviation of control group were, (3.83 ± 1.33) and (1184.2 ± 473.6). At (20mg/kg normal dose = 3.95 ± 0.65) and 1547.14 and the control group were 3.83 ± 1.33 and 1184.2 ± 473.6. At (30mg/kg overdose) has the mean and standard deviation of total proteins and iron as 3.78 ± 0.55 and 1168.58 ± 360. 9 respectively. The reduction in iron level as the dose of the drugs increased is as a result of the chelating ability of tetracycline, significant changes also occurred for proteins. The biochemical implications of these results are these discussed. <br></p>

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