Effect of metal ions on arginase from the kidney of local goat (capra aegagrus hircus)

 

Table Of Contents


  • <p> </p><p>
  • 1.0&nbsp; &nbsp; Introduction and Literature review</p><p>
  • 1.1&nbsp; &nbsp; Introduction</p><p>
  • 1.2&nbsp; &nbsp; Literature review</p><p>1.
  • 2.1History of arginase</p><p>
  • 1.3&nbsp; &nbsp; Occurrence and distribution of arginase</p><p>1.
  • 3.1Bacteria</p><p>1.
  • 3.2Fungi</p><p>1.
  • 3.3Lichens</p><p>1.
  • 3.4Yeast</p><p>1.
  • 3.5Insects</p><p>
  • 1.4&nbsp; &nbsp; The substrate: Arginase</p><p>
  • 1.5&nbsp; &nbsp; Scientific classification of local goat</p><p>
  • 1.6&nbsp; &nbsp; Aim and Objectives</p><p>

Chapter TWO

LITERATURE REVIEW

  • </p><p>
  • 2.0&nbsp; &nbsp; Materials and methods</p><p>
  • 2.1&nbsp; &nbsp; Materials</p><p>2.
  • 1.1Apparatus used</p><p>
  • 2.2&nbsp; &nbsp; Methods</p><p>2.
  • 2.1Assay</p><p>2.
  • 3.2Protein determination</p><p>2.
  • 3.3Purification procedure</p><p>

Chapter THREE

RESEARCH METHODOLOGY

  • </p><p>
  • 3.0&nbsp; &nbsp; Results</p><p>
  • 3.1&nbsp; &nbsp; Purification and Homogeneity</p><p>
  • 3.2&nbsp; &nbsp; Effect of metal ion on arginase from kidney of a local goat</p><p>

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • </p><p>
  • 5.0&nbsp; &nbsp; Discussion, Conclusion and Recommendation</p><p>
  • 5.1&nbsp; &nbsp; Discussion</p><p>
  • 5.2&nbsp; &nbsp; Conclusion</p><p>
  • 5.3&nbsp; &nbsp; Recommendations</p><p>References.</p> <br><p></p>

Project Abstract

<p> This research is aimed at isolating, partially purifying and determination of arginase from the kidney of a local goat. Methods used in the isolation and partial purification of the enzyme include; Ammonium sulphate precipitation, dialysis.etc. Result from this work showed that arginase isolated from a local goat has a specific activity of 0.358 with a yield of 12.12% incubation of the enzyme with different metal ions shows that the enzyme is inhibited by Nacl and Bacl2. <br></p>

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