Effect of different carbon sources on the growth of antimicrobial producing bacillus species in citrillus vugaris.

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Carbon Sources
  • 2.2Growth Requirements of Bacillus Species
  • 2.3Role of Carbon Sources in Microbial Growth
  • 2.4Effect of Different Carbon Sources on Antimicrobial Production
  • 2.5Citrullus Vulgaris as a Growth Medium
  • 2.6Characteristics of Antimicrobial Producing Bacillus Species
  • 2.7Factors Influencing Microbial Growth in Citrullus Vulgaris
  • 2.8Importance of Studying Carbon Sources on Bacillus Growth
  • 2.9Previous Studies on Carbon Sources and Microbial Growth
  • 2.10Relationship Between Carbon Sources and Antimicrobial Activity

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Methodology Overview
  • 3.2Selection of Bacillus Species for Study
  • 3.3Preparation of Growth Media with Different Carbon Sources
  • 3.4Inoculation and Incubation Procedures
  • 3.5Measurement of Growth Parameters
  • 3.6Analysis of Antimicrobial Production
  • 3.7Statistical Methods for Data Analysis
  • 3.8Ethical Considerations in Research

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Analysis of Growth Patterns with Different Carbon Sources
  • 4.2Comparison of Antimicrobial Production Levels
  • 4.3Influence of Carbon Sources on Bacillus Species Morphology
  • 4.4Relationship Between Carbon Sources and Metabolic Pathways
  • 4.5Discussion on Optimal Carbon Sources for Bacillus Growth
  • 4.6Interpretation of Data on Antimicrobial Activity
  • 4.7Implications of Findings on Microbial Ecology
  • 4.8Comparison with Previous Research

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of Research Findings
  • 5.2Conclusions Drawn from the Study
  • 5.3Recommendations for Future Research
  • 5.4Practical Applications of the Study
  • 5.5Contribution to Scientific Knowledge

Project Abstract

<p> Effect of different carbon sources on the growth of antimicrobial producing Bacillus species in ogiri-egusi sold in Ogbeta, Orie, Artisian, Kenyatta, Enugu State were evaluated using standard microbiological and analytical methods. 12 bacteria were isolated and identified such as Bacillus subtilis,(33.33), Bacillus pumulis (41.66), Bacillus licheniformis (25) were identified based on their colony biochemical characteristics.The isolates of the highest occurrences is bacillus pumilus. Agar well diffusion method was used to determine the antimicrobial activity against two pathogenic organisms, Escherichia coli, Staphylococcus aureus. Bacillus subtilis and Bacillus pumulis, gave a zone of inhibition Escherichia coli 0.6mm and 0.8mm after 24 hours of incubation also carbon sources of 5% and 10% sucrose, Glucose and soluble starch were used to check their different carbon sources on the isolates using the spectrophotometer.5% had the highest optimum growth of 1.400 for glucose 1.545 for sucrose,2.376 for soluble starch which can be added in maximum quantity to a medium to produce antibiotics. <br></p>

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