Assessment of genetic (via protein electrophoresis) and nutritional variabilities among ten accessions of sphenostylis stenocarpa (hocht. ex. a. rich) harms

 

Table Of Contents


Chapter ONE

INTRODUCTION

  • 1.1Introduction
  • 1.2Background of Study
  • 1.3Problem Statement
  • 1.4Objective of Study
  • 1.5Limitation of Study
  • 1.6Scope of Study
  • 1.7Significance of Study
  • 1.8Structure of the Research
  • 1.9Definition of Terms

Chapter TWO

LITERATURE REVIEW

  • 2.1Overview of Genetic Variability
  • 2.2Protein Electrophoresis Techniques
  • 2.3Nutritional Variability in Plant Species
  • 2.4Importance of Studying Genetic Variability
  • 2.5Previous Studies on Sphenostylis Stenocarpa
  • 2.6Factors Influencing Genetic and Nutritional Variability
  • 2.7Methods for Assessing Genetic Variability
  • 2.8Protein Electrophoresis in Genetic Studies
  • 2.9Nutritional Analysis Techniques
  • 2.10Relationship Between Genetics and Nutrition

Chapter THREE

RESEARCH METHODOLOGY

  • 3.1Research Design and Methodology
  • 3.2Sampling Techniques
  • 3.3Data Collection Methods
  • 3.4Data Analysis Procedures
  • 3.5Experimental Setup
  • 3.6Statistical Tools for Analysis
  • 3.7Validation of Results
  • 3.8Ethical Considerations in Research

Chapter FOUR

DATA PRESENTATION AND ANALYSIS

  • 4.1Analysis of Genetic Variabilities
  • 4.2Assessment of Nutritional Variabilities
  • 4.3Interpretation of Findings
  • 4.4Comparison Among Accessions
  • 4.5Identification of Key Variabilities
  • 4.6Implications of Variabilities
  • 4.7Recommendations for Further Research
  • 4.8Future Applications of the Study

Chapter FIVE

SUMMARY, CONCLUSION AND RECOMMENDATIONS

  • 5.1Summary of Findings
  • 5.2Conclusion
  • 5.3Contributions to the Field
  • 5.4Research Implications
  • 5.5Practical Applications
  • 5.6Limitations of the Study
  • 5.7Recommendations for Future Studies
  • 5.8Closing Remarks

Project Abstract

<p> The genetic variability among ten (10) accessions of Sphenostylis stenocarpa from three states and National Centre for Genetic Resources and Biotechnology Moor Plantation, Ibadan, Nigeria were assessed using sodium dodecyl polyacrylamide gel electrophoresis (SDS – PAGE). There were variations in the banding patterns of the accessions and polymorphism was also observed. Twelve polypeptide bands were obtained ranging from 9KDA to 219KDa. The Unweighted Pair Group Method with Mean Algorithm (UPGMA) dendrogram grouped the ten accessions into two clusters and six groups. Some accessions appeared in the same group, while others were observed to be in different groups. The proximate and nutritional analysis also detected variations. A strong and positive correlation was observed among some of the attributes studied. The principal component analysis also showed some variation among the accessions. The highest variability was observed in the ash content which shows that ash content could be a major indicator for nutritional variability in these 10 accessions followed by the Vitamin B2 in the second component. <br></p>

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