Alterations in serum enzyme activities of rats on oral doses of mistletoe (globimetula cupulata (dc) van tieghem) leaf extract and organ histology

Project Abstract

<p> Alterations in serum enzyme activities of normal and alloxan-induced diabetic rats on administration of oral doses of ethanolic extract of <em>Globimetula cupulata</em>&nbsp;leaves were investigated in 6 groups of rats (5 rats per group). Test groups were made diabetic with intraperitoneal injection of alloxan and treated with 450mg and 900mg/kg body weight of the leaves extract. Two non-diabetic groups were also administered with 450mg and 900mg/kg body weight extract. The levels of serum enzyme activities were examined in all 6 experimental groups. Enzymes that were examined include Alkaline Phosphatase (ALP), Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT). In diabetic rats, enzyme activities were significantly reduced (p&lt;0.01) by 0.96-78.23% on consumption of the extracts, with greater effect exhibited by the 900mg/kg extract. In non-diabetic rats, enzyme activities were reduced by 0.19 – 17.95% on consumption of the leaves extract. Histological Studies shows that the extract had no adverse effect on the liver and heart although there may be a possible toxicity effect in the heart as the does increases as shown in the histology of the non-diabetic groups this results suggest that ethanolic extract of &nbsp;<em>G</em>. <em>cupulata </em>can be utilized in the management of various ailments such as diabetes mellitus, hypertension, among others without adverse effects on the liver and heart if a standard dose is set up and followed closely. <br></p>

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