EFFECT OF SOY GROUNDNUT MIXED GRAIN COMPLEMENTARY FOOD ON THE LEVEL OF SELECTED GROWTH IN MALE WEANING WISTAR RATS

Project Abstract

The transition from exclusive breastfeeding to complementary feeding is a critical period in the growth and development of infants. This study aimed to investigate the effect of soy-groundnut mixed grain complementary food on selected growth parameters in male weaning Wistar rats. Forty-eight male Wistar rats, weaned at 21 days, were randomly divided into four groups Group 1 (control) received standard rat chow, while Groups 2, 3, and 4 were fed soy-groundnut mixed grain complementary food at varying concentrations as 10%, 20%, and 30%, respectively, for a period of four weeks. The rats were monitored for changes in body weight, length, and selected biochemical parameters including serum albumin, total protein, and serum iron levels. Results showed a significant increase in body weight gain in the rats fed with soy-groundnut mixed grain complementary food compared to the control group. The group fed with 30% soy-groundnut mixed grain complementary food demonstrated the highest body weight gain among the treatment groups. Additionally, the length measurements showed a similar trend with a significant increase in body length in the groups receiving the mixed grain complementary food compared to the control group. The biochemical analysis revealed higher serum albumin, total protein, and serum iron levels in the rats fed with soy-groundnut mixed grain complementary food, with the highest levels detected in the group fed with 30% concentration. These findings suggest that the incorporation of soy-groundnut mixed grain complementary food in the diet of weaning rats positively influences growth parameters and selected biochemical markers. The protein-rich nature of the soy and groundnut may have contributed to the enhanced growth observed in the treatment groups. The results highlight the potential of utilizing soy-groundnut mixed grain complementary food as a nutritious option for weaning diets to support growth and development in male Wistar rats. Further studies are warranted to explore the long-term effects and potential mechanisms underlying the observed growth-promoting effects of this complementary food blend.

Project Overview

1.0      INTRODUCTION1.1   BACKGROUND OF STUDYSoybean is one of the most economical and nutritious foods which can combat the diseases stemming from malnutrition and the nutraceutical ingredients present in it and can reduce the risk of major chronic diseases like cancer, atherosclerosis, osteoporosis and diabetes (Vineet, 2007). Soybean is an excellent source of quality protein and compares well with animal protein in essential amino acids pattern with the exception of sulphur containing amino acids. Soybean is the concentrated source of vegetable proteins. It contains about 40%protein and 20% oil.Proteins are abundantly rich in lysine which is a limiting amino acid in most of the cereals. Soy fat being highly unsaturated has been found to be nutritionally desirable. Therefore, it can easily supplement the diet of poor Indians (Chauhan, 2005). It has high proportion of unsaturated fatty acids, such as linoleic and linolenic acids, it is considered as highly healthful oil. The ratio of polyunsaturated fatty acid to saturated fatty acid is 82:18, which is highly conducive to lower the blood cholesterol. Soy food contains most of the desirable characteristics therefore; it may be treated as consummate functional food.Soybean also contains many minor substances, which are biologically active non nutritive components known as “phytochemicals”, “isoflavones” or “phytoestrogen” also known as coumesterol or “plant estrogen” very similar to that of female sex hormone estrogen) with very weak activity of estrogen, which offers unique health benefits.Soybean is a good source of niacin, riboflavin, iron, potassium, calcium, magnesium, and phosphorous with several fat and water soluble vitamin Bcomplex (Tripathi, 2005). Soybeans are often called the “miracle crop.” They are the world’s foremost provider of vegetable protein and oil. The bushy, green soybean plant is a legume related to peas, groundnuts (peanuts) and alfalfa.Soybeans are included in the category of oilseed, which is a generic reference to crops with seeds that can produce edible and/or non-edible oil in economic quantities. The most versatile of the world’s major crops, soybeans can be grown in a wider variety of soil and climatic conditions than any other major world crop. Consequently, soybeans are the most widely grown oilseed in the world. In the last 20 years, scientists have learned how to extract a much wider variety of byproducts from soybeans that are proving beneficial in animal feed, human food and industrial applications.Rats have a long history in medical research. They were the first mammalian species specifically domesticated to be used in the laboratory. They are thought to have originated in some parts of Asia; Rattusrattus was well established in Europe by 1100 A.D., with Rattus norvegicus commonly found in Europe in the 1700s. By the 1800s, these animals were used for neuro-anatomy studies in the United States and in Europe. It was in the late 1800s and early 1900s that individual stocks and strains had their beginnings (Animals in research, 2013; Sengupta, 2013).The success of the rat in research today has been linked to the Wistar Institute in the United States of America, when in 1906, the institute developed the Wistar albino strain (Rattus norvegicus) (The Wistar Institute: History, 2007). Currently, there are 117 albino strains of the laboratory rat, all of which can be traced genetically back to the one rat, likely to have arisen as a mutation from a hooded (piebald) rat strain (Animals in research, 2013).1.2   STATEMENT OF THE PROBLEMSoy bean is a legume that is very rich in vitamins and when mixed with other grains to be used as a complementary food can also improve hormonal functionality. But in a situation of determining the effect of it in hormonal growth of rat becomes a little bit problematic in the sense that there might be inadequate instrument for the experiment to be carried out, secondly might be that there might be no competent hands in the laboratory to carry out the experiment. Thirdly could be that there are no good laboratory set up to carry out all the necessary experiment needed. Finally, there have been several research carried out on the effect of soya bean but there have been no single research carried out on the effect of soy groundnut mixed grain complementary food on the level of selected food on the growth of hormones in male weaning wistars rat.1.3   AIMS AND OBJECTIVES OF STUDY        The main aim of the study is to examine the effect of soy groundnut mixed grain complementary food on the levels of selected growth hormones in male weaning wistars rat. Other specific objectives of the study include:1.         to determine the effect of soy groundnut on the growth hormones in male weaning wistars rat.2.         to determine the factors affecting the growth hormones of male weaning wistars rat.3.         to determine the extent to which soy groundnut affects the growth hormones in male weaning wistars rats.4.         to proffer possible solutions to the problems.1.4      RESEARCH QUESTIONS1   What is the effect of soy groundnut on the growth hormones in male weaning wistars rat?2       What are the factors affecting the growth hormones of male weaning wistars rat?3       What is the extent to which soy groundnut affects the growth hormones in male weaning wistars rats?4       What are the possible solutions to the problems?1.5   STATEMENT OF RESEARCH HYPOTHESISHypothesis 1H0:   Soy groundnut mixed grain complementary food has no significant effect on growth hormones in male wistars rats.H1:       Soy groundnut mixed grain complementary food has a significant effect on growth hormones in male wistars rats.Hypothesis 2H0: There is no significant difference in growth hormones in male weaning wister rats.H1: There is significant difference in growth hormones in male weaning wister rats1.6   SIGNIFICANCE OF STUDY        The study on the effect of soy groundnut mixed grain complementary food on the levels of selected growth hormones in male weaning wistars rats will be of immense benefit to the entire laboratories in the sense that it will enable laboratory scientist to examine the effect of complementary food on the growth hormones of wistars rat; due to the fact that most laboratory experiment is normally carried out on rat before carrying it out on human, it will therefore give them the result or the reaction they seek in rat before human; this will give them a clue of what will happen to human beings when they eventually take soy groundnut mixed grain complementary food. Finally, the study will contribute to the body of existing literature and knowledge to this field of study and basis for further research.  1.7   SCOPE OF STUDYThe study on the effect of soy groundnut mixed grain complementary food is limited to growth hormones in male weaning wistars rat.1.8   LIMITATION OF STUDYFinancial constraint- Insufficient fund tends to impede the efficiency of the researcher in sourcing for the relevant materials, literature or information and in the process of data collection (internet, questionnaire and interview).Time constraint- The researcher will simultaneously engage in this study with other academic work. This consequently will cut down on the time devoted for the research work.1.9   DEFINITION OF TERMSEffectA change which is a result or consequence of an action or other cause.Soy GroundnutThe soybean, or soya bean, is a species of legume native to East Asia, widely grown for its edible bean, which has numerous uses.Growth HormonesAlso known as somatotropin, is a peptide hormone that stimulates growth, cell reproduction, and cell regeneration in humans and other animals.Wistars Rat This is a laboratory rat with its red eyes and white furs is an iconic model organism for research in a variety of fields.