DETERMINATION OF DEGRADING ABILITY OF FUNGI ISOLATED FROM HYDROCARBON POLLUTED SOIL ON CRUDE OIL USING GAS CHROMATOGRAPHY

Project Abstract

This study scientifically justifies the use of fungi to degrade crude oil. This project was therefore carried out to determine the degrading ability of fungi isolated from crude oil-contaminated soil samples using gas chromatography. Adapted microbial communities have higher proportions of hydrocarbon degraders that can respond to the presence of hydrocarbon pollutants. The measurement of biodegradation rates under favorable laboratory conditions using 14C-labelled hexadecane has led to the estimation that as much as 0.5 – 60 g oil/m3 seawater convert to carbon dioxide, depending on temperature and mineral nutrient conditions. The principal forces limiting the biodegradation of polluting petroleum in the sea are the resistant and toxic components of oil itself, low water temperatures, scarcity of mineral nutrients (especially nitrogen and phosphorous), the exhaustion of dissolved oxygen and in previously unpolluted areas, the scarcity of hydrocarbon-degrading microorganisms (Atlas, 2002)

Project Overview

1.0 INTRODUCTIONCrude oils are composed of mixtures of paraffin, alicyclic and aromatic hydrocarbons. Microbial communities exposed to hydrocarbons become adapted, exhibiting selective enrichment and genetic changes resulting in increased proportions of hydrocarbon-degrading bacteria and bacterial plasmids encoding hydrocarbon catabolic genes (Leahy and Colwell, 2004). Adapted microbial communities have higher proportions of hydrocarbon degraders that can respond to the presence of hydrocarbon pollutants. The measurement of biodegradation rates under favorable laboratory conditions using 14C-labelled hexadecane has led to the estimation that as much as 0.5 – 60 g oil/m3 seawater convert to carbon dioxide, depending on temperature and mineral nutrient conditions. The principal forces limiting the biodegradation of polluting petroleum in the sea are the resistant and toxic components of oil itself, low water temperatures, scarcity of mineral nutrients (especially nitrogen and phosphorous), the exhaustion of dissolved oxygen and in previously unpolluted areas, the scarcity of hydrocarbon-degrading microorganisms (Atlas, 2002).Low winter temperature can limit rates of hydrocarbon biodegradation increasing resident time of oil pollutant(Bodennec et al., 2007).Bio-degradation is nature’s way of recycling wastes, or breaking down organic matter into nutrients that can be used and reused by other organisms. In the microbiological sense,”bio-degradation” means that the decaying of all organic materials is carried out by a huge assortment of life forms comprising mainly bacteria and fungi, and other organisms. This pivotal,natural, biologically mediated process is the one that transforms hazardous toxic chemicals intonon-toxic or less toxic substances. In a very broad sense, in nature, there is no waste because almost everything gets recycled. In addition, the secondary metabolites, intermediary molecule or any ‘waste products’ from one organism become the food/nutrient source(s) for others,providing nourishment and energy while they are further working-on/breaking down the so called waste organic matter. Some organic materials will break down much faster than others, but all will eventually decay.By harnessing microbial communities, the natural “forces” of biodegradation, reduction of wastes and clean up of some types of environmental contaminants can be achieved. There are several reasons for which this process is better than chemical or physical processes. For example, this process directly degrades contaminants rather than merely transforming them from one form to the other, employ metabolic degradation pathways that can terminate with benign terminal products like CO2 and water, derive energy directly from the contaminants themselves, and can be used in situ to minimize the disturbances usually associated with chemical treatment at the clean-up sites. Biological degradation of organic compounds may be considered an economical tool for remediating hazardous waste-contaminated environments. While some environments may be too severely contaminated for initial in situ treatment to be effective, most contaminated media will use some form of biological degradation in the final treatment phase.Diverse groups of fungi have been isolated from oil contaminated environments and/or have been shown to degrade hydrocarbons in the laboratory. Microbial degradation is the major mechanism for the elimination of spilled oil from the environment ( Atlas, 2000.). In this study, crude oil-contaminated soil samples areas in delta state were examined with the aim of isolating fungi with high crude oil degrading potentials.1.1 Justification of the studyVarious studies have identified some microorganisms to be able to degrade crude oil. The degrading ability of these microorganisms have been determined using different methods such as gas chromatography and mass spectrometry (GC/MS), gas chromatography (GC), turbidimetry, titrimetry e.t.c. Biodegradation of crude oil is majorly carried out by bacteria and fungi. This study scientifically justifies the use of fungi to degrade crude oil. This project was therefore carried out to determine the degrading ability of fungi isolated from crude oil-contaminated soil samples using gas chromatography.1.2 Objective of the studyThe specific objectives of the study are to :I. Isolate and identify fungi from crude oil-contaminated soil sample.II. Screen the isolates for bio-degradative abilities.III. Assessment of the degrading abilities of the fungi isolates by gas chromatography