In vitro regeneration of treculia africana decne. from embryo explants on the media of murashige and skoog (ms) and gamborg et al. (b5)
Table Of Contents
Chapter ONE
1.1 Introduction1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms
Chapter TWO
2.1 Overview of Plant Regeneration2.2 Historical Development of Plant Tissue Culture
2.3 Importance of In Vitro Plant Regeneration
2.4 Factors Affecting Plant Regeneration Efficiency
2.5 Types of Plant Regeneration Pathways
2.6 Role of Plant Growth Regulators in Regeneration
2.7 Techniques for In Vitro Regeneration
2.8 Recent Advances in Plant Regeneration Research
2.9 Comparison of Different Regeneration Protocols
2.10 Challenges in Plant Regeneration Studies
Chapter THREE
3.1 Research Design and Methodology3.2 Selection of Plant Material
3.3 Sterilization Techniques for Explants
3.4 Culture Media Preparation
3.5 Explant Inoculation and Incubation
3.6 Subculture and Maintenance of Cultures
3.7 Data Collection and Analysis Methods
3.8 Statistical Tools for Data Interpretation
Chapter FOUR
4.1 Analysis of Regeneration Frequency4.2 Evaluation of Growth Parameters
4.3 Histological Studies of Regenerated Plants
4.4 Molecular Analysis of Regenerated Plants
4.5 Comparison with Control Plants
4.6 Discussion on Regeneration Efficiency
4.7 Factors Influencing Regeneration Success
4.8 Implications for Further Research
Chapter FIVE
5.1 Summary of Findings5.2 Conclusion and Interpretation
5.3 Contribution to Existing Knowledge
5.4 Recommendations for Future Research
5.5 Practical Applications of the Study
Project Abstract
The effect of three different concentrations of sucrose namely 2, 3 and 4% were investigated on the in vitro regeneration of embryo explants of Treculia africana Decne. on the media of Murashige and Skoog (MS) (1962) and Gamborg et al. (B5) (1968) respectively without any growth regulator. The experimental design was a 2 x 5 factorial in a completely randomized design with each treatment consisting of ten replicates. Results showed that while both media including control supported the in vitro regeneration of T. africana embryo explants, B5 medium was found to be significantly superior (P≤0.05) to MS medium in all the growth parameters studied. B5 medium at 4% sucrose elicited the best response in all the growth parameters determined while control gave the least response. The protocol reported here can be used for large scale propagation of true-to-type T. africana plants within a short time for the purpose of improvement through genetic transformation (mutagenesis) and the development of a viable conservation programme.
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