Project Abstract

Abstract
In recent years, microRNAs (miRNAs) have emerged as key players in the regulation of gene expression, with implications in various biological processes and diseases, including cancer. This research project aims to investigate the role of miRNAs in regulating gene expression in cancer cells, with a focus on understanding how dysregulation of miRNAs contributes to cancer development and progression. Through a comprehensive literature review and experimental studies, this project seeks to elucidate the mechanisms by which miRNAs interact with target genes to influence cancer cell behavior. Chapter One provides an introduction to the research topic, discussing the background of miRNAs, the problem statement regarding their involvement in cancer, the objectives of the study, limitations and scope of the research, the significance of the study, the structure of the research, and definitions of key terms. Chapter Two presents a detailed literature review on miRNAs and their role in cancer, covering topics such as miRNA biogenesis, mechanisms of miRNA-mediated gene regulation, and the dysregulation of miRNAs in various cancer types. Chapter Three outlines the research methodology, including the experimental design, cell culture techniques, miRNA profiling methods, bioinformatics analysis, and statistical approaches used to study miRNA regulation of gene expression in cancer cells. This chapter also discusses the validation of miRNA-target interactions and functional studies to assess the impact of miRNAs on cancer cell phenotypes. Chapter Four presents the findings of the research, detailing the specific miRNAs identified as regulators of gene expression in cancer cells, their target genes, and the pathways affected by miRNA dysregulation. The chapter also includes a comprehensive discussion of the results, highlighting the implications of the findings for cancer biology and potential therapeutic targets. Chapter Five concludes the research project, summarizing the key findings, discussing their significance in the context of existing literature, and proposing future directions for further research in this field. Overall, this research project aims to contribute to the understanding of how miRNAs regulate gene expression in cancer cells and provide insights into novel strategies for cancer treatment and personalized medicine. Keywords microRNAs, gene expression, cancer cells, regulation, dysregulation, biogenesis, mechanisms, targets, pathways, therapeutic targets, personalized medicine.

Project Overview

The project titled "Investigating the role of microRNAs in regulating gene expression in cancer cells" aims to delve into the intricate mechanisms by which microRNAs influence gene expression in cancer cells. MicroRNAs are small non-coding RNA molecules that play a crucial role in post-transcriptional gene regulation by binding to specific target messenger RNAs (mRNAs) and either inhibiting their translation or promoting their degradation. In the context of cancer, dysregulation of microRNAs has been implicated in various aspects of tumorigenesis, including cell proliferation, apoptosis, metastasis, and drug resistance. The research will focus on elucidating how specific microRNAs modulate the expression of key genes involved in cancer development and progression. By identifying the target mRNAs of these microRNAs and studying their downstream effects on cellular processes, the study aims to provide insights into the molecular mechanisms underlying cancer pathogenesis. Additionally, the project will investigate the potential use of microRNAs as diagnostic biomarkers and therapeutic targets in various types of cancer. Through a combination of experimental approaches, including cell culture studies, molecular biology techniques, and bioinformatic analysis, the research will aim to uncover novel interactions between microRNAs and target genes in cancer cells. By integrating data from both in vitro and in vivo experiments, the study seeks to provide a comprehensive understanding of the regulatory networks controlled by microRNAs in cancer. Overall, this research project holds significant promise for advancing our understanding of the role of microRNAs in cancer biology and may pave the way for the development of innovative diagnostic and therapeutic strategies for cancer treatment. By shedding light on the complex interplay between microRNAs and gene expression in cancer cells, the study aims to contribute to the growing body of knowledge aimed at combating this devastating disease.