Home / Biochemistry / 1.1 BACKGROUND OF STUDY WHAT IS WATER? Water is the most abundant liquid on earth it covers three quarters of the earth surface. Oceans cover wsout 72% of the earth and the average depth is 2.5 miles. The oceans are the source of 97% of all water. The rest consist of 2.1% in glaciers, 0.6% fresh water in lakes and rivers 0.6% ground waters and 0.1% in brine well and brackish waters. Water is the major components of living things for example, the water content of human adult is 70% the same proportion as the earths surface. Water is a liquid, which is colourless, odourless and tasteless. Human activities and settlements hinge on the availability of water. In man, three quarters of the fluid in him are made of water, water forms the essential medium in which the chemical reactions of his cells processed. IMPORTANCE OF WATER Our physical biological environment play important role on water. As a vapour, it absorbs radiation to influence the heat balance and temperature of the environment thereby bringing moisture to the containment through the water cycle. Human activities and settlement also generate important role on water availability which is used as a medium into which man disposes his wastes which could be industrial domestic or agricultural. Water is so important that it is involved in almost every activity in man. 1.2 STATEMENT OF PROBLEM The research her realised that some of the pure water sachets and boreholes water have a lot of problems in terms of not using the adequate or sufficient PH value recommended by the NAFDAC. As this has direct effect with the general interest of the consumers positively or negatively which influences the all round achievement in pure water sachets and borehole water. So in doing this, the following questions call for attention. 1. Are pure water sachet companies in Benin City have too much acid content? 2. Are the borehole water have too much acid content 3. Is effluent from sewage plant properly treated? 4. Are all the pure water sachet companies registered with the NAFDAC 5. Does drinking water have taste, odour and colour? 1.3 PURPOSE OF THE STUDY The relevance of this research work is to examine and make a report on PH value of sachet water samples and borehole water sample in Benin City. Some of the drinking water has already been contaminated, that is to say, the packaging and that of the nylons used to package the treated water are not properly checked. This research work is also aim to determine to what level pure water sachet and borehole water contained too acid and also to check whether all the pure water sachet companies are registered with NAFDAC (national Agency for food and Drug Administration Company). 1.4 AIMS AND OBJECTIVE OF THE STUDY The aims and objective of this study is to provide possible solutions that will sustain pure water sachet companies and borehole water in Benin City, Edo State. 1. To ensure consumer buy pure water sachet registered with NAFDAC. 2. To encourage consumers to report to the NAFDAC, if they see pure water sachet not registered being sold for drinking. 3. To ensure pure water companies, the PH value of the water produced for consumption are within the standard recommended by the WHO for drinking water. 4. To check packaging and ensuring that the nylons used to package the treated water do not contaminate the water put into it or give it some unwanted odour or taste. 5. To ensure that, the best chemical is been used during the treatment of water. 6. To ensure that sewage water be treated carefully. 7. To sanction all the pure water companies not registered with NAFDAC. 1.5 SIGNIFICANCE OF STUDY The researcher hopes what this research work will go along way for the society in developing the positive effects and thinking towards the determination of PH value of pure water sachets and borehole sample. The research work is considered on believe to make the treatment more inclined to pure water sachet and bore hole water and greatly helps the companies in formulating a well planned programmer that will inculcate the right attitude towards the PH value of pure water sachet and borehole water in Benin City. The research study is believed will make reforms of the present greatly increasingly emphasis on providing the treatment equipment for the pure water sachet and borehole water. The researcher study is to make all the pure water sachets and borehole water to be properly checked before drinking in order to improve good health. Finally, the research work is believed to make an effort so that all the effluence from sewage plant is treated with chloride before drinking. 1.6 HYPOTHESIS 1. Some of the pure water sachet companies in Benin City have too much acid content. 2. Some of the bore hole water have too much acid content 3. The effluent from sewage plant is not properly treated. 4. Some of the pure water sachet companies is not registered with NAFDAC. 5. Some drinking water has taste, odour and colour. 1.7 SCOPE OF STUDY This research study covers five selected pure water sachets companies and five of many different type of borehole water were looked into to see if there PH is in line with the recommendation for drinking water from the world health organisation WHO standard in respect to PH of each water sample. PURE WATER SAMPLE 1. Uniben pure water 2. Maria pure water 3. Ages pure water 4. Faith mark pure water 5. Uncle Sam pure water BOREHOLE SAMPLE 1. No 8 Edo Street off technical road 2. Black 25 technical estate 3. No 46 Jonathan Akpobaries street 4. No 3 15th Street off estate junction 5. No. 8 Otahoigbogie Street, off Adolor College. 1.8 DEFINITION OF TERMS Water: This is a liquid without colour, taste or odour that falls as rain, is in lake, rivers, and seas, is used for drinking, washing and so on. Pollution: It is a change in physical, chemical, or Biological conditions in the environment of the air. Water or land that can harmfully affect health, survival, or activities of human or other living organisms. Acid: These are the compounds that produces hydrogen ion (H+) as the only positive ions. Base: It is a compound that produces hydroxide ions (H-) in aqueous solutions. PH: This is defined as the negative logarithm to base ten of the hydrogen ion concentration.

1.1 BACKGROUND OF STUDY WHAT IS WATER? Water is the most abundant liquid on earth it covers three quarters of the earth surface. Oceans cover wsout 72% of the earth and the average depth is 2.5 miles. The oceans are the source of 97% of all water. The rest consist of 2.1% in glaciers, 0.6% fresh water in lakes and rivers 0.6% ground waters and 0.1% in brine well and brackish waters. Water is the major components of living things for example, the water content of human adult is 70% the same proportion as the earths surface. Water is a liquid, which is colourless, odourless and tasteless. Human activities and settlements hinge on the availability of water. In man, three quarters of the fluid in him are made of water, water forms the essential medium in which the chemical reactions of his cells processed. IMPORTANCE OF WATER Our physical biological environment play important role on water. As a vapour, it absorbs radiation to influence the heat balance and temperature of the environment thereby bringing moisture to the containment through the water cycle. Human activities and settlement also generate important role on water availability which is used as a medium into which man disposes his wastes which could be industrial domestic or agricultural. Water is so important that it is involved in almost every activity in man. 1.2 STATEMENT OF PROBLEM The research her realised that some of the pure water sachets and boreholes water have a lot of problems in terms of not using the adequate or sufficient PH value recommended by the NAFDAC. As this has direct effect with the general interest of the consumers positively or negatively which influences the all round achievement in pure water sachets and borehole water. So in doing this, the following questions call for attention. 1. Are pure water sachet companies in Benin City have too much acid content? 2. Are the borehole water have too much acid content 3. Is effluent from sewage plant properly treated? 4. Are all the pure water sachet companies registered with the NAFDAC 5. Does drinking water have taste, odour and colour? 1.3 PURPOSE OF THE STUDY The relevance of this research work is to examine and make a report on PH value of sachet water samples and borehole water sample in Benin City. Some of the drinking water has already been contaminated, that is to say, the packaging and that of the nylons used to package the treated water are not properly checked. This research work is also aim to determine to what level pure water sachet and borehole water contained too acid and also to check whether all the pure water sachet companies are registered with NAFDAC (national Agency for food and Drug Administration Company). 1.4 AIMS AND OBJECTIVE OF THE STUDY The aims and objective of this study is to provide possible solutions that will sustain pure water sachet companies and borehole water in Benin City, Edo State. 1. To ensure consumer buy pure water sachet registered with NAFDAC. 2. To encourage consumers to report to the NAFDAC, if they see pure water sachet not registered being sold for drinking. 3. To ensure pure water companies, the PH value of the water produced for consumption are within the standard recommended by the WHO for drinking water. 4. To check packaging and ensuring that the nylons used to package the treated water do not contaminate the water put into it or give it some unwanted odour or taste. 5. To ensure that, the best chemical is been used during the treatment of water. 6. To ensure that sewage water be treated carefully. 7. To sanction all the pure water companies not registered with NAFDAC. 1.5 SIGNIFICANCE OF STUDY The researcher hopes what this research work will go along way for the society in developing the positive effects and thinking towards the determination of PH value of pure water sachets and borehole sample. The research work is considered on believe to make the treatment more inclined to pure water sachet and bore hole water and greatly helps the companies in formulating a well planned programmer that will inculcate the right attitude towards the PH value of pure water sachet and borehole water in Benin City. The research study is believed will make reforms of the present greatly increasingly emphasis on providing the treatment equipment for the pure water sachet and borehole water. The researcher study is to make all the pure water sachets and borehole water to be properly checked before drinking in order to improve good health. Finally, the research work is believed to make an effort so that all the effluence from sewage plant is treated with chloride before drinking. 1.6 HYPOTHESIS 1. Some of the pure water sachet companies in Benin City have too much acid content. 2. Some of the bore hole water have too much acid content 3. The effluent from sewage plant is not properly treated. 4. Some of the pure water sachet companies is not registered with NAFDAC. 5. Some drinking water has taste, odour and colour. 1.7 SCOPE OF STUDY This research study covers five selected pure water sachets companies and five of many different type of borehole water were looked into to see if there PH is in line with the recommendation for drinking water from the world health organisation WHO standard in respect to PH of each water sample. PURE WATER SAMPLE 1. Uniben pure water 2. Maria pure water 3. Ages pure water 4. Faith mark pure water 5. Uncle Sam pure water BOREHOLE SAMPLE 1. No 8 Edo Street off technical road 2. Black 25 technical estate 3. No 46 Jonathan Akpobaries street 4. No 3 15th Street off estate junction 5. No. 8 Otahoigbogie Street, off Adolor College. 1.8 DEFINITION OF TERMS Water: This is a liquid without colour, taste or odour that falls as rain, is in lake, rivers, and seas, is used for drinking, washing and so on. Pollution: It is a change in physical, chemical, or Biological conditions in the environment of the air. Water or land that can harmfully affect health, survival, or activities of human or other living organisms. Acid: These are the compounds that produces hydrogen ion (H+) as the only positive ions. Base: It is a compound that produces hydroxide ions (H-) in aqueous solutions. PH: This is defined as the negative logarithm to base ten of the hydrogen ion concentration.

 

Table Of Contents


Project Abstract

Abstract
Water is a crucial component of life on earth, with the majority of the planet's surface covered by oceans. Human activities and settlements heavily rely on the availability of water, with water playing a vital role in various biological and environmental processes. However, concerns have been raised regarding the quality of water from pure water sachets and boreholes, particularly in Benin City. This study aims to investigate issues such as the pH levels of water samples, potential acid content in pure water sachets and borehole water, proper treatment of sewage plant effluents, registration of pure water companies with regulatory bodies like NAFDAC, and the presence of taste, odor, and color in drinking water. The research seeks to address these problems by ensuring that water sources meet recommended standards, encouraging consumer awareness and reporting of substandard products, and promoting the use of appropriate treatment methods to enhance water quality. By focusing on selected pure water sachet companies and borehole water sources in Benin City, this study aims to contribute to improving water quality, protecting public health, and enhancing regulatory compliance within the water supply industry. The significance of this research lies in its potential to drive positive changes in the treatment and distribution of pure water sachets and borehole water, ultimately benefiting both the companies involved and the general public. Through careful examination and monitoring, this study aims to promote a culture of accountability, quality control, and safety in water provision, ultimately leading to healthier and safer drinking water for consumers.

Project Overview

2.1 MATERIALS

2.1.1 PLANT MATERIALS FOR ANALYSIS

Fresh mature plants of Acalypha wilkesiana were collected from the natural habitat in October within Ambrose Alli University, Ekpoma, Edo State, Nigeria. The plant was authenticated in the Department of Botany, Ambrose Alli University, Ekpoma, Edo State, Nigeria. The leaves were picked, washed in distilled water air dried and weighed. The weight of the leaf was 0.1kg. The dried leaves were pounded to powdered form using a mortar and pestle. The leaf powder was suspended in methanol for 24 hours before filtering with a white filter cloth and boiled for 30 minutes. The resulting methanol extract was allowed to attain room temperature and was evaporated to dryness using a water bath as a source of heat. The percentage yield of the extract was 6.41% (w/v). The pasty residue was used to prepare a standardized solution of the leaf extract in calculated amount of distilled water with standardized concentration of 0.46 g/ml. The standardized solution (the stock solution) was stored airtight in plastic bottles and kept frozen.

2.1.2 LABORATORY ANIMALS

Exactly twenty four male albino rats weighing 148 ± 30g obtained from the Animal Holding Unit of the Institute of Agricultural Research and Training (IART) Apata-Ibadan, Nigeria were used for the study. The rats were fed with Guinea grower mash (product of bendel feeds and flour mills Ltd, Ewu, Edo state, Nigeria). The rats were grouped into four (4) groups, each groups consisting of six (6) rats.

2.2   INSTRUMENTS USED

The instruments used for this analysis include;

Beam weigh balance

Electronic weigh balance

2.3 METHODS

2.3.1 EXPERIMENTAL ANIMALS AND PROCEDURE

The Twenty Four Male albino rats were randomly grouped into four  consisting of six rats per group. The rats were kept in cages made of wooden frames and metal iron and were fed ad libitum (access to food and water for 24 hours each day). The rats were fed with guinea grower mash and tap water with 12 hours light and dark cycle. The cages were cleaned every morning and disinfected every day. The rats were allowed to acclimatize for seven days before extract administration was commenced. Calculated amount of methanolic leaf extract of Acalypha wilkesiana were constituted in distilled water from the stock solution to give doses of 500,500 and 1000 mg/kg body weight of paracetamol, vitamin C and leaf extract respectively and administered to the various groups as illustrated below;

GROUP A: Control received 1 ml of distilled water

GROUP B: Received 500 mg/kg body weight of paracetamol

GROUP C: Received 500 mg/kg paracetamol and vitamin C

GROUP D: Received 500 mg/kg paracetamol and 1000 mg/kg leaf extract.

Prior to the administration of methanolic leaf extract of Acalypha wilkesiana at every interval of seven days, the body weights of the animals were recorded. Administration of methanolic leaf extract of Acalypha wilkesiana was performed orally once daily between 8:30am ± 30 minutes, using metal cannula attached to a 2ml syringe prior to the administration of methanolic leaf extract of Acalypha Wilkesiana at every interval of seven days, the body weights of the animals were recorded.


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