Life cycle assessment of the environmental impact of biosurfactant production from oil waste by a diculture of azotobacter vinelandii and pseudomonas sp.
Table Of Contents
Chapter ONE
1.1 Introduction1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms
Chapter TWO
2.1 Overview of Biosurfactants2.2 Environmental Impact of Biosurfactant Production
2.3 Azotobacter Vinelandii: Characteristics and Applications
2.4 Pseudomonas sp.: Characteristics and Applications
2.5 Microbial Diculture in Biosurfactant Production
2.6 Oil Waste as a Substrate for Biosurfactant Production
2.7 Methods of Biosurfactant Production
2.8 Market Trends and Applications of Biosurfactants
2.9 Sustainable Production Practices
2.10 Global Initiatives for Environmental Sustainability
Chapter THREE
3.1 Research Design and Methodology3.2 Selection of Microbial Strains
3.3 Cultivation and Growth Conditions
3.4 Biosurfactant Extraction Methods
3.5 Analytical Techniques for Characterization
3.6 Environmental Impact Assessment Methods
3.7 Data Collection and Analysis
3.8 Statistical Tools and Software Used
Chapter FOUR
4.1 Analysis of Biosurfactant Production Efficiency4.2 Characterization of Biosurfactants Produced
4.3 Environmental Impact Assessment Results
4.4 Comparison with Conventional Surfactants
4.5 Economic Analysis of Production Process
4.6 Discussion on Sustainability and Scalability
4.7 Recommendations for Process Optimization
4.8 Future Research Directions
Chapter FIVE
5.1 Summary of Findings5.2 Conclusions Drawn from the Study
5.3 Implications for Environmental Sustainability
5.4 Recommendations for Industry and Policy
5.5 Reflection on Research Process
5.6 Contribution to Scientific Knowledge
5.7 Limitations and Areas for Future Research
5.8 Closing Remarks and Acknowledgments
Project Abstract
During the bioprocess of the biosurfactant production (by the consortium), the volume of CO2 evolved was 28.23 ± 5.08 cm3, which is equivalent to 0.056 ± 0.01g CO2 per 100 ml of broth. In terms of gCO2/1000Kg biosurfactant, this gave a value of 4545 ± 817.93g CO2 in this bioprocess. Following from above, the life cycle impact assessment (LCIA) of biosurfactant production by this consortium, based on global warming potential (GWP) was 0.046 tonnes/1000Kg biosurfactant. Other impact values calculated for acidification and eutrophication potentials were 0.008 tonnes / 1000 Kg and 0.0014 tonnes/1000Kg of biosurfactant. These values were considered insufficient in terms of environmental pollution when compared with the regular methods of surfactants production. In this work also, the consortium of Pseudomonas sp/Azotobacter vinelandii produced 1.22 ± 0.04 mg biosurfactant per 100ml of cell – free broth. However, the individual organisms (Pseudomonas sp. and Azotobacter vinelandii) produced 1.03 ± 0.02 and 0.08 ± 0.001 mg of biosurfactants per 100 ml cell – free broth respectively. The microbial growth kinetics during the production of the biosurfactant by the consortium gave a specific maximum growth (µmax) of 1.306 ± 0.201 hr-1), a saturation constant (Ks) of 0.017 ± 0.007 mg/l, an Inhibition constant (Ki) of 121.83 ± 21.18 mg/land a Death constant (Kd) of 0.017 ± 0.006 mg/l. These values when compared with those of individual organisms shows that using a consortium for the bioprocess is more sustainable.
Project Overview
Blazingprojects Mobile App
📚 Over 50,000 Project Materials
📱 100% Offline: No internet needed
📝 Over 98 Departments
🔍 Software coding and Machine construction
🎓 Postgraduate/Undergraduate Research works
📥 Instant Whatsapp/Email Delivery
Related Research
Exploring the Role of MicroRNAs in Cancer Progression and Therapeutic Resistance...
The project titled "Exploring the Role of MicroRNAs in Cancer Progression and Therapeutic Resistance" aims to investigate the intricate involvement of...
Exploring the Role of MicroRNAs in Cancer Development and Progression...
The project topic, "Exploring the Role of MicroRNAs in Cancer Development and Progression," focuses on investigating the intricate involvement of micr...
Exploring the Role of MicroRNAs in Cancer Progression and Therapeutic Potential...
The project topic, "Exploring the Role of MicroRNAs in Cancer Progression and Therapeutic Potential," delves into the intricate world of microRNAs and...
Investigating the role of microRNAs in regulating gene expression in cancer cells....
The project titled "Investigating the role of microRNAs in regulating gene expression in cancer cells" aims to delve into the intricate mechanisms by ...
Exploring the Role of Epigenetics in Cancer Development and Therapeutic Approaches...
The project titled "Exploring the Role of Epigenetics in Cancer Development and Therapeutic Approaches" aims to investigate the intricate relationship...
Identification and Characterization of Novel Enzymes Involved in Plant Secondary Met...
The project on "Identification and Characterization of Novel Enzymes Involved in Plant Secondary Metabolite Biosynthesis" aims to explore the intricat...
Exploring the Role of Gut Microbiota in Human Health and Disease...
The project topic, "Exploring the Role of Gut Microbiota in Human Health and Disease," delves into the intricate relationship between gut microbiota a...
Investigating the Effects of Different pH Levels on Enzyme Activity in Biological Sy...
The project topic, "Investigating the Effects of Different pH Levels on Enzyme Activity in Biological Systems," focuses on exploring how varying pH le...
Investigating the role of epigenetic modifications in cancer development and progres...
The project "Investigating the role of epigenetic modifications in cancer development and progression" aims to explore the intricate relationship betw...
