Project Abstract

Cancer is a devastating disease that affects millions of people worldwide, posing a significant challenge to the medical community. While genetic mutations have long been recognized as a primary driver of cancer development, emerging evidence suggests that epigenetic modifications also play a crucial role in the progression and metastasis of this disease. This project aims to delve into the intricate relationship between epigenetic changes and cancer progression, with the ultimate goal of uncovering new avenues for targeted therapies and improved patient outcomes. Epigenetics, the study of heritable changes in gene expression that do not involve alterations in the DNA sequence, has gained increasing attention in the field of cancer research. Epigenetic mechanisms, such as DNA methylation, histone modifications, and chromatin remodeling, can significantly influence gene expression patterns, leading to the activation or silencing of key oncogenes and tumor suppressor genes. Understanding the dynamic interplay between these epigenetic alterations and the molecular pathways involved in cancer progression is essential for developing more effective treatment strategies. This project will employ a multifaceted approach to explore the role of epigenetic modifications in cancer progression. First, we will conduct a comprehensive literature review to synthesize the current understanding of the relationship between epigenetics and cancer progression, identifying the key epigenetic mechanisms that contribute to the development and metastasis of various cancer types. This will provide a solid foundation for the subsequent experimental investigations. Next, we will utilize cutting-edge genomic and epigenomic profiling techniques, such as whole-genome bisulfite sequencing and chromatin immunoprecipitation sequencing (ChIP-seq), to generate high-resolution maps of DNA methylation patterns and histone modifications in a diverse set of cancer cell lines and patient-derived samples. By comparing the epigenetic landscapes of cancer cells at different stages of progression, we aim to identify specific epigenetic signatures that distinguish aggressive, metastatic phenotypes from less invasive counterparts. To further elucidate the functional implications of these epigenetic alterations, we will employ in vitro cell culture models and in vivo animal studies. Through targeted manipulation of key epigenetic regulators, such as DNA methyltransferases and histone-modifying enzymes, we will investigate the impact of epigenetic changes on cellular proliferation, migration, invasion, and drug sensitivity. These experiments will shed light on the mechanistic underpinnings of how epigenetic modifications contribute to cancer progression and metastasis. Importantly, this project will also explore the potential of epigenetic biomarkers for the early detection and prognostic stratification of cancer patients. By identifying novel epigenetic signatures associated with aggressive disease phenotypes, we aim to develop non-invasive liquid biopsy-based assays that can aid in the early diagnosis and personalized management of cancer patients. The findings from this comprehensive study will advance our understanding of the complex interplay between epigenetics and cancer progression, paving the way for the development of more effective, targeted therapies and improved patient outcomes. By unraveling the epigenetic mechanisms that drive cancer metastasis and resistance, this project has the potential to significantly impact the field of oncology and transform the way we approach the treatment and management of this devastating disease.

Project Overview