Home / Biochemistry / Isolation, characterisation and antimicrobial activity of bioactive constituents from the leaf extract of adenodolichos paniculatus (hua) hutch. & dalz (fabaceae)

Isolation, characterisation and antimicrobial activity of bioactive constituents from the leaf extract of adenodolichos paniculatus (hua) hutch. & dalz (fabaceae)

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Bioactive Constituents
2.2 Previous Studies on Adenodolichos Paniculatus
2.3 Extraction Methods for Bioactive Compounds
2.4 Characterization Techniques for Bioactive Compounds
2.5 Antimicrobial Activity of Bioactive Constituents
2.6 Role of Bioactive Compounds in Medicinal Plants
2.7 Biological Importance of Antimicrobial Properties
2.8 Mechanisms of Action of Bioactive Constituents
2.9 Synergistic Effects of Bioactive Compounds
2.10 Future Trends in Bioactive Compound Research

Chapter THREE

3.1 Research Design
3.2 Sampling Methods
3.3 Data Collection Procedures
3.4 Data Analysis Techniques
3.5 Ethical Considerations
3.6 Research Instrumentation
3.7 Statistical Analysis Approaches
3.8 Validity and Reliability Measures

Chapter FOUR

4.1 Overview of Research Findings
4.2 Bioactive Constituents Identified from Adenodolichos Paniculatus
4.3 Antimicrobial Activity Results
4.4 Comparison with Previous Studies
4.5 Interpretation of Results
4.6 Discussion on Mechanisms of Action
4.7 Implications of Findings
4.8 Recommendations for Further Research

Chapter FIVE

5.1 Summary of Findings
5.2 Conclusions Drawn from the Study
5.3 Contributions to Existing Literature
5.4 Practical Applications of Research
5.5 Limitations of the Study
5.6 Suggestions for Future Research
5.7 Conclusion and Final Remarks

Thesis Abstract

The plant Adenodolichos paniculatus which is used in ethno – medicine for the treatment of blennorrhoea, diarrhea, dysentery, small pox, heart burn and wound dressing was investigated for its chemical constituents and antimicrobial activity. The leaves of this plant were subjected to microwave assisted extraction to obtain n – hexane, chloroform, ethyl acetate and methanol extracts. The extracts were then subjected to preliminary phytochemical screening which revealed the presence of alkaloids, anthraquinones, flavonoids, reducing sugars, saponins, tannins and steroids/terpenes. Purification through various column chromatography, the ethyl acetate extract led to the isolation of compounds X1 (mixture of stigmasterol and β – sitosterol) and X2 (nonanoic acid) after spectral analysis IR, 1H NMR and 13C NMR. Determination of inhibitory activity (sensitivity test) using agar well diffusion method showed activity i.e. minimum inhibitory concentration (MIC) against Staphylococcus aureus, Salmonella typhi, and Candida albicans ranging from 12 – 50 mg/ml, Minimum Bactericidal Concentration/ Minimum Fungicidal Concentration (MBC/MFC) ranging from 25 – 50 mg/ml, which is found to be bacteriostatic against Salmonella typhi. Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Bacillus subtili showed no MBC (Bacteriostatic) for the methanol, ethyl acetate, chloroform and n –hexane extract.

Thesis Overview

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