Thesis Abstract

Abstract
MicroRNAs (miRNAs) are small non-coding RNA molecules that play crucial roles in post-transcriptional gene regulation. In recent years, research has shown that dysregulation of miRNAs is associated with various diseases, including cancer. This study aimed to analyze the role of miRNAs in cancer progression, with a focus on understanding their mechanisms of action and potential as diagnostic and therapeutic targets. Chapter One provides an introduction to the study, presenting the background and context of miRNAs in cancer progression. The problem statement highlights the significance of investigating miRNAs in cancer and the gaps in current knowledge. The objectives of the study include identifying key miRNAs involved in cancer progression and evaluating their potential as biomarkers or therapeutic targets. The limitations and scope of the study are also outlined, along with the significance of the research and the structure of the thesis. Definitions of key terms related to miRNAs and cancer are provided to aid in understanding the subsequent chapters. Chapter Two presents a comprehensive literature review on miRNAs and their roles in cancer. Ten key topics are discussed, including the biogenesis of miRNAs, their functions in cancer development and progression, and the diagnostic and therapeutic implications of miRNA dysregulation in cancer. Chapter Three details the research methodology employed in this study. The chapter includes descriptions of the study design, sample collection and processing methods, miRNA profiling techniques, bioinformatics analysis, and statistical methods used to analyze the data. The chapter also outlines the criteria for selecting miRNAs of interest and the rationale behind the experimental approach. Chapter Four presents the findings of the study, including the identification of dysregulated miRNAs in cancer samples, their putative target genes, and the pathways involved in cancer progression. The discussion delves into the implications of these findings for cancer diagnosis, prognosis, and treatment, highlighting the potential of miRNAs as biomarkers and therapeutic targets in cancer. Chapter Five concludes the thesis by summarizing the key findings, discussing their relevance to the field of cancer research, and suggesting directions for future studies. The study contributes to a better understanding of the role of miRNAs in cancer progression and underscores their potential as valuable tools in the diagnosis and treatment of cancer. In conclusion, this thesis provides a comprehensive analysis of the role of miRNAs in cancer progression, shedding light on their mechanisms of action and potential clinical applications. The findings of this study have implications for the development of novel diagnostic and therapeutic strategies for cancer management, emphasizing the importance of miRNAs as key players in cancer biology.

Thesis Overview

The project titled "Analysis of the role of microRNAs in cancer progression" aims to investigate the intricate role of microRNAs in the progression of cancer. MicroRNAs are small non-coding RNA molecules that play crucial roles in regulating gene expression at the post-transcriptional level. Dysregulation of microRNAs has been implicated in various diseases, including cancer, where they can act as oncogenes or tumor suppressors. The research will delve into the specific microRNAs that are known to be involved in different aspects of cancer progression, such as tumor initiation, growth, metastasis, and resistance to therapy. By understanding the mechanisms by which these microRNAs contribute to cancer development, novel therapeutic strategies can be developed to target them effectively. The study will employ a multidisciplinary approach, combining bioinformatics analysis, molecular biology techniques, and functional assays to unravel the complex network of interactions between microRNAs and their target genes in cancer cells. By elucidating these interactions, the research aims to identify key microRNAs that could serve as potential diagnostic and prognostic markers for cancer, as well as therapeutic targets for intervention. Furthermore, the project will explore the clinical relevance of microRNAs in cancer progression by analyzing their expression profiles in patient samples and correlating them with clinicopathological parameters and patient outcomes. This translational aspect of the research will provide valuable insights into the potential use of microRNAs as biomarkers for cancer diagnosis, prognosis, and treatment response. Overall, the project on the analysis of microRNAs in cancer progression seeks to deepen our understanding of the molecular mechanisms underlying cancer development and progression. By shedding light on the roles of specific microRNAs in driving tumorigenesis, this research has the potential to pave the way for the development of personalized and targeted therapies for cancer patients, ultimately leading to improved clinical outcomes and quality of life.