Home / Biochemistry / Estimation of total serum level of protein and iron in mice infected with malaria parasite (plasmodium berghei, berghei) and treated with tetracycline

Estimation of total serum level of protein and iron in mice infected with malaria parasite (plasmodium berghei, berghei) and treated with tetracycline

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Protein and Iron Metabolism
2.2 Malaria Parasite (Plasmodium Berghei, Berghei)
2.3 Tetracycline and Its Mechanism of Action
2.4 Relationship Between Malaria Infection and Protein/Iron Levels
2.5 Previous Studies on Protein and Iron Levels in Mice
2.6 Effects of Malaria Infection on Protein and Iron Metabolism
2.7 Role of Tetracycline in Treating Malaria
2.8 Impact of Tetracycline on Protein and Iron Levels
2.9 Challenges in Estimating Protein and Iron Levels
2.10 Future Research Directions

Chapter THREE

3.1 Research Design
3.2 Sampling Methods
3.3 Data Collection Procedures
3.4 Data Analysis Techniques
3.5 Ethical Considerations
3.6 Validity and Reliability
3.7 Research Limitations
3.8 Timeframe for the Study

Chapter FOUR

4.1 Analysis of Protein Levels in Infected Mice
4.2 Analysis of Iron Levels in Infected Mice
4.3 Comparison of Protein and Iron Levels Pre and Post-Treatment
4.4 Impact of Tetracycline on Protein Metabolism
4.5 Impact of Tetracycline on Iron Metabolism
4.6 Factors Influencing Protein and Iron Levels
4.7 Discussion on Treatment Efficacy
4.8 Implications of Findings on Malaria Treatment

Chapter FIVE

5.1 Summary of Findings
5.2 Conclusion
5.3 Recommendations for Future Research
5.4 Practical Implications
5.5 Contribution to Scientific Knowledge

Thesis Abstract

The serum has been a center of infection of all sorts, in which most diseases associated with the body revolve, causing alterations in the body’s system. Malaria, as a disease condition of the body (especially in mice) results from inflections with the malaria parasite introduced into the blood stream. (malaria parasite introduced is plasmodium berghei, berghei). Parameters related to the blood of which investigation was concerned were those involving serum protein and iron. A total of 20 mice, 15 with malaria infection, and 5 are non-infected mice (mice) also treated with tetracycline were used for the analysis. The following results were obtained in the end of the study and they are as follows; five animals infected, treated with (10mg/kg, underdose) of tetracycline has the total serum proteins and iron of mean and standard deviation for infected as 3.79 ± 0.96 and 2125.72 ± 947.5 respectively but the mean and standard deviation of control group were, (3.83 ± 1.33) and (1184.2 ± 473.6). At (20mg/kg normal dose = 3.95 ± 0.65) and 1547.14 and the control group were 3.83 ± 1.33 and 1184.2 ± 473.6. At (30mg/kg overdose) has the mean and standard deviation of total proteins and iron as 3.78 ± 0.55 and 1168.58 ± 360. 9 respectively. The reduction in iron level as the dose of the drugs increased is as a result of the chelating ability of tetracycline, significant changes also occurred for proteins. The biochemical implications of these results are these discussed.

Thesis Overview

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