Thesis Abstract

Abstract
Cancer remains a pressing global health concern, with a critical need for deeper understanding of its mechanisms to develop more effective diagnostic and therapeutic strategies. MicroRNAs (miRNAs) have emerged as key players in cancer development and progression due to their regulatory role in gene expression. This thesis delves into the exploration of the role of miRNAs in cancer, focusing on their impact on tumor initiation, growth, metastasis, and response to treatment. The introductory chapter lays the foundation by providing background information on miRNAs, cancer biology, and the significance of studying miRNAs in cancer research. The problem statement highlights the gaps in current knowledge and the need to elucidate the specific roles of miRNAs in different types of cancer. The objectives of the study aim to investigate the specific miRNAs involved in cancer development, their mechanisms of action, and their potential as therapeutic targets. The literature review chapter synthesizes existing research on miRNAs in cancer, covering topics such as the dysregulation of miRNAs in various cancer types, their functional roles in oncogenesis, and their diagnostic and prognostic potential. The chapter provides a comprehensive overview of the current understanding of miRNAs in cancer and identifies areas for further investigation. The research methodology chapter outlines the experimental approaches and techniques utilized in this study, including cell culture experiments, miRNA profiling, bioinformatics analysis, and in vivo studies. The methodology aims to investigate the expression levels of specific miRNAs in cancer cells, elucidate their target genes, and assess their functional effects on cancer progression. The discussion of findings chapter presents the results of the study, including the identification of key miRNAs associated with cancer development, their downstream targets, and their effects on cellular processes such as proliferation, apoptosis, and metastasis. The chapter also discusses the implications of these findings for cancer diagnosis, prognosis, and therapy. In conclusion, this thesis provides valuable insights into the role of miRNAs in cancer development and progression, highlighting their potential as biomarkers and therapeutic targets. By elucidating the mechanisms by which miRNAs contribute to cancer pathogenesis, this research contributes to the growing body of knowledge aimed at improving cancer diagnosis and treatment strategies. Keywords microRNAs, cancer, oncogenesis, biomarkers, therapeutic targets, gene expression, tumor progression, metastasis, diagnosis, treatment.

Thesis Overview

The project titled "Exploring the Role of MicroRNAs in Cancer Development and Progression" aims to investigate the intricate involvement of MicroRNAs (miRNAs) in the complex processes of cancer initiation, development, and progression. MiRNAs are small non-coding RNA molecules that play crucial roles in regulating gene expression post-transcriptionally. Over the years, research has highlighted the dysregulation of miRNAs in various cancers, indicating their potential as biomarkers for cancer diagnosis, prognosis, and therapeutic targets. This research project seeks to delve deeper into the specific roles that miRNAs play in promoting or inhibiting cancer development and progression. By exploring the molecular mechanisms underlying the interactions between miRNAs and their target genes, this study aims to elucidate how these regulatory molecules contribute to the hallmarks of cancer, such as uncontrolled cell growth, evasion of cell death, and metastasis. The project will involve a comprehensive literature review to gather existing knowledge on the role of miRNAs in different types of cancer. By critically analyzing and synthesizing the findings from previous studies, this research will identify key miRNAs associated with specific cancer types and their functional significance in tumor biology. Furthermore, this study will employ advanced molecular biology techniques and bioinformatics tools to experimentally validate the functional impact of selected miRNAs on cancer cell behavior. By conducting in vitro and in vivo experiments, the project aims to elucidate the downstream signaling pathways influenced by dysregulated miRNAs and their effects on cancer cell proliferation, migration, and invasion. The findings from this research endeavor are expected to provide valuable insights into the potential diagnostic and therapeutic applications of miRNAs in cancer management. By understanding the specific roles of miRNAs in driving cancer progression, this project aims to contribute to the development of novel miRNA-based strategies for early cancer detection, personalized treatment approaches, and targeted therapy interventions. Overall, the project "Exploring the Role of MicroRNAs in Cancer Development and Progression" seeks to advance our understanding of the complex interplay between miRNAs and cancer pathogenesis, with the ultimate goal of translating this knowledge into clinical applications that improve the diagnosis and treatment outcomes for cancer patients.