Thesis Abstract

Abstract
Cancer is a complex and multifactorial disease that continues to pose significant challenges in terms of diagnosis and treatment. In recent years, there has been growing interest in the role of microRNAs (miRNAs) in cancer development and progression. This thesis aims to explore the intricate relationship between miRNAs and cancer, with a specific focus on how these small non-coding RNAs contribute to the initiation and progression of various types of cancer. Chapter 1 provides an introduction to the study, offering a background on miRNAs and their role in gene regulation. The problem statement highlights the need to better understand the specific mechanisms by which miRNAs influence cancer development. The objectives of the study are outlined to investigate the impact of miRNAs on cancer pathways and to identify potential therapeutic targets. The limitations and scope of the study are discussed, along with the significance of exploring the role of miRNAs in cancer. The structure of the thesis is also presented, providing an overview of the organization of the subsequent chapters. Chapter 2 presents a comprehensive literature review that synthesizes existing research on miRNAs and cancer. Ten key themes emerge from the literature, including the dysregulation of miRNAs in cancer, their role in tumor initiation and progression, and their potential as diagnostic and prognostic biomarkers. Chapter 3 details the research methodology employed in this study, encompassing various aspects such as sample collection, miRNA profiling techniques, data analysis, and statistical methods. Eight key components of the research methodology are discussed in depth to provide a clear understanding of the experimental approach taken. Chapter 4 delves into the discussion of findings, presenting the results of the study and their implications for understanding the role of miRNAs in cancer development and progression. Various miRNAs are identified as key players in specific cancer types, shedding light on potential therapeutic targets and diagnostic markers. Chapter 5 concludes the thesis by summarizing the key findings and implications of the study. The significance of the research in advancing our understanding of the role of miRNAs in cancer is highlighted, along with suggestions for future research directions. Overall, this thesis contributes to the growing body of knowledge on miRNAs and their potential applications in cancer diagnosis and treatment.

Thesis Overview

The project titled "Exploring the Role of MicroRNAs in Cancer Development and Progression" aims to investigate the intricate involvement of MicroRNAs (miRNAs) in the processes of cancer initiation, progression, and metastasis. MiRNAs are small non-coding RNAs that play crucial regulatory roles in gene expression by targeting specific mRNAs for degradation or translational repression. Dysregulation of miRNAs has been implicated in various cancer types, influencing key cellular processes such as proliferation, apoptosis, invasion, and metastasis. This research project will delve into the specific miRNAs that are dysregulated in different stages of cancer development, with a focus on their impact on oncogenic pathways and tumor suppressor mechanisms. By elucidating the functions of these miRNAs, the study aims to provide insights into the underlying molecular mechanisms driving cancer progression and identify potential therapeutic targets for intervention. The methodology will involve a comprehensive literature review to identify key miRNAs associated with different cancer types and their validated target genes. Experimental approaches such as cell culture studies, molecular biology techniques, and bioinformatics analyses will be employed to validate the functional roles of selected miRNAs in cancer cell lines and animal models. The research will also investigate the crosstalk between miRNAs and signaling pathways involved in cancer progression, shedding light on the complex regulatory networks underlying tumorigenesis. The significance of this study lies in its potential to uncover novel biomarkers for cancer diagnosis and prognosis, as well as therapeutic targets for precision medicine approaches. By elucidating the role of miRNAs in cancer development and progression, this research aims to contribute to the growing body of knowledge on the molecular mechanisms driving oncogenesis and metastasis. Overall, the project "Exploring the Role of MicroRNAs in Cancer Development and Progression" seeks to advance our understanding of the intricate interplay between miRNAs and cancer biology, with the ultimate goal of identifying new avenues for targeted cancer therapies and improving patient outcomes in the fight against cancer.