Thesis Abstract

Abstract
MicroRNAs (miRNAs) are small non-coding RNA molecules that play crucial roles in regulating gene expression and various cellular processes. In recent years, the dysregulation of miRNAs has been associated with cancer development and progression, making them potential biomarkers and therapeutic targets for cancer treatment. This thesis aims to explore the role of miRNAs in cancer development and progression, focusing on their mechanisms of action, diagnostic potentials, and therapeutic applications. The introduction provides a comprehensive overview of miRNAs, cancer biology, and the significance of studying miRNAs in cancer research. The background of the study delves into the molecular mechanisms underlying miRNA dysregulation in cancer, highlighting the complex interplay between miRNAs and cancer-related signaling pathways. The problem statement emphasizes the need to elucidate the specific roles of miRNAs in different types of cancer to enhance our understanding of cancer pathogenesis and improve patient outcomes. The objectives of the study include identifying key miRNAs involved in cancer development, evaluating their diagnostic and prognostic value, and exploring the therapeutic potential of targeting miRNAs in cancer treatment. The limitations of the study are acknowledged, such as the challenges associated with miRNA target prediction and validation, as well as the heterogeneity of cancer types and patient populations. The scope of the study outlines the specific cancer types and miRNAs that will be investigated to achieve the research objectives. The significance of the study lies in its potential to advance our knowledge of the molecular mechanisms driving cancer progression and identify novel strategies for cancer diagnosis and therapy. The structure of the thesis provides a roadmap for the reader, outlining the organization of the chapters and the flow of the research work. Definitions of key terms related to miRNAs and cancer biology are provided to facilitate understanding throughout the thesis. The literature review chapter synthesizes existing research on miRNAs in cancer, covering topics such as miRNA biogenesis, mechanisms of miRNA dysregulation in cancer, and the clinical implications of miRNA biomarkers. The research methodology chapter describes the experimental approaches and bioinformatics tools used to investigate miRNA expression profiles, target genes, and functional roles in cancer cells. The discussion of findings chapter presents the results of miRNA profiling studies, functional analyses, and clinical correlations, highlighting the key findings and their implications for cancer research and therapy. In conclusion, this thesis contributes to the growing body of knowledge on the role of miRNAs in cancer development and progression, shedding light on the complex regulatory networks that govern cancer cell behavior. By elucidating the mechanisms by which miRNAs influence cancer pathogenesis, this research has the potential to identify new therapeutic targets and diagnostic markers for precision cancer medicine. The summary encapsulates the main findings and implications of the study, emphasizing the importance of further research in this field to translate miRNA discoveries into clinical applications for cancer patients.

Thesis Overview

The project titled "Exploring the Role of MicroRNAs in Cancer Development and Progression" aims to investigate the intricate relationship between microRNAs and the development and progression of cancer. MicroRNAs are small non-coding RNA molecules that play crucial roles in regulating gene expression, and their dysregulation has been associated with various types of cancers. This research seeks to deepen our understanding of how specific microRNAs contribute to the initiation, growth, and spread of cancer cells, with the ultimate goal of identifying potential targets for therapeutic interventions. The research will begin with a comprehensive literature review to examine the current state of knowledge regarding the roles of microRNAs in cancer. This review will cover key studies that have identified specific microRNAs as biomarkers for different cancer types and have elucidated their mechanisms of action in promoting or inhibiting tumor growth. By synthesizing existing research findings, the study aims to identify gaps in knowledge and areas where further investigation is needed. The methodology section of the research will outline the experimental approaches that will be employed to investigate the functions of specific microRNAs in cancer development and progression. This will include techniques such as cell culture experiments, molecular biology assays, and bioinformatics analyses to study the expression patterns and targets of selected microRNAs in cancer cells. By utilizing a multidisciplinary approach, the research aims to provide a comprehensive understanding of the molecular mechanisms underlying the roles of microRNAs in cancer. The findings of the study will be presented and discussed in detail in the results and discussion section. This section will highlight the specific microRNAs that are found to be dysregulated in different types of cancer and their impact on key cellular processes such as proliferation, invasion, and metastasis. The research will also explore the potential therapeutic implications of targeting these dysregulated microRNAs for cancer treatment, including the development of novel therapeutic strategies based on microRNA modulation. In conclusion, this research project on the role of microRNAs in cancer development and progression seeks to contribute to the growing body of knowledge on the molecular mechanisms underlying cancer pathogenesis. By elucidating the functions of specific microRNAs in promoting or suppressing cancer growth, the study aims to identify novel targets for therapeutic intervention and ultimately improve outcomes for cancer patients. By shedding light on the complex interplay between microRNAs and cancer, this research has the potential to pave the way for the development of innovative precision medicine approaches for cancer treatment."