Thesis Abstract

Abstract
Telomeres are essential structures at the ends of chromosomes that play a crucial role in maintaining genomic stability and cellular function. The length of telomeres has been associated with cellular aging and various age-related diseases. This study aimed to investigate the relationship between telomere length and cellular aging in human cells. The research methodology involved the collection of human cell samples from different age groups, ranging from young adults to elderly individuals. Telomere length was measured using quantitative polymerase chain reaction (qPCR) and compared across the age groups. Additionally, the expression levels of key genes involved in telomere maintenance and cellular aging were analyzed using gene expression profiling techniques. The findings of this study revealed a significant correlation between telomere length and age, with older individuals exhibiting shorter telomeres compared to younger individuals. Furthermore, gene expression analysis identified dysregulation of telomere maintenance genes in aged cells, suggesting a potential mechanism underlying telomere shortening during cellular aging. The discussion of the findings delved into the implications of telomere length dynamics in cellular aging and its potential relevance to age-related diseases such as cancer and cardiovascular disorders. The limitations of the study, including sample size constraints and technical challenges in telomere length measurement, were also addressed. In conclusion, this study provides valuable insights into the relationship between telomere length and cellular aging in human cells. The results highlight the importance of maintaining telomere integrity for healthy aging and underscore the potential therapeutic targets for age-related diseases. Further research in this area is warranted to elucidate the underlying mechanisms and explore interventions to preserve telomere length and promote healthy aging.

Thesis Overview

The project titled "Analysis of Telomere Length and Its Relationship to Cellular Aging in Human Cells" focuses on investigating the correlation between telomere length and cellular aging in human cells. Telomeres are repetitive DNA sequences located at the ends of chromosomes that play a crucial role in maintaining genomic stability and regulating cellular lifespan. As cells divide, telomeres progressively shorten, eventually leading to cellular senescence or programmed cell death. The research seeks to explore how variations in telomere length impact the aging process of human cells. By analyzing telomere dynamics in different cell types and under varying conditions, the study aims to elucidate the mechanisms underlying cellular aging and its implications for overall health and disease development. Understanding the relationship between telomere length and cellular aging is essential for advancing knowledge in the field of biochemistry and molecular biology. Through a combination of experimental techniques, including cell culture, molecular biology assays, and bioinformatics analyses, the project will investigate how telomere length influences key cellular processes such as proliferation, differentiation, and cell death. By examining the interplay between telomere maintenance mechanisms and cellular aging pathways, the research aims to provide insights into potential strategies for promoting healthy aging and preventing age-related diseases. Overall, the project on the analysis of telomere length and its relationship to cellular aging in human cells represents a critical endeavor to deepen our understanding of the molecular mechanisms underlying aging and longevity. By shedding light on the role of telomeres in cellular aging, the research aims to contribute valuable knowledge to the fields of biochemistry, cell biology, and aging research, with potential implications for therapeutic interventions targeting age-related disorders.