Thesis Abstract

Abstract
MicroRNAs (miRNAs) have emerged as pivotal regulators of gene expression in various biological processes, including cancer development and progression. This thesis investigates the role of miRNAs in regulating gene expression specifically in cancer cells. The study aims to explore the complex interplay between miRNAs and their target genes in cancer, shedding light on potential mechanisms underlying cancer pathogenesis. Chapter One provides a comprehensive introduction to the research topic, outlining the background of the study, identifying the problem statement, setting the objectives, discussing the limitations and scope of the study, highlighting the significance of the research, and defining key terms. Chapter Two presents a detailed literature review encompassing ten key aspects related to miRNAs, gene expression regulation, and cancer biology. This section synthesizes existing knowledge and gaps in the field, providing a solid foundation for the research study. Chapter Three delves into the research methodology, detailing the experimental design, sample collection, miRNA profiling techniques, bioinformatics analysis, validation methods, and statistical approaches used to investigate miRNA-mediated gene regulation in cancer cells. Chapter Four presents an in-depth discussion of the research findings, analyzing the impact of specific miRNAs on gene expression patterns in different types of cancer cells. The chapter explores the functional roles of miRNAs in cancer progression, metastasis, and drug resistance, offering insights into potential therapeutic targets. Finally, Chapter Five offers a conclusive summary of the thesis, highlighting key findings, discussing the implications of the research, and proposing future directions for further exploration in the field of miRNA-mediated gene regulation in cancer cells. The study contributes to a better understanding of the molecular mechanisms underlying cancer development and provides valuable insights for the development of novel diagnostic and therapeutic strategies in oncology. Keywords microRNAs, gene expression, cancer cells, regulatory mechanisms, oncology, molecular biology.

Thesis Overview

The project titled "Investigation of the role of microRNAs in regulating gene expression in cancer cells" aims to explore the intricate relationship between microRNAs and gene expression in the context of cancer. MicroRNAs are small non-coding RNA molecules that play a crucial role in post-transcriptional gene regulation by targeting messenger RNA for degradation or translational repression. In cancer, dysregulation of microRNAs can lead to aberrant gene expression patterns, contributing to tumorigenesis, progression, and metastasis. This research project will delve into the specific mechanisms through which microRNAs influence gene expression in cancer cells. By analyzing the expression profiles of microRNAs and target genes in various cancer types, the study seeks to identify key regulatory networks and pathways involved in cancer development. Understanding how microRNAs modulate gene expression in cancer cells can provide valuable insights into potential therapeutic targets and biomarkers for cancer diagnosis and treatment. The project will employ a combination of molecular biology techniques, bioinformatics analysis, and cell culture experiments to investigate the functional roles of specific microRNAs in regulating gene expression in cancer cells. By elucidating the molecular mechanisms underlying the interactions between microRNAs and target genes, the study aims to uncover novel biomarkers and therapeutic strategies for combating cancer progression. Overall, this research project represents a significant contribution to the field of cancer biology by shedding light on the pivotal role of microRNAs in shaping gene expression patterns in cancer cells. The findings from this study have the potential to advance our understanding of cancer biology and pave the way for the development of more targeted and effective therapies for cancer patients.