Thesis Abstract

Abstract
MicroRNAs (miRNAs) are small non-coding RNA molecules that play crucial roles in post-transcriptional gene regulation by targeting messenger RNAs (mRNAs) for degradation or translational repression. In cancer, dysregulation of miRNAs has been implicated in various aspects of tumorigenesis, including cell proliferation, apoptosis, and metastasis. This thesis aims to explore the role of miRNAs in regulating gene expression in cancer cells, with a focus on understanding how aberrant miRNA expression contributes to cancer development and progression. Chapter One provides an introduction to the study, presenting the background of miRNAs, the problem statement, objectives of the study, limitations, scope, significance, structure of the thesis, and definitions of key terms. Chapter Two comprises a comprehensive literature review covering ten key aspects related to miRNAs in cancer, including their biogenesis, function, and involvement in different cancer types. Chapter Three details the research methodology employed in this study, including the selection of cell lines, miRNA profiling techniques, and bioinformatics analysis methods. This chapter also discusses the experimental design and data analysis approaches used to investigate the regulatory role of miRNAs in cancer cells. Chapter Four presents a detailed discussion of the findings obtained from the research, highlighting specific miRNAs that are dysregulated in cancer cells and their target genes involved in cancer-related pathways. The chapter delves into the molecular mechanisms underlying the interaction between miRNAs and their target mRNAs, providing insights into how these interactions influence cancer cell behavior. In Chapter Five, the conclusion and summary of the thesis are provided, summarizing the key findings, implications of the research, and potential future directions in the field. The study contributes to our understanding of the intricate regulatory networks mediated by miRNAs in cancer cells and their implications for cancer biology and therapy. Overall, this thesis sheds light on the critical role of miRNAs in cancer pathogenesis and provides a foundation for further research aimed at exploiting miRNAs as potential diagnostic markers and therapeutic targets in cancer treatment.

Thesis Overview

The research project titled "The Role of MicroRNAs in Regulating Gene Expression in Cancer Cells" aims to investigate the intricate mechanisms by which microRNAs influence gene expression in cancer cells. MicroRNAs are small non-coding RNAs that play crucial roles in post-transcriptional gene regulation by targeting messenger RNA (mRNA) for degradation or translational repression. In the context of cancer, dysregulation of microRNAs has been implicated in tumor initiation, progression, and metastasis. The project will begin with a comprehensive introduction providing background information on microRNAs, their biogenesis, and their functions in normal cellular processes. The problem statement will highlight the significance of understanding how dysregulated microRNAs contribute to the development and progression of cancer. The objectives of the study will outline specific research goals, such as identifying key microRNAs involved in cancer pathogenesis and elucidating their downstream targets. Limitations of the study will be acknowledged, such as the complexity of gene regulatory networks and the challenges associated with experimental validation of microRNA-mRNA interactions. The scope of the study will define the boundaries within which the research will be conducted, focusing on specific cancer types or molecular pathways. The significance of the study will underscore the potential impact of the findings on advancing our knowledge of cancer biology and identifying novel therapeutic targets. The structure of the thesis will be outlined to provide a roadmap for the reader, detailing the organization of chapters and key sections. Definitions of terms will clarify any technical terminology used throughout the research work to ensure clarity and understanding. The literature review will delve into existing studies on microRNAs in cancer, highlighting key findings, gaps in knowledge, and emerging trends in the field. This section will provide a comprehensive overview of the current understanding of microRNA-mediated gene regulation in cancer cells. The research methodology will detail the experimental approaches and techniques employed to investigate the role of microRNAs in cancer. This section will include information on cell culture models, molecular biology assays, bioinformatics analyses, and any other methods used to study microRNA function in cancer cells. The discussion of findings will present and interpret the results obtained from the research, linking them back to the research objectives and existing literature. This section will analyze the implications of the findings and their potential relevance to cancer biology and therapy. In the conclusion and summary, the key findings of the study will be summarized, along with their implications for understanding the role of microRNAs in regulating gene expression in cancer cells. Future research directions and potential applications of the findings will also be discussed to guide further investigations in this field. Overall, this research project seeks to enhance our understanding of the complex interplay between microRNAs and gene expression in cancer cells, with the ultimate goal of identifying novel therapeutic strategies to target dysregulated microRNAs in cancer treatment.